Adipose‐derived stem cells for wound healing

Hassanshahi, Alireza; Hassanshahi, Mohammadhossein; Khabbazi, Samira; Hosseini‐Khah, Zahra; Peymanfar, Yaser; Ghalamkari, Saman; Su, Yu Wen; Chen, Xian

doi:10.1002/jcp.27922

Cited by 157 publications

(124 citation statements)

References 161 publications

(250 reference statements)

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“…A series of studies have uncovered the probable pathophysiology of diabetic wounds, which includes decreased growth factor production, impaired angiogenesis and defective cellular functions in the impaired wound [26]. The current conventional treatments, such as application of dressings, negative pressure therapy, autologous skin graft and hyperbaric oxygen therapy, have several limitations and moderate efficacy [7].…”

Section: Discussionmentioning

confidence: 99%

“…It has been proven that stem cell therapy can contribute to diabetic wounds and other diseases [4][5][6]. Compared with other types of stem cells, adipose-derived stem cells (ADSCs) have specific advantages and fewer limitations; they are abundant and easily harvested, can be delivered via a minimally invasive procedure, are associated with fewer ethical issues and have less risk of inducing tumorigenicity and the host immune response [7,8]. A large number of studies have verified the therapeutic efficacy of ADSCs for wound healing [9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

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Adipose-derived stem cells combined with platelet-rich plasma enhance wound healing in a rat model of full-thickness skin defects

Shan

et al. 2020

Preprint

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Background: Wound healing is impaired in patients with diabetes due to the multifactorial etiology of the disease, which limits the therapeutic efficacy of various approaches. This study hypothesizes that the combination of adipose-derived stem cells (ADSCs) and platelet-rich plasma (PRP) might achieve optimally efficient diabetic wound healing. Methods: ADSCs were isolated from the adipose tissues of Sprague-Dawley (SD) rats. PRP was prepared by using a two-step centrifugation technique. A diabetic wound model was established on the backs of SD rats to evaluate the effect of ADSCs incorporated into PRP. Hematoxylin and eosin staining, immunofluorescence and immunohistochemistry were performed to observe the changes in neovascularization. The proliferation of endothelial cells was assessed by the MTS assay. Results: ADSCs incorporated into PRP induced a higher wound closure rate than ADSCs, PRP and negative control. The expression levels of VEGF and SDF-1 in the ADSC-PRP group were higher than those in the other groups. Moreover, the proliferation of endothelial cells was strongly stimulated by treatment with the combination of ADSC-conditioned medium (ADSC-CM) and PRP. Conclusions: PRP enhanced diabetic wound healing induced by ADSCs, and its promoting effect involved neovascularization. Keywords: Adipose-derived stem cell, Platelet-rich plasma, Diabetic wound healing, Neovascularization.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Adipose-derived stem cells combined with platelet-rich plasma enhance wound healing in a rat model of full-thickness skin defects

Shan

et al. 2020

Preprint

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“…Many studies focused on skin wound healing. Stem cells were used to cure wound healing [5]. In our study, BMSCs and HSCs were used to cure wound healing in mice.…”

Section: Discussionmentioning

confidence: 99%

“…Mesenchymal stem cells and hematopoietic stem cells are often injected in blood or around the wound. However, these methods have di culties in tissue targeting and high cell attrition rate [5]. Very few studies had shown bone mesenchymal stem cells(BMSC) and hematopoietic stem cells(HSC) could arrive to the wound and differentiate into tissues [6].…”

Section: Introductionmentioning

confidence: 99%

Hematopoietic stem cells can differentiate into pericytes and myofibroblast in wound healing, not bone Mesenchymal stem cells

Kong

Cheng

Xuan³

et al. 2020

Preprint

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Background Hematopoietic stem cells(HSCs) and mesenchymal stem cells(MSCs) can participate in wound healing. However, very few studies had shown HSCs and MSCs could arrive to the wound and differentiate into tissues. In this study, we intend to investigate the role of bone marrow HSCs and MSCs in wound healing. Methods We first removed the bone marrow of mice by irradiation. Furthermore, we injected different colours of fluorescent HSCs and MSCs into the tail vein of irradiated mice to reconstruct bone marrow function. We prepared wound models on the back of these mice. In vivo imaging and immunohistochemical staining were used to track the expression of fluorescent protein. Results HSCs and MSCs have been isolated and cultured. HSCs expressed expressed Sca1, not lineage, CD34 or CD48. MSCs expressed expressed CD29 and CD44,not CD34 or CD45. HSCs labeled with green fluorescent protein reached the wound and co-expressed with desmin and α-SMA. MSCs didn’t stay on the wound. Conclusions The results show HSCs in the bone marrow of mice can directly participate in wound healing and differentiate into pericytes and myofibroblasts.

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“…Compared with bone marrow-derived stem cells (BMSCs), hADSCs are favored in stem cell therapy for their advantages in terms of expansion ability, easy of isolation, and survival rate during culturing. Therefore, hADSCs have been considered as an attractive cell type for MSC transplantation therapy in regenerative medicine [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Diabetes-induced glucolipotoxicity impairs wound healing ability of adipose-derived stem cells-through the miR-1248/CITED2/HIF-1α pathway

Xiao

Zhang

Liu

et al. 2020

Aging

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Despite being an attractive cell type for mesenchymal stem cell (MSC) transplantation therapy for wound healing, human adipose-derived stem cells (hADSCs) from diabetes mellitus (DM) patients result in remarkable retention of stem cell activity due to diabetes-induced glucolipotoxicity. We explored the effect of diabetes and medium containing AGEs on the cell activity, phenotype, multipotency, angiogenic potential, and the therapeutic effect of hADSCs. Then, miRNA-1248 was selected by miRNA microarray analysis to further study the core molecular pathways that regulate the wound healing ability of hADSCs. hADSCs isolated from DM patients or cultured in medium containing AGEs in vitro exhibited decreased effectiveness in stem cell therapy. The expression of miRNA-1248 was decreased in the hADSCs of DM patients and hence failed to positively regulate stem cell activity, differentiation functions, and angiogenesis promotion effect. This concomitantly increased the expression of CITED2, an inhibitor of HIF-1α, thus influencing growth factors that promote angiogenesis, cellular proliferation, and wound healing. Overall, our data demonstrated that the glucolipotoxicity-impaired wound healing ability of hADSCs might occur through the miR-1248/CITED2/HIF-1α pathway. MiRNA-1248 may have potential to be used as a novel therapeutic target for wound healing in DM patients or restoring the wound healing ability of diabetic hADSCs.

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Adipose‐derived stem cells for wound healing

Cited by 157 publications

References 161 publications

Adipose-derived stem cells combined with platelet-rich plasma enhance wound healing in a rat model of full-thickness skin defects

Adipose-derived stem cells combined with platelet-rich plasma enhance wound healing in a rat model of full-thickness skin defects

Hematopoietic stem cells can differentiate into pericytes and myofibroblast in wound healing, not bone Mesenchymal stem cells

Diabetes-induced glucolipotoxicity impairs wound healing ability of adipose-derived stem cells-through the miR-1248/CITED2/HIF-1α pathway

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