Adjuvant effect of Bordetella pertussis vaccine to sheep erythrocytes: enhancement of antibody formation by using subcutaneous administration of adjuvant and antigen

Abstract

The subcutaneous route (s.c.) was used to study the adjuvant effect of Bordetella pertussis vaccine (PV) on the primary antibody response to sheep erythrocytes. The reasons for using the s.c. route are discussed. PV, besides enhancing the hemagglutinin response, also markedly increased the number of plaqueforming cells in the draining lymph nodes. A heated preparation of PV was tested and found to possess significant adjuvant activity. Interestingly, the enhancement occurred in the absence of marked enlargement of the lymph nodes, which was characteristic of the unheated preparation. In addition, a crude solubilized cell-free preparation of PV was tested and also found to possess significant adjuvant activity. The activity was only partially abolished by heat.Hence, it was concluded that both heat-labile as well as heat-stable factors contributed to the adjuvanticity of PV. The studies also support the view that the draining lymph nodes represent a principal locus of action of PV and that the s.c. route of administration of adjuvant and antigen provides a suitable model for studying and assaying the adjuvanticity of PV.

Immune suppression and induction of gamma interferon by pertussis toxin

“…The results in Table 3 are representative of three series of experiments and show that higher interferon activity was consistently observed in Lytl +depleted cultures, whereas Lyt2+ depletion resulted in lowered interferon activity. DISCUSSION B. pertussis cells have been reported to enhance antibody responsiveness to a variety of unrelated antigens (5,13,30). However, the best adjuvant effect is obtained when B. pertussis is injected simultaneously with antigen (5,30).…”

“…DISCUSSION B. pertussis cells have been reported to enhance antibody responsiveness to a variety of unrelated antigens (5,13,30). However, the best adjuvant effect is obtained when B. pertussis is injected simultaneously with antigen (5,30). If administered a few days before or after antigen, B. pertussis may actually suppress the response.…”

Adjuvant effect of Bordetella pertussis vaccine to sheep erythrocytes in mice: enhancement of cell-mediated immunity by subcutaneous administration of adjuvant and antigen

“…As with PV, the adjuvanticity of PS was completely eliminated by heat. DISCUSSION Whereas the enhancing effect of PV on the humoral antibody response to SRBC has been extensively studied (3,5,8,18,19), the ability of PV to enhance the CMI response to SRBC has received virtually no attention. In the present report, the adjuvant effect of PV on the CMI response to SRBC was studied.…”

“…However, the role of CMI in tumor rejection and inhibition of viral replication in these investigations was not substantiated. In a previous publication (19), we reported on an in vivo model of studying the adjuvant effect of PV on the antibody response to sheep erythrocytes (SRBC) in which we used exclusively the subcutaneous (s.c.) route of administration of adjuvant and antigen. Also, we have reported (18) on the adjuvant effect of PV on the in vitro antibody response to SRBC with normal spleen cells, studied through the Mishell-Dutton immunization technique.…”

“…PV. Fluid B. pertussis (lots 6WE 37A and 7BE 85A), purchased from Eli Lilly & Co., Indianapolis, Ind., contained approximately 2.6 x 1010 to 2.9 x 1010 organisms per ml, as determined by methods previously described (19).…”