Adipose Derived Stem Cells: Current State of the Art and Prospective Role in Regenerative Medicine and Tissue Engineering

Cardiomyogenic differentiation of human sternal bone marrow mesenchymal stem cells using a combination of basic fibroblast growth factor and hydrocortisone

“…Results are presented as mean AE SD from three biological replicates. Hydrocortisone has been found to play a critical role in the regulation of cardiomyocyte proliferation (Vindigni et al, 2013) and cell differentiation toward the myogenic lineages (Zuk et al, 2002). Consistent with their findings, MSCs were successfully differentiated into cardiomyocytes as characterized by the expression of cardiac markers in both protein and mRNA levels.…”

“…However, Khezri et al (2007) evaluated the possible biological role of bFGF in the differentiation of mouse ESCs into cardiomyocytes and narrated that bFGF alone is incapable of inducing the development of cardiomyocytes, indicating that combination with other factors is necessary for cardiomyocyte differentiation. Hydrocortisone has been found to play a critical role in the regulation of cardiomyocyte proliferation (Vindigni et al, 2013) and cell differentiation toward the myogenic lineages (Zuk et al, 2002). In addition, our own unpublished results showed that in combination, bFGF and hydrocortisone successfully induce differentiation of sheep marrow MSCs into cardiomyocytes.…”

“…Bone morphogenic protein (BMP-2/4), activin A (Han et al, 2011), insulin like growth factor (IGF-1), and fibroblast growth factor (FGF-2;Franco et al, 2011;Asumda and Chase, 2012;Asumda, 2013) are few examples that have been tested to induce cardiomyogenic differentiation of multiple stem cell sources, including embryonic stem cells, induced-pluripotent stem cells, and MSCs. Hydrocortisone, a stress hormone produced by the adrenal gland, has been shown to stimulate the entry of cardiomyocytes into division cycle, and thus enhances their proliferation in vitro (Zuk et al, 2002;Giraud et al, 2006;Vindigni et al, 2013). Hydrocortisone, a stress hormone produced by the adrenal gland, has been shown to stimulate the entry of cardiomyocytes into division cycle, and thus enhances their proliferation in vitro (Zuk et al, 2002;Giraud et al, 2006;Vindigni et al, 2013).…”

“…Basic fibroblast growth factor (bFGF) is a member of heparin binding growth factor and is known to have an important role in driving MSCs to the cardiomyogenic lineage during embryogenesis Rosenblatt-Velin et al, 2005). Hydrocortisone, a stress hormone produced by the adrenal gland, has been shown to stimulate the entry of cardiomyocytes into division cycle, and thus enhances their proliferation in vitro (Zuk et al, 2002;Giraud et al, 2006;Vindigni et al, 2013).…”

Hyperbaric Oxygen Therapy Improves the Osteogenic and Vasculogenic Properties of Mesenchymal Stem Cells in the Presence of Inflammation In Vitro

“…In detail, the aim of the present work was to analyze the direct effects of HBO on the metabolic activity and on the osteogenic and vasculogenic differentiation properties of human Adipose-Derived Stem Cells (hADSCs) grown in a simulated pro-inflammatory environment. MSCs isolated from human adipose tissue samples share many properties with those harvested from bone marrow, especially the same differentiation potential and a similar surface markers expression [20]. In addition, hADSCs represent a source of several cytokines and soluble factors with angiogenic, antiapoptotic, and antioxidative properties, and also act as modulators of the immune system, by inhibiting the secretion of inflammatory cytokines while stimulating the production of those with anti-inflammatory activity [21].…”