Adipose tissue is less responsive to food restriction anti-inflammatory effects than liver, muscle, and brain in mice

Antunes, Marina Masetto; Almeida-Souza, C.B. de; Godoy, Guilherme; Crisma, Amanda Rabello; Masi, Laureane Nunes; Curi, Rui; Bazotte, Roberto Barbosa

doi:10.1590/1414-431x20188150

Cited by 8 publications

(9 citation statements)

References 15 publications

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“…Healthy individuals undergoing short-term CR have been shown to have reduced monocyte metabolic and inflammatory activity and a drastic decrease in circulating and organ monocyte numbers [55,56]. The recruitment of inflammatory cells in tissues and airway induced by intake of high fat or high caloric diet could be reversed by CR [57,58]. In this study, our results established that CR could reduce the levels of pulmonary and circulating inflammatory triggers.…”

Section: Discussionsupporting

confidence: 65%

Caloric restriction attenuates C57BL/6J mouse lung injury and systemic toxicity induced by real ambient particulate matter exposure

Li¹,

Shen²,

Li³

et al. 2020

Preprint

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Background: Caloric restriction (CR) is known to improve health and extend life span in human beings. The effects of CR on adverse health outcomes in response to particulate matter (PM) exposure and the underlying mechanisms have yet to be defined. Results: Male C57BL/6J mice were fed with CR diet or ad libitum (AL) and exposed to PM for 4 weeks in a real-ambient PM exposure system located at Shijiazhuang, China, with a daily mean concentration (95.77 μg/m³) of PM 2.5. Compared to AL-fed mice, CR-fed mice attenuated PM-induced pulmonary injury and systemic toxicity characterized by reduction in oxidative stress, DNA damage and inflammation. Analysis of RNA sequencing revealed that several pulmonary pathways involved in production of ROS, cytokine production, and inflammatory cell activation were inactivated, while those mediating antioxidant generation and DNA repair were activated in CR-fed mice upon PM exposure. In addition, transcriptome analysis of murine livers revealed that CR led to induction of xenobiotic metabolism and detoxification pathways, corroborated by increased levels of urinary metabolites of polycyclic aromatic hydrocarbons (PAHs) and decreased cytotoxicity measured in an ex vivo assay. Conclusion: These novel results demonstrate, for the first time, that CR in mice confers resistance against pulmonary injuries and systemic toxicity induced by PM exposure. CR led to activation of xenobiotic metabolism and enhanced detoxification of PM-bound chemicals. These findings provide evidence that dietary intervention may afford therapeutic means to reduce the health risk associated with PM exposure. Keywords: Caloric restriction, particulate matter, pulmonary injury, systemic effects, RNA sequencing, xenobiotic metabolism.

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Section: Discussionsupporting

confidence: 65%

Caloric restriction attenuates C57BL/6J mouse lung injury and systemic toxicity induced by real ambient particulate matter exposure

Li¹,

Shen²,

Li³

et al. 2020

Preprint

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show abstract

“…Overnight-fasted mice (from 17:00 to 08:00), as described in our previous studies (9,(14)(15)(16)(17), were then euthanized by decapitation. Gastrocnemius muscle from both legs was removed, frozen in liquid nitrogen, and stored at -80°C until analysis.…”

Section: Animals and Experimental Designmentioning

confidence: 99%

“…Diet-induced obesity promotes insulin resistance (4,5), lipid accumulation (6,7), and inflammation (8,9) in skeletal muscle. The regulation of skeletal muscle FA composition is not fully understood; however, it markedly changes with dietary macronutrient composition.…”

Section: Introductionmentioning

confidence: 99%

A high-carbohydrate diet induces greater inflammation than a high-fat diet in mouse skeletal muscle

Antunes

Godoy

Almeida-Souza

et al. 2020

Braz J Med Biol Res

Self Cite

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We previously reported that both the high-carbohydrate diet (HCD) and high-fat diet (HFD) given for two months promote lipid deposition and inflammation in the liver and brain of mice. The results obtained indicate a tissue-specific response to both diets. Herein, we compared the effects of HCD and HFD on fatty acid (FA) composition and inflammation in the gastrocnemius muscle. Male Swiss mice were fed with HCD or HFD for 1 or 2 months. Saturated FA (SFA), monounsaturated FA (MUFA), n-3 polyunsaturated FA (n-3 PUFA), and n-6 PUFA were quantified. The activities of stearoyl-CoA desaturase 1 (SCD-1), D-6 desaturase (D6D), elongase 6, and de novo lipogenesis (DNL) were estimated. As for indicators of the inflammatory tissue state, we measured myeloperoxidase (MPO) activity and gene expression of F4/80, tumor necrosis factor-a (TNF-a), interleukin (IL)-4, IL-6, and IL-10. The HCD led to a lower deposition of SFA, MUFA, n-3 PUFA, and n-6 PUFA compared to HFD. However, the HCD increased arachidonic acid levels, SFA/n-3 PUFA ratio, DNL, SCD-1, D6D, and MPO activities, and expression of IL-6, contrasting with the general idea that increased lipid deposition is associated with more intense inflammation. The HCD was more potent to induce skeletal muscle inflammation than the HFD, regardless of the lower lipid accumulation.

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“…Healthy individuals undergoing short-term CR have been shown to have reduced monocyte metabolic and inflammatory activity and a drastic decrease in circulating and organ monocyte numbers [58,59]. The recruitment of inflammatory cells in tissues and airway induced by intake of high fat or high caloric diet could be reversed by CR [60,61]. In this study, we establish that CR could reduce the levels of pulmonary and circulating inflammatory triggers.…”

Section: Discussionmentioning

confidence: 56%

Caloric restriction attenuates C57BL/6 J mouse lung injury and extra-pulmonary toxicity induced by real ambient particulate matter exposure

Shen

et al. 2020

Part Fibre Toxicol

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Background: Caloric restriction (CR) is known to improve health and extend lifespan in human beings. The effects of CR on adverse health outcomes in response to particulate matter (PM) exposure and the underlying mechanisms have yet to be defined. Results: Male C57BL/6 J mice were fed with a CR diet or ad libitum (AL) and exposed to PM for 4 weeks in a realambient PM exposure system located at Shijiazhuang, China, with a daily mean concentration (95.77 μg/m 3) of PM 2.5. Compared to AL-fed mice, CR-fed mice showed attenuated PM-induced pulmonary injury and extrapulmonary toxicity characterized by reduction in oxidative stress, DNA damage and inflammation. RNA sequence analysis revealed that several pulmonary pathways that were involved in production of reactive oxygen species (ROS), cytokine production, and inflammatory cell activation were inactivated, while those mediating antioxidant generation and DNA repair were activated in CR-fed mice upon PM exposure. In addition, transcriptome analysis of murine livers revealed that CR led to induction of xenobiotic metabolism and detoxification pathways, corroborated by increased levels of urinary metabolites of polycyclic aromatic hydrocarbons (PAHs) and decreased cytotoxicity measured in an ex vivo assay. Conclusion: These novel results demonstrate, for the first time, that CR in mice confers resistance against pulmonary injuries and extra-pulmonary toxicity induced by PM exposure. CR led to activation of xenobiotic metabolism and enhanced detoxification of PM-bound chemicals. These findings provide evidence that dietary intervention may afford therapeutic means to reduce the health risk associated with PM exposure.

show abstract

Adipose tissue is less responsive to food restriction anti-inflammatory effects than liver, muscle, and brain in mice

Cited by 8 publications

References 15 publications

Caloric restriction attenuates C57BL/6J mouse lung injury and systemic toxicity induced by real ambient particulate matter exposure

Caloric restriction attenuates C57BL/6J mouse lung injury and systemic toxicity induced by real ambient particulate matter exposure

A high-carbohydrate diet induces greater inflammation than a high-fat diet in mouse skeletal muscle

Caloric restriction attenuates C57BL/6 J mouse lung injury and extra-pulmonary toxicity induced by real ambient particulate matter exposure

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