Adipose-Derived Stem Cells (ASCs) for Tissue Engineering

Tremp, Mathias; Salemi, Souzan; Gobet, Rita; Sulser, Tullio; Eberli, Daniel

doi:10.5772/22120

Cited by 12 publications

(9 citation statements)

References 96 publications

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“…To achieve this, effective cell isolation and expansion of harvested cells are of utmost importance. Inadequate isolation methods can lead to polluted isolates and hence inconsistency in cell marker expression [23]. Further, long‐term expansion has demonstrated decreased stem cell proliferation and differentiation capacity [24].…”

Section: Critical Proceduresmentioning

confidence: 99%

Concise Review: Cell-Based Strategies in Bone Tissue Engineering and Regenerative Medicine

Both

Yang

et al. 2013

Stem Cells Translational Medicine

149

100

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Cellular strategies play an important role in bone tissue engineering and regenerative medicine (BTE/RM). Variability in cell culture procedures (e.g., cell types, cell isolation and expansion, cell seeding methods, and preculture conditions before in vivo implantation) may influence experimental outcome. Meanwhile, outcomes from initial clinical trials are far behind those of animal studies, which is suggested to be related to insufficient nutrient and oxygen supply inside the BTE/RM constructs as some complex clinical implementations require bone regeneration in too large a quantity. Coculture strategies, in which angiogenic cells are introduced into osteogenic cell cultures, might provide a solution for improving vascularization and hence increasing bone formation for cell-based constructs. So far, preclinical studies have demonstrated that cell-based tissue-engineered constructs generally induce more bone formation compared with acellular constructs. Further, cocultures have been shown to enhance vascularization and bone formation compared with monocultures. However, translational efficacy from animal studies to clinical use requires improvement, and the role implanted cells play in clinical bone regeneration needs to be further elucidated. In view of this, the present review provides an overview of the critical procedures during in vitro and in vivo phases for cell-based strategies (both monoculture and coculture) in BTE/RM to achieve more standardized culture conditions for future studies, and hence enhance bone formation.

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Section: Critical Proceduresmentioning

confidence: 99%

Concise Review: Cell-Based Strategies in Bone Tissue Engineering and Regenerative Medicine

Both

Yang

et al. 2013

Stem Cells Translational Medicine

149

100

View full text Add to dashboard Cite

show abstract

“…The pluripotency and unlimited self-renewal capacities exhibited by stem cells pose great promise in tissue engineering and are expected to significantly contribute to tissue reconstruction applications (Bianco & Robey, 2001). The potential of using autologous fat tissue-derived mesenchymal stem cells (MSC) is rapidly becoming a clinical reality (Tremp, Eberli, Gobet, Salemi, & Sulser, 2011). The use of such an abundant extraneous tissue as an autologous cell source has significant implications for plastic and reconstructive surgeons (Cherubino & Marra, 2009;Sterodimas, de Faria, Nicaretta, & Pitanguy, 2010), as it will enable the use of liposuction waste as a starting material (Cao et al, 2005;Huang & Hsu, 2013).…”

Section: Introductionmentioning

confidence: 99%

Engineering vascularized flaps using adipose-derived microvascular endothelial cells and mesenchymal stem cells

Freiman

Shandalov

Rosenfeld

et al. 2017

J Tissue Eng Regen Med

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Human adipose-derived microvascular endothelial cells (HAMEC) and mesenchymal stem cells (MSC) have been shown to bear angiogenic and vasculogenic capabilities. We hypothesize that co-culturing HAMEC:MSC on a porous biodegradable scaffold in vitro, later implanted as a graft around femoral blood vessels in a rat, will result in its vascularization by host vessels, creating a functional vascular flap that can effectively treat a range of large full-thickness soft tissue defects.HAMEC were co-cultured with MSC on polymeric three-dimensional porous constructs. Grafts were then implanted around the femoral vessels of a rat. To ensure vessel sprouting from the main femoral vessels, grafts were pre-isolated from the surrounding tissue. Graft vascularization was monitored to confirm full vascularization before flap transfer. Flaps were then transferred to treat both abdominal wall and exposed bone and tendon of an ankle defects. Flaps were analysed to determine vascular properties in terms of maturity, functionality and survival of implanted cells. Findings show that pre-isolated grafts bearing the HAMEC:MSC combination promoted formation of highly vascularized flaps, which were better integrated in both defect models. The results of this study show the essentiality of a specific adipose-derived cell combination in successful graft vascularization and integration, two processes crucial for flap survival. Copyright

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“…Adipose‐derived stem cells (ASCs) can differentiate into cells of adipogenic , chondrogenic , myogenic , osteogenic , hepatic and neurogenic lineages. The relatively high frequency of clonogenic cells and their acquisition from adipose tissue , have made them an appealing cell source for tissue engineering . Many studies have indicated that ASCs can be ostegenically induced by mechanical stress and results from our previous studies also have shown that cyclic tensile mechanical loading can promote their osteogenic differentiation in osteogenic medium .…”

Section: Introductionmentioning

confidence: 85%

Osteogenic differentiation of adipose‐derived stem cells prompted by low‐intensity pulsed ultrasound

et al. 2013

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Adipose-Derived Stem Cells (ASCs) for Tissue Engineering

Cited by 12 publications

References 96 publications

Concise Review: Cell-Based Strategies in Bone Tissue Engineering and Regenerative Medicine

Concise Review: Cell-Based Strategies in Bone Tissue Engineering and Regenerative Medicine

Engineering vascularized flaps using adipose-derived microvascular endothelial cells and mesenchymal stem cells

Osteogenic differentiation of adipose‐derived stem cells prompted by low‐intensity pulsed ultrasound

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