Adjuvancy of Endotoxin from Heptoseless <i>Salmonella</i> Mutants

Nakano, Masayasu; Shimamura, Tadakatsu; Saito, Kazuhisa

doi:10.1111/j.1348-0421.1971.tb00557.x

Cited by 5 publications

(2 citation statements)

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“…Endotoxic glycolipid extracted from heptoseless mutants (Re form) of Salmonella, which consists only of lipid and 2-keto-3-deoxyoctonate (KDO) without the polysaccharide, still retained adjuvant activity for the antibody response when it was injected together with adequate doses of antigen [16]. Lipid A as obtained from the glycolipid or LPS after hydrolysis with mild acid is biologically inactive, while lipid A solubilized by complexing with bovine serum albumin (BSA) can recover adjuvant activity [2,15].…”

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Immunosuppressive Effect of Bacterial Lipopolysaccharide on Antibody Response

Nakano

Tanabe

Saito

et al. 1976

Japanese Journal of Microbiology

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Injection of endotoxins (bacterial lipopolysaccharide: LPS) several days prior to immunization causes the suppression of antibody response. The suppressive effects of several kinds of LPS preparations on the plaque-forming cell (PFC) antibody response in the spleen of mice were examined after immunization with sheep red blood cells (SRBC). Glycolipids obtained from heptoseless mutants (Re form) of salmonella or its lipid A preparation coupled artificially with bovine serum albumin (BSA) are capable, like LPS obtained from a wild type (S form) strain, of inducing suppression of the PFC response, while alkaline-detoxified LPS can not. The refractory periods of the PFC response induced by LPS injection last only a few days. However, the use of cyclophosphamide (CY) together with LPS can extend the refractory periods of antigenic stimulation for several weeks. Injections of LPS and CY can also induce unresponsive states of OH agglutinin antibody response to antigenic stimulation with formalin-killed organisms of Escherichia coli or Salmonella enteritidis (presumably both thymusindependent antigens). These unresponsive states induced by LPS and CY are easily terminated by a transfer of syngeneic bone marrow cells but not by thymocyte transfer.

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Immunosuppressive Effect of Bacterial Lipopolysaccharide on Antibody Response

Nakano

Tanabe

Saito

et al. 1976

Japanese Journal of Microbiology

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“…lipid A as an endotoxic component and polysaccharide or protein as a carrier, are necessary for the expression of its endotoxic activity. The adjuvant-active part of LPS seems, also, to be in the lipid A component [3,14], but the precise role of the carrier portion of the LPS in the expression of in vivo adjuvanticity has not been clarified.…”

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confidence: 99%