Adjuvant effect of Bacillus firmus on the expression of cytokines and toll-like receptors in mouse nasopharynx-associated lymphoid tissue (NALT) after intranasal immunization with inactivated influenza virus type A

Zanvit, Peter; Tichopád, Aleš; Havlíčková, Martina; Novotná, Oľga; Jirkovská, Marie; Kološtová, Katarína; Cechová, D; Julák, Jaroslav; Šterzl, I; Prokešová, L.

doi:10.1016/j.imlet.2010.08.006

Cited by 10 publications

(5 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To determine the nature and intensity of the inflammatory response to the implant covered with carp collagen and compare it to that induced by bovine collagen covered implant, we chose albino mice BALB/c strain, which has been previously shown to be responsive to collagens [22, 23]. We measured the serum level of proinflammatory and anti-inflammatory cytokines (IL-1 β , IL-4, IL-10, IL-13, TNF- α , and IFN- γ ), their secretion by in vitro stimulated splenocytes, proliferating activity of in vitro stimulated splenocytes, and local production of selected cytokine (TNF- α , IL-6, IL-10, TGF- β ) and chemokine (CCL2 and CCL3) mRNA as previously done by our group and others [11, 13].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of the Immunogenicity of a Vascular Graft Covered with Collagen Derived from the European Carp (Cyprinus carpio) and Bovine Collagen

Lambert

Nováková

Cechová

et al. 2019

BioMed Research International

View full text Add to dashboard Cite

Aim. To assess the systemic and local immunological response to subcutaneous implants of a vascular graft covered with collagen extracted from the European carp (freshwater fish) or with collagen of bovine origin. Methods. Pieces of a vascular graft covered by pure bovine (Bos taurus, BOV, n=14) or carp (Cyprinus carpio, CYP, n=14) collagen 5 mm in size were implanted subcutaneously in the dorsum of a Balb/cOla mice. A sham operation group of 12 animals served as the control. At 7 and 14 days after the operation, one-half of each group was terminated and blood for serum, spleen, and implant with surrounding tissue were collected. Mean cytokine (TNF-α, IL-10, IL-4, IL-1β, IL-13, and IFN-γ) levels in serum were determined using ELISA. Spleen cell cultures were used for in vitro testing of lymphocyte proliferation and cytokine secretion. Local expressions of IL-6, IL-10, TNF-α, TGF-β, CCL-2, and CCL-3 were determined using PCR. Results. We found no significant difference among control, BOV, and CYP groups in mean cytokine serum levels at seven days. At day 14, the BOV group had higher levels of TNF-α (P=.018) and both the BOV and CYP groups had lower levels of IL-4 (P=.011 and P=.047, respectively) compared with the control group. Both tested implants showed only a minimal effect on the production of selected cytokines. Cell proliferation in the CYP group stimulated by CYP gel at 14 days was significantly lower than by BOV gel in BOV group (P=.0031) or by CYP gel in the control group (P=.041). The difference between the groups in the local RNA expression of all the tested mediators both at 7 and at 14 days was not significant apart from a lower level of TNF-α in the BOV group compared to CYP at 14 days (P=.013). Conclusions. Implants covered with carp collagen induce an immunological response that is comparable to that of bovine collagen covered implants in a mouse model.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The production of proinflammatory and regulatory cytokines correlates with the intensity of the inflammatory response. Therefore, it can be used as a marker of its severity and extent [11, 13].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Immunogenicity of a Vascular Graft Covered with Collagen Derived from the European Carp (Cyprinus carpio) and Bovine Collagen

Lambert

Nováková

Cechová

et al. 2019

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…The total RNA concentration was estimated by spectrophotometric measurements at 260 nm assuming that 40 µg of RNA per mililitre equal one absorbance unit. Real time PCR was performed as described previously [23]. Briefly, RNA was converted to cDNA using Taq-Man reverse transcription reagents (Applied Biosystems, Foster City, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Lysate of Probiotic Lactobacillus casei DN-114 001 Ameliorates Colitis by Strengthening the Gut Barrier Function and Changing the Gut Microenvironment

et al. 2011

View full text Add to dashboard Cite

BackgroundProbiotic bacteria can be used for the prevention and treatment of human inflammatory diseases including inflammatory bowel diseases (IBD). However, the nature of active components and exact mechanisms of this beneficial effects have not been fully elucidated. Our aim was to investigate if lysate of probiotic bacterium L. casei DN-114 001 (Lc) could decrease the severity of intestinal inflammation in a murine model of IBD.Methodology/Principal FindingsThe preventive effect of oral administration of Lc significantly reduces the severity of acute dextran sulfate sodium (DSS) colitis in BALB/c but not in SCID mice. In order to analyze how this beneficial effect interferes with well-known phases of intestinal inflammation pathogenesis in vivo and in vitro, we evaluated intestinal permeability using the FITC-labeled dextran method and analysed tight junction proteins expression by immunofluorescence and PCR. We also measured CD4+FoxP3+ regulatory T cells proportion by FACS analysis, microbiota composition by pyrosequencing, and local cytokine production by ELISA. Lc leads to a significant protection against increased intestinal permeability and barrier dysfunction shown by preserved ZO-1 expression. We found that the Lc treatment increases the numbers of CD4+FoxP3+ regulatory T cells in mesenteric lymph nodes (MLN), decreases production of pro-inflammatory cytokines TNF-α and IFN-γ, and anti-inflammatory IL-10 in Peyer's patches and large intestine, and changes the gut microbiota composition. Moreover, Lc treatment prevents lipopolysaccharide-induced TNF-α expression in RAW 264.7 cell line by down-regulating the NF-κB signaling pathway.Conclusion/SignificanceOur study provided evidence that even non-living probiotic bacteria can prevent the development of severe forms of intestinal inflammation by strengthening the integrity of intestinal barrier and modulation of gut microenvironment.

show abstract

“…In the experiments on vaccination against P. aeruginosa infection the intranasal route was only as effective as the intramuscular route when cholera toxin was used as an adjuvant [69]. Bacillus firmus had an adjuvant effect via the expression of cytokines [70]. An expansion of DC in the nose was induced by the use of P6 with outer membrane protein and alpha-galactosylceramide in nontypeable Haemophilus influenza [71].…”

Section: Adjuvants In Intranasal Vaccinationmentioning

confidence: 99%

Mucosal vaccination by the intranasal route. Nose-associated lymphoid tissue (NALT)—Structure, function and species differences

Pabst¹

2015

Vaccine

View full text Add to dashboard Cite

Adjuvant effect of Bacillus firmus on the expression of cytokines and toll-like receptors in mouse nasopharynx-associated lymphoid tissue (NALT) after intranasal immunization with inactivated influenza virus type A

Cited by 10 publications

References 29 publications

Evaluation of the Immunogenicity of a Vascular Graft Covered with Collagen Derived from the European Carp (Cyprinus carpio) and Bovine Collagen

Evaluation of the Immunogenicity of a Vascular Graft Covered with Collagen Derived from the European Carp (Cyprinus carpio) and Bovine Collagen

Lysate of Probiotic Lactobacillus casei DN-114 001 Ameliorates Colitis by Strengthening the Gut Barrier Function and Changing the Gut Microenvironment

Mucosal vaccination by the intranasal route. Nose-associated lymphoid tissue (NALT)—Structure, function and species differences

Contact Info

Product

Resources

About