Adipose tissue miRNA level variation through conjugated linoleic acid supplementation in diet-induced obese rats

Abstract

Background. Conjugated linoleic acid (CLA), which is an octadecadienoic acid isomer, is believed to play different positive physiological roles, such as lowering body fat. Due to some reported side effects of CLA, like lipodystrophy and impaired glucose metabolism, it is important to establish its safety by understanding detailed molecular mechanisms. One of these mechanisms may be the role of this dietary agent in modifying the function and activity of microRNAs (miRNAs). Objectives. The aim of the study was to investigate how adipocyte miR-27a and miR-143 expression may be influenced by CLA in obese rats. Material and methods. In this study, 24 male Wistar rats were randomly divided into normal-fat diet (NFD) and high-fat diet (HFD) groups. After 8 weeks, the rats were weighed and half of the diet-induced obese rats were randomly selected to receive 500 mg CLA per 1 kg body weight for 4 weeks. At the end of this period, epididymal fat was isolated to investigate the expression level of miRNAs by real-time polymerase chain reaction (RT-PCR). Results. After 12 weeks, the obese rats in the HFD group, compared with rats in the NFD group, demonstrated a significant decrease in the expression of miR-27a (p < 0.05) and a significant increase in the expression of miR-143 (p < 0.05). In the group which had received CLA for a 4-week period, these events were reversed. Moreover, the rats in this group gained less weight than other rats in HFD groups, although the difference was not statistically significant. Conclusions. In conclusion, this study demonstrated that CLA, as an anti-obesity agent, may minimize abnormal changes in miRNA expression in obesity. This suggests a new pathway for weight loss; however, further studies are needed.

Chain length of dietary fatty acids determines gastrointestinal motility and visceromotor function in mice in a fatty acid binding protein 4-dependent manner

“…Concurrently, throughout the period of feeding, the cumulative food intake in both COCO and EPO groups was similar to STD, which provides additional evidence that supplementation with MCFAs, derived from coconut oil, or LCFAs, found in evening primrose oil, do not influence the appetite of animals. These findings are in accordance with other studies [ 52 – 54 ].…”

Evaluation of miRNA-143 and miRNA-145 Expression and Their Association with Vitamin-D Status Among Obese and Non-Obese Type-2 Diabetic Patients

“… 22 The HFD-induced overexpression of miRNA-143 in the adipose tissue done by RT-PCR. 25 In the context of T2DM macrovascular complications, these are linked with aberrant miRNA-145 expression to saphenous vein smooth muscle cell senescence. 26 Both miRNA-143 and miRNA-145 promote the transition of vascular smooth muscle cells to a dysfunctional phenotype associated with T2DM.…”

MicroRNA Modulation by Dietary Supplements in Obesity

“…These findings suggest that HFD might suppress miR-27 expression in adipose tissue, leading to the enhanced activity of several genes that promote adipocyte differentiation and fat accumulation. In light of this, dietary agents that are able to target miR-27, enhancing its expression, might effectively inhibit adipogenesis, promote weight loss, and improve metabolic alterations in obesity [50]. Similarly, Parra et al evaluated the effect of CLA treatment on microRNA expression in C57BL/6J mice.…”