Adipose-Derived Stem Cells in Novel Approaches to Breast Reconstruction: Their Suitability for Tissue Engineering and Oncological Safety

O’Halloran, Niamh; Courtney, D.; Kerin, Michael J.; Lowery, Aoife

doi:10.1177/1178223417726777

Cited by 47 publications

(80 citation statements)

References 209 publications

(234 reference statements)

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“…Research has thus focused on the use of primary adult ADSCs, especially autologous ones. These are multipotent cells that have been used since 2001 as a major source of cells with a regenerative potential, becoming the gold standard for regenerative techniques and breast tissue engineering (O'Halloran et al, ; Sterodimas, de Faria, Nicaretta, & Pitanguy, ).…”

Section: In Vitro Researchmentioning

confidence: 99%

“…There are numerous advantages of using autologous adipocytes: biocompatibility, versatility, nonimmunogenicity, and minimal donor site morbidity (Campbell, Hume, & Watson, ; O'Halloran et al, ).…”

Section: In Vitro Researchmentioning

confidence: 99%

“…ADSCs are collected by the centrifugation of the SVF, the product of liposuction, previously digested by collagenase. The SVF contains various cell types such as endothelial precursor cells, endothelial cells, pluripotent vascular progenitors, macrophages, and preadipocytes (O'Halloran et al, ).…”

Section: In Vitro Researchmentioning

confidence: 99%

“…The conservative mastectomy spares the healthy skin envelope with or without the nipple‐area complex. A research group based in Ireland has shown that the remaining skin works as a pocket to facilitate the reconstruction through prosthesis (O'Halloran, Courtney, Kerin, & Lowery, ). Benson et al suggested applying conservative mastectomy to women with a ductal carcinoma in situ, located at least 2 cm aside the nipple‐areola complex (NAC).…”

Section: Introductionmentioning

confidence: 99%

“…As an alternative to these conventional therapies, O'Halloran and collaborators showed the increasing interest in using adipose‐derived stem cells (ADSCs) for breast reconstruction and augmentation (O'Halloran et al, ). These cells show a high regenerative potential and commitment mainly in mature adipocytes, which are able to maintain the viability of the transplanted adipose tissue and provide a more natural volume of the mammary gland.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Tissue engineering and regenerative medicine strategies for the female breast

Conci

Bennati

Bregoli

et al. 2019

J Tissue Eng Regen Med

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The complexity of mammary tissue and the variety of cells involved make tissue regeneration an ambitious goal. This review, supported by both detailed macro and micro anatomy, illustrates the potential of regenerative medicine in terms of mammary gland reconstruction to restore breast physiology and morphology, damaged by mastectomy. Despite the widespread use of conventional therapies, many critical issues have been solved using the potential of stem cells resident in adipose tissue, leading to commercial products. in vitro research has reported that adipose stem cells are the principal cellular source for reconstructing adipose tissue, ductal epithelium, and nipple structures. In addition to simple cell injection, construct made by cells seeded on a suitable biodegradable scaffold is a viable alternative from a long‐term perspective. Preclinical studies on mice and clinical studies, most of which have reached Phase II, are essential in the commercialization of cellular therapy products. Recent studies have revealed that the enrichment of fat grafting with stromal vascular fraction cells is a viable alternative to breast reconstruction. Although in the future, organ‐on‐a‐chip can be envisioned, for the moment researchers are still focusing on therapies that are a long way from regenerating the whole organ, but which nevertheless prevent complications, such as relapse and loss in terms of morphology.

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Section: In Vitro Researchmentioning

confidence: 99%

Section: In Vitro Researchmentioning

confidence: 99%

Section: In Vitro Researchmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Tissue engineering and regenerative medicine strategies for the female breast

Conci

Bennati

Bregoli

et al. 2019

J Tissue Eng Regen Med

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Obesity‐Associated Adipose Stromal Cells Promote Breast Cancer Invasion through Direct Cell Contact and ECM Remodeling

Mulligan

Ouyang

et al. 2020

Adv Funct Materials

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Obesity increases the risk and worsens the prognosis for breast cancer due, in part, to altered adipose stromal cell (ASC) behavior. Whether ASCs from obese individuals increase migration of breast cancer cells relative to their lean counterparts, however, remains unclear. To test this connection, multicellular spheroids composed of MCF10A-derived tumor cell lines of varying malignant potential and lean or obese ASCs are embedded into collagen scaffolds mimicking the elastic moduli of interstitial breast adipose tissue. Confocal image analysis suggests that tumor cells alone migrate insignificantly under these conditions. However, direct cell-cell contact with either lean or obese ASCs enables them to migrate collectively, whereby obese ASCs activate tumor cell migration more effectively than their lean counterparts. Time-resolved optical coherence tomography imaging suggests that obese ASCs facilitate tumor cell migration by mediating contraction of local collagen fibers. Matrix metalloproteinase (MMP)-dependent proteolytic activity significantly contributes to ASC-mediated tumor cell invasion and collagen deformation. However, ASC contractility is also important, as co-inhibition of both MMPs and contractility is necessary to completely abrogate ASC-mediated tumor cell migration. These findings imply that obesity-mediated changes of ASC phenotype may impact tumor cell migration and invasion with potential implications for breast cancer malignancy in obese patients.

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Assessment of human adipose‐derived stem cell on surface‐modified silicone implant to reduce capsular contracture formation

Sutthiwanjampa

Shin

Ryu

et al. 2021

Bioengineering & Transla Med

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Medical devices made from poly(dimethylsiloxane) (PDMS)‐based silicone implants have been broadly used owing to their inert properties, biocompatibility, and low toxicity. However, long‐term implantation is usually associated with complications, such as capsular contracture due to excessive local inflammatory response, subsequently requiring implant removal. Therefore, modification of the silicone surface to reduce a risk of capsular contracture has attracted increasing attention. Human adipose‐derived stem cells (hASCs) are known to provide potentially therapeutic applications for tissue engineering, regenerative medicine, and reconstructive surgery. Herein, hASCs coating on a PDMS (hASC‐PDMS) or itaconic acid (IA)‐conjugated PDMS (hASC‐IA‐PDMS) surface is examined to determine its biocompatibility for reducing capsular contracture on the PDMS surface. In vitro cell cytotoxicity evaluation showed that hASCs on IA‐PDMS exhibit higher cell viability than hASCs on PDMS. A lower release of proinflammatory cytokines is observed in hASC‐PDMS and hASC‐IA‐PDMS compared to the cells on plate. Multiple factors, including in vivo mRNA expression levels of cytokines related to fibrosis; number of inflammatory cells; number of macrophages and myofibroblasts; capsule thickness; and collagen density following implantation in rats for 60 days, indicate that incorporated coating hASCs on PDMSs most effectively reduces capsular contracture. This study demonstrates the potential of hASCs coating for the modification of PDMS surfaces in enhancing surface biocompatibility for reducing capsular contracture of PDMS‐based medical devices.

show abstract

Adipose-Derived Stem Cells in Novel Approaches to Breast Reconstruction: Their Suitability for Tissue Engineering and Oncological Safety

Cited by 47 publications

References 209 publications

Tissue engineering and regenerative medicine strategies for the female breast

Tissue engineering and regenerative medicine strategies for the female breast

Obesity‐Associated Adipose Stromal Cells Promote Breast Cancer Invasion through Direct Cell Contact and ECM Remodeling

Assessment of human adipose‐derived stem cell on surface‐modified silicone implant to reduce capsular contracture formation

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