Adipose Stromal Vascular Fraction Isolation

Kim, Seong Kee

doi:10.1097/prs.0000000000000188

Cited by 5 publications

(4 citation statements)

References 1 publication

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“…This led to the exclusion of 12 studies[ 11 , 14 , 19 - 28 ] due to factors such as thematic inconsistency and methodological flaws, highlighting the critical evaluation process in maintaining the integrity of the review. Furthermore, the research designs of two additional studies[ 29 , 30 ] resulted in their exclusion, underscoring the stringent adherence to the review's methodological standards.…”

Section: Resultsmentioning

confidence: 99%

Non-enzymatic methods for isolation of stromal vascular fraction and adipose-derived stem cells: A systematic review

Mundluru,

Naidu,

Mundluru

et al. 2024

World J Methodol

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BACKGROUND Adipose-derived stem cells (ADSCs) and the stromal vascular fraction (SVF) have garnered substantial interest in regenerative medicine due to their potential to treat a wide range of conditions. Traditional enzymatic methods for isolating these cells face challenges such as high costs, lengthy processing time, and regu-latory complexities. AIM This systematic review aimed to assess the efficacy and practicality of non-enzymatic, mechanical methods for isolating SVF and ADSCs, comparing these to conventional enzymatic approaches. METHODS Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines, a comprehensive literature search was conducted across multiple databases. Studies were selected based on inclusion criteria focused on non-enzymatic isolation methods for SVF and ADSCs from adipose tissue. The risk of bias was assessed, and a qualitative synthesis of findings was performed due to the methodological heterogeneity of the included studies. RESULTS Nineteen studies met the inclusion criteria, highlighting various mechanical techniques such as centrifugation, vortexing, and ultrasonic cavitation. The review identified significant variability in cell yield and viability, and the integrity of isolated cells across different non-enzymatic methods compared to enzymatic procedures. Despite some advantages of mechanical methods, including reduced processing time and avoidance of enzymatic reagents, the evidence suggests a need for optimization to match the cell quality and therapeutic efficacy achievable with enzymatic isolation. CONCLUSION Non-enzymatic, mechanical methods offer a promising alternative to enzymatic isolation of SVF and ADSCs, potentially simplifying the isolation process and reducing regulatory hurdles. However, further research is necessary to standardize these techniques and ensure consistent, high-quality cell yields for clinical applications. The development of efficient, safe, and reproducible non-enzymatic isolation methods could significantly advance the field of regenerative medicine.

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Section: Resultsmentioning

confidence: 99%

Non-enzymatic methods for isolation of stromal vascular fraction and adipose-derived stem cells: A systematic review

Mundluru,

Naidu,

Mundluru

et al. 2024

World J Methodol

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“…Adipose SVF-based therapy holds great promise for treating several musculoskeletal disorders, including OA [4,17,31]. Gathering better knowledge of techniques for isolating the adipose SVF is demanding among experts for reinforcing traditional systems for OA care [24,26]. Biological insights on the healing potential of such approaches can be instrumental for clinicians to select the best therapeutic modality in handling various OA severity.…”

Section: Discussionmentioning

confidence: 99%

“…Achieving adipose SVF can envisage the use of both enzymatic and nonenzymatic methods; however, the lack of standardized protocols prevents clear-cut results on their performance [22,23]. Thanks to technological advances, new research perspectives have opened in this field with the generation of point-of-care (POC) systems [24][25][26].…”

Section: Introductionmentioning

confidence: 99%

“…Further preclinical studies are necessary to identify suitable POC systems for processing and preserving the biological features of SVF. Selecting the proper method is required to examine several aspects, including cellular survival, cell yield, presence of GMP facilities and, last but not least, the disease severity [24][25][26]. The main goal of this study was to assess in vitro the impact of the mechanical technique with Hy-Tissue SVF system, a class IIa CE marked device of adipose tissue micro-fragmentation, on the biological features and physiological functions of the generated adipose product (mSVF) with a direct comparison with the enzymatic SVF (eSVF).…”

Section: Introductionmentioning

confidence: 99%

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Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care

Desando

Bartolotti

Cattini

et al. 2021

Stem Cell Rev and Rep

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Gathering a better grasp on the adipose stromal vascular fraction (SVF) is demanding among clinicians for osteoarthritis (OA) care because of its promising but multifaceted clinical outcomes. The aim of this preclinical in vitro study was to test whether the mechanical approach with Hy-Tissue SVF system, a class IIa CE marked device of adipose tissue micro-fragmentation, influences the biological features and functions of SVF. We compared mechanical generated-SVF (mSVF) with the enzymatic generated-SVF (eSVF) by testing cell survival, phenotype, differentiation, and paracrine properties using ELISA assays. Both adipose SVF showed 80% viable cells and enrichment for CD-44 marker. The mSVF product preserved the functions of cell populations within the adipose tissue; however, it displayed lowered nucleated cell recovery and CFU-F than eSVF. As for multipotency, mSVF and eSVF showed similar differentiation commitment for osteochondral lineages. Both adipose SVF exhibited an increased release of VEGF, HGF, IGF-1 and PDGF-bb, involved in pathways mediating osteochondral repair and cell migration. Both mSVF and eSVF also displayed high release for the anti-inflammatory cytokine IL-10. After in vitro culture, supernatants from both mSVF and eSVF groups showed a low release of cytokines except for IL-10, thereby giving evidence of functional changes after culture expansion. In this study, mSVF showed active cell populations in the adipose tissue comparable to eSVF with excellent survival, differentiation and paracrine properties under a new mechanical adipose tissue micro-fragmentation system; thereby suggesting its potential use as a minimally invasive technique for OA treatment. Graphical abstract

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Comparison of intraoperative procedures for isolation of clinical grade stromal vascular fraction for regenerative purposes: a systematic review

Dongen

Tuin

Spiekman

et al. 2017

J Tissue Eng Regen Med

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Intraoperative application of the stromal vascular fraction (SVF) of adipose tissue requires a fast and efficient isolation procedure of adipose tissue. This review was performed to systematically assess and compare procedures currently used for the intraoperative isolation of cellular SVF (cSVF) and tissue SVF (tSVF) that still contain the extracellular matrix. Pubmed, EMBASE and the Cochrane central register of controlled trials databases were searched for studies that compare procedures for intraoperative isolation of SVF (searched 28 September 2016). Outcomes of interest were cell yield, viability of cells, composition of SVF, duration, cost and procedure characteristics. Procedures were subdivided into procedures resulting in a cSVF or tSVF. Thirteen out of 3038 studies, evaluating 18 intraoperative isolation procedures, were considered eligible. In general, cSVF and tSVF intraoperative isolation procedures had similar cell yield, cell viability and SVF composition compared to a nonintraoperative (i.e. culture laboratory-based collagenase protocol) control group within the same studies. The majority of intraoperative isolation procedures are less time consuming than nonintraoperative control groups, however. Intraoperative isolation procedures are less time-consuming than nonintraoperative control groups with similar cell yield, viability of cells and composition of SVF, and therefore more suitable for use in the clinic. Nevertheless, none of the intraoperative isolation procedures could be designated as the preferred procedure to isolate SVF.

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Adipose Stromal Vascular Fraction Isolation

Cited by 5 publications

References 1 publication

Non-enzymatic methods for isolation of stromal vascular fraction and adipose-derived stem cells: A systematic review

Non-enzymatic methods for isolation of stromal vascular fraction and adipose-derived stem cells: A systematic review

Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care

Comparison of intraoperative procedures for isolation of clinical grade stromal vascular fraction for regenerative purposes: a systematic review

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