Adipose Tissue-derived Stem Cells: The Friendly Side of a Classic Cardiovascular Foe

Sanz‐Ruiz, Ricardo; Santos, María Eugenia Fernández; Muñoa, Marta Domínguez; Martin, I; Parma, Radosław; Fernández, Pedro; Fernández‐Avilés, Francisco

doi:10.1007/s12265-007-9006-9

Cited by 14 publications

(9 citation statements)

References 45 publications

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“…Recently, stem-cell based therapies have been explored as a means for regeneration of heart tissue [4], with functional differentiation of implanted stem cells into mature cardiomyocytes [5], [6], [7], [8]. Thus far, different populations of adult stem cells have been examined following implantation in animal models of and humans with heart disease [9], [10], [11], [12], [13]. While the reported therapeutic benefits have been inconsistent, autologous cell implantation has generally been deemed safe.…”

Section: Introductionmentioning

confidence: 99%

Adult Cardiac Progenitor Cell Aggregates Exhibit Survival Benefit Both In Vitro and In Vivo

et al. 2012

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BackgroundA major hurdle in the use of exogenous stems cells for therapeutic regeneration of injured myocardium remains the poor survival of implanted cells. To date, the delivery of stem cells into myocardium has largely focused on implantation of cell suspensions.Methodology and Principal FindingsWe hypothesize that delivering progenitor cells in an aggregate form would serve to mimic the endogenous state with proper cell-cell contact, and may aid the survival of implanted cells. Microwell methodologies allow for the culture of homogenous 3D cell aggregates, thereby allowing cell-cell contact. In this study, we find that the culture of cardiac progenitor cells in a 3D cell aggregate augments cell survival and protects against cellular toxins and stressors, including hydrogen peroxide and anoxia/reoxygenation induced cell death. Moreover, using a murine model of cardiac ischemia-reperfusion injury, we find that delivery of cardiac progenitor cells in the form of 3D aggregates improved in vivo survival of implanted cells.ConclusionCollectively, our data support the notion that growth in 3D cellular systems and maintenance of cell-cell contact improves exogenous cell survival following delivery into myocardium. These approaches may serve as a strategy to improve cardiovascular cell-based therapies.

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Section: Introductionmentioning

confidence: 99%

Adult Cardiac Progenitor Cell Aggregates Exhibit Survival Benefit Both In Vitro and In Vivo

et al. 2012

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“…Studies have shown that ADSCs display similar immunoregulatory properties as human BMSC's by inhibiting the proliferation and cytokine secretion of human primary T cells in response to mitogens and allogenic T cells [49]. ADSCs have emerged as a new and promising type of stem cell with two distinct advantages over previously used adult stem cells: (i) easy and repeatable access allows harvesting of high amounts of adipose tissue, and (ii) ADSCs have an increased potential to proliferate and expand themselves in culture [16]. Low level laser irradiation can positively affect human ADSCs by increasing cellular proliferation, viability and protein expression [50,51] which highlights yet another potential advantage for the use of these cells in TE applications as the initial cell number could be increased before commencing differentiation leading to a higher yield of differentiated cells.…”

Section: Resultsmentioning

confidence: 99%

“…Cellular therapy has transpired as a potential new therapeutic option to treat cardiovascular disease [15]. ADSCs are able to differentiate into cardiomyocytes, hence these autologous adult stem cells are emerging as a new source of cells for cardiovascular repair [16]. Yamada et al (2006) [17] reported that CD29+ murine ADSCs from brown adipose tissue (BAT) could differentiate into cardiomyocytes with high efficiency.…”

Section: Cardiac Applicationsmentioning

confidence: 99%

Adipose Derived Stem Cells and Smooth Muscle Cells: Implications for Regenerative Medicine

Villiers

Houreld

Abrahamse

2009

Stem Cell Rev and Rep

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The treatment of chronic wounds and other damaged tissues and organs remains a difficult task, in spite of greater adherence to recognised standards of care and a better understanding of pathophysiologic principles. Adipose derived stem cells (ADSCs), with their proliferative and impressive differentiation potential, may be used in the future in autologous cell therapy or grafting to replace damaged tissues. At this point in time, transplanted tissues are often rejected by the body. Autologous grafting would eliminate this problem. ADSCs are able to differentiate into a number of cells in vitro, for example smooth muscle cells (SMCs), when treated with lineage specific factors. SMCs play a key role in physiology and pathology as they form the principle layer of all SMC tissues. Smooth muscle biopsies are often impractical and morbid, and often lead to a low cell harvest. It has also been shown that SMCs derived from a diseased organ can lead to abnormal cells. Therefore, there is a great need for alternative sources of healthy SMCs. The use of ADSCs for cell-based tissue engineering (TE) represents a promising alternative for smooth muscle repair. This review discusses the potential uses of ADSCs and SMCs in regenerative medicine, and the potential of ADSCs to be differentiated into functional SMCs for TE and regenerative cellular therapies to repair diseased organs.

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“…ADSCs represent a population of stem cells located in the adipose tissue that are able to differentiate into multiple cell lineages including cardiomyocytes and vascular cells [156] . They offer two major advantages over some previously mentioned types of cells: firstly, the easy and repeatable access that makes it possible to harvest large amounts of adipose tissue by a minimally invasive method and, secondly, their increased proliferative potential in culture [157] .…”

Section: Other Sources Of Stem Cellsmentioning

confidence: 99%

“Second-generation” stem cells for cardiac repair

García

Sanz‐Ruiz

Santos

et al. 2015

WJSC

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Over the last years, stem cell therapy has emerged as an inspiring alternative to restore cardiac function after myocardial infarction. A large body of evidence has been obtained in this field but there is no conclusive data on the efficacy of these treatments. Preclinical studies and early reports in humans have been encouraging and have fostered a rapid clinical translation, but positive results have not been uniformly observed and when present, they have been modest. Several types of stem cells, manufacturing methods and delivery routes have been tested in different clinical settings but direct comparison between them is challenging and hinders further research. Despite enormous achievements, major barriers have been found and many fundamental issues remain to be resolved. A better knowledge of the molecular mechanisms implicated in cardiac development and myocardial regeneration is critically needed to overcome some of these hurdles. Genetic and pharmacological priming together with the discovery of new sources of cells have led to a "second generation" of cell products that holds an encouraging promise in cardiovascular regenerative medicine. In this report, we review recent advances in this field focusing on the new types of stem cells that are currently being tested in human beings and on the novel strategies employed to boost cell performance in order to improve cardiac function and outcomes after myocardial infarction.

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Adipose Tissue-derived Stem Cells: The Friendly Side of a Classic Cardiovascular Foe

Cited by 14 publications

References 45 publications

Adult Cardiac Progenitor Cell Aggregates Exhibit Survival Benefit Both In Vitro and In Vivo

Adult Cardiac Progenitor Cell Aggregates Exhibit Survival Benefit Both In Vitro and In Vivo

Adipose Derived Stem Cells and Smooth Muscle Cells: Implications for Regenerative Medicine

“Second-generation” stem cells for cardiac repair

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