Adipose-Derived Stem Cells: Isolation, Characterization, and Differentiation Potential

Huang, Shyh Jer; Fu, Ru Huei; Shyu, Woei Cherng; Liu, Shih‐Ping; Jong, Gwo Ping; Chiu, Yung Wei; Wu, Hsiao Su; Tsou, Yung An; Cheng, Chao; Lin, Shinn Zong

doi:10.3727/096368912x655127

Cited by 122 publications

(98 citation statements)

References 99 publications

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“…The human adipose-derived stem cells (hADSCs) derived from the human adipose tissue can differentiate into other functional cells for reconstruction of the bone, cartilage, tendon, skeletal muscle, adipose, and other tissues in special conditions [4][5][6]. Early in 2001, it has been proved that hADSCs possess the potentiality to multidirectional differentiation by Zuk et al [7] and its directional differentiation into osteoblasts has been causing more attentions [8][9][10]. Due to its stronger proliferation in vitro, massive source and availability from the patients, less pain, and minimum immune exclusion [11][12][13], ADSCs are considered as an ideal seed cell in tissue engineering.…”

Section: Introductionmentioning

confidence: 98%

Dynamic Fabrication of Tissue-Engineered Bone Substitutes Based on Derived Cancellous Bone Scaffold in a Spinner Flask Bioreactor System

Song

Zhu

et al. 2014

Appl Biochem Biotechnol

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The in vitro dynamic fabrications of tissue-engineered bones were performed to assess the advantages of human adipose-derived stem cells (hADSCs) combined with acellular cancellous bone scaffold coming from fresh pig femur in a spinner flask compared with traditional static culture. In this study, the bio-derived cancellous bone was regarded as a biomimetic scaffold, and its surface appearance was observed under scanning electron microscopy (SEM). Moreover, its modulus of elasticity and chemical composition were measured with universal testing machine (UTM) and infrared detector, respectively. hADSCs were inoculated into cancellous bone scaffold at a density of 1 × 10(6) cells/mL and cultured in spinner flask and T-flask with osteogenic medium (OM) for 2 weeks, respectively. Following to this, the osteogenic differentiation was qualitatively and quantitatively detected with alkaline phosphatase (ALP) kits, and the cell growth and viability were assayed using Live/Dead staining; cell adhesion and extracellular matrix secretion were observed under a SEM. The average pore size of cancellous bone scaffold was 284.5 ± 83.62 μm, the elasticity modulus was 41.27 ± 15.63 MPa, and it also showed excellent biocompatibility. The hADSCs with multidifferentiation potentials were well proliferated, could grow to 90 % fusion within 5 days, and were therefore suitable to use as seed cells in the construction of tissue-engineered bones. After 2 weeks of fabrication, cells were well-distributed on scaffolds, and these scaffolds still remained intact. Compared to static environment, the ALP expression, cell distribution, and extracellular matrix secretion on cancellous bones in spinner flask were much better. It confirmed that three-dimensional dynamic culture in spinner flask promoted ADSC osteogenic differentiation, proliferation, and matrix secretion significantly to make for the fabrication of engineered bone substitutes.

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Section: Introductionmentioning

confidence: 98%

Dynamic Fabrication of Tissue-Engineered Bone Substitutes Based on Derived Cancellous Bone Scaffold in a Spinner Flask Bioreactor System

Song

Zhu

et al. 2014

Appl Biochem Biotechnol

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“…In addition, ADSCs have also been indicated to suppress inflammation, promote angiogenesis and enhance fibroblast proliferation (5,26). Human ADSCs isolated by using minimally invasive procedures could illustrate tremendous potential in tissue repair and tissue regeneration applications (13,16,40).…”

Section: Introductionmentioning

confidence: 99%

Human Adipose-Derived Stem Cells Accelerate the Restoration of Tensile Strength of Tendon and Alleviate the Progression of Rotator Cuff Injury in a Rat Model

Chen

Chan

et al. 2015

Cell Transplant

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Adult stem cell therapy for the treatment of tendon injuries is a growing area of research. This study is aimed to investigate the efficacy of human adipose-derived stem cell (hADSC) injection on the tendon during its healing process in a rat model of rotator cuff injury. hADSCs were injected 3 days after collagenase-induced rotator cuff injuries in experimental groups, while the control group received saline as a placebo. Histological and biomechanical analyses were performed 7, 14, 21, and 28 days after collagenase injection. Compared to the control group, it was found that inflammatory cells were significantly decreased in the hADSC-treated group after collagenase injection for 7 and 14 days. In the hADSC-injected group, the fiber arrangement and tendon organization had also been improved. On the seventh day after collagenase injection, the load to failure of the hADSC-injected group (15.87 ± 2.20 N) was notably higher than that of the saline-injected group (11.20 ± 1.35 N). It is suggested that the tensile strength of the supraspinatus tendon was significantly enhanced. Local administration of hADSCs might have the possibility to restore the tensile strength and attenuate the progression of tendinitis. Taken together, these findings demonstrate that the recovery processes in damaged tendons can be facilitated architecturally and functionally after hADSC injection.

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“…Human adipose tissueederived stromal cells (ASCs) share both structural markers and self-renewal, proliferation and differentiation capacities with mesenchymal stromal cells (MSCs), yielding multiple lineages in vitro, including adipocytes, osteoblasts, chondrocytes, myocytes and neuronal cells [1,2]. These cells bear therapeutic potential in regenerative medicine and tissue engineering applications [3e5].…”

Section: Introductionmentioning

confidence: 99%

Storage effect on viability and biofunctionality of human adipose tissue-derived stromal cells

2015

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Adipose-Derived Stem Cells: Isolation, Characterization, and Differentiation Potential

Cited by 122 publications

References 99 publications

Dynamic Fabrication of Tissue-Engineered Bone Substitutes Based on Derived Cancellous Bone Scaffold in a Spinner Flask Bioreactor System

Dynamic Fabrication of Tissue-Engineered Bone Substitutes Based on Derived Cancellous Bone Scaffold in a Spinner Flask Bioreactor System

Human Adipose-Derived Stem Cells Accelerate the Restoration of Tensile Strength of Tendon and Alleviate the Progression of Rotator Cuff Injury in a Rat Model

Storage effect on viability and biofunctionality of human adipose tissue-derived stromal cells

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