Adipose tissue cysteine dioxygenase type 1 is associated with an anti-inflammatory profile, impacting on systemic metabolic traits

Ado-Mediated Synthesis of Taurine Alters the Chromatin Landscape of Inguinal Adipose Tissue to Enhance Non-Shivering Thermogenesis

“…The copyright holder for this this version posted February 3, 2023. ; https://doi.org/10.1101/2023.02.02.526843 doi: bioRxiv preprint flavoprotein subunit a (Sdha) [41,42]. Our study adds to field by providing the first evidence that the biosynthetic enzyme Ado is also critical for taurine synthesis.…”

“…Taurine biosynthesis can originate from two biological starting points, cysteine-driven synthesis through the Cdo enzyme or cysteamine-driven synthesis through the Ado enzyme. The enzyme Cdo has been well-studied and it has been shown that the loss-of-Cdo in adipocytes significantly reduces taurine levels and impairs mitochondrial function by blunting mitochondrial respiration as well as inhibiting mitochondrial gene expression, such as cytochrome c oxidase subunit 5b ( Cox5b ), ubiquinol-cytochrome c reductase core protein 1 ( Uqcrc1 ) and succinate dehydrogenase complex flavoprotein subunit a ( Sdha ) [41,42]. Our study adds to field by providing the first evidence that the biosynthetic enzyme Ado is also critical for taurine synthesis.…”

Adipose tissue coregulates cognitive function

“…Adipose tissue samples were obtained from SAT and VAT during elective surgical procedures (cholecystectomy, surgery of abdominal hernia, and gastric bypass surgery) ( 68 ). Both SAT and VAT samples were collected from the abdomen, following standard procedures.…”

“…VAT and SAT RNA purification was performed using RNeasy-Tissue Mini-Kit (QIAGEN). Total RNA was quantified by Qubit RNA BR Assay kit (Thermo Fisher Scientific), and the integrity was checked by using the RNA Kit (15NT) on 5300 Fragment Analyzer System (Agilent Technologies) ( 68 ).…”

Cysteine dioxygenase type 1: A new player in adipose physiology and metabolic health

“…investigated the CDO1 gene expression levels and their associations with systemic metabolic profiles in a large Caucasian population comprising of 4 independent cohorts (N Cohort 1 = 299; N Cohort 2 = 150; N Cohort 3 = 65; and N Cohort 4 = 30 individuals) with varying obesity levels (Body mass index/BMI Cohorts1–3 = 20–68 kg/m 2 ; BMI Cohort 4 >35 kg/m 2 ). 10 The clinical findings were further substantiated by performing a series of in vitro experiments involving a variety of cell types, such as isolated human adipocytes, human preadipocytes, and human immortalized adipose-derived mesenchymal stem cells (ACS52telo cells). Adipose samples from cohort 4 were also subjected to a high throughput RNAseq analysis.…”