Adjuvant effects of therapeutic glycolipids administered to a cohort of NKT cell-diverse pigs

Artiaga, Bianca Libanori; Whitener, Robert L.; Staples, C.R.; Driver, John P.

doi:10.1016/j.vetimm.2014.09.006

Cited by 31 publications

(48 citation statements)

References 55 publications

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“…The iNKT cell adjuvant effects in pigs to intramuscular delivered a-GalCer with hen-egg lysozyme showed increased frequency of iNKT cells in circulation with enhanced Th1 and Th2 cytokines response (Artiaga et al, 2014). In intranasally vaccinated pigs, we found mucosal adjuvant effects of a-GalCer to inactivated SwIV Ags, indicated by enhanced innate and adaptive cytokine responses in the lungs.…”

Section: Discussionmentioning

confidence: 64%

“…Like humans, pig is a natural host for all three subtypes of influenza H1N1, H1N2, and H3N2 viruses. Pig has iNKT cell and CD1d transcripts, and recently iNKT cell in pig was discovered and its adjuvant effects to a model Ag was demonstrated using a-GalCer (Artiaga et al, 2014;Eguchi-Ogawa et al, 2007;Looringh van Beeck et al, 2009;Renukaradhya et al, 2010). Further, pigs genetically knocked out for CD1d were shown to be iNKT cell deficient (Yang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Adjuvant effects of invariant NKT cell ligand potentiates the innate and adaptive immunity to an inactivated H1N1 swine influenza virus vaccine in pigs

Dwivedi

Manickam

Dhakal

et al. 2016

Veterinary Microbiology

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Section: Discussionmentioning

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Adjuvant effects of invariant NKT cell ligand potentiates the innate and adaptive immunity to an inactivated H1N1 swine influenza virus vaccine in pigs

Dwivedi

Manickam

Dhakal

et al. 2016

Veterinary Microbiology

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“…Conversely, when mouse-adapted influenza viruses are used, inbred mice usually develop much more severe disease compared to pigs and humans as many strains express a functionally inactive form of the antiviral protein Mx11718. Mice also have drawbacks for modeling NKT-cell immunotherapy because murine NKT-cell frequencies, subsets and tissue localization patterns differ considerably compared to pigs and humans15162526.…”

Section: Discussionmentioning

confidence: 99%

“…and dripped into the nostrils (i.n.). Stock solutions of α-GalCer were prepared in DMSO as previously described15. Control pigs were housed separately from CA04 animals.…”

Section: Methodsmentioning

confidence: 99%

“…Once activated, NKT-cells stimulate a diverse array of innate and adaptive immune responses important for controlling a wide range of infectious diseases14. We recently demonstrated that swine co-injected with the NKT-cell superagonist, α-galactosylceramide (α-GalCer), and a model antigen hen-egg lysozyme (HEL) generate strong antigen-specific T cell and antibody responses15. We also demonstrated that intramuscular (i.m.)…”

mentioning

confidence: 99%

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Rapid control of pandemic H1N1 influenza by targeting NKT-cells

Artiaga

Yang

Hutchinson

et al. 2016

Sci Rep

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Swine influenza A viruses (IAV) are a major cause of respiratory disease in pigs and humans. Currently approved anti-influenza therapies directly target the virus, but these approaches are losing effectiveness as new viral strains quickly develop drug resistance. To over come this challenge, there is an urgent need for more effective antiviral drugs. Here we tested the anti-influenza efficacy of the invariant natural killer T (NKT) cell superagonist, α-galactosylceramide (α-GalCer), which stimulates a wide array of anti-viral immune responses. We show that intranasal but not systemic administration of α-GalCer to piglets infected with pandemic A/California/04/2009 (CA04) H1N1 IAV ameliorated disease symptoms and resulted in the restoration of weight gain to the level of uninfected pigs. Correspondingly, viral titers in the upper-and lower-respiratory tract were reduced only in piglets that had received intranasal α-GalCer. Most significantly, lung inflammation as a consequence of virus persistence was largely prevented when NKT-cells were targeted via the respiratory route. Thus, targeting mucosal NKT-cells may provide a novel and potent platform for improving the course of disease in swine infected with seasonal and pandemic influenza viruses, and leads to the suggestion that this may also be true in humans and therefore deserves further study.

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Development of Plasmodium‐specific liver‐resident memory CD8⁺ T cells after heat‐killed sporozoite immunization in mice

et al. 2021

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Malaria remains a major cause of mortality in the world and an efficient vaccine is the best chance of reducing the disease burden. Vaccination strategies for the liver stage of disease that utilise injection of live radiation‐attenuated sporozoites (RAS) confer sterile immunity, which is mediated by CD8+ memory T cells, with liver‐resident memory T cells (TRM) being particularly important. We have previously described a TCR transgenic mouse, termed PbT‐I, where all CD8+ T cells recognize a specific peptide from Plasmodium. PbT‐I form liver TRM cells upon RAS injection and are capable of protecting mice against challenge infection. Here, we utilize this transgenic system to examine whether nonliving sporozoites, killed by heat treatment (HKS), could trigger the development of Plasmodium‐specific liver TRM cells. We found that HKS vaccination induced the formation of memory CD8+ T cells in the spleen and liver, and importantly, liver TRM cells were fewer in number than that induced by RAS. Crucially, we showed the number of TRM cells was significantly higher when HKS were combined with the glycolipid α‐galactosylceramide as an adjuvant. In the future, this work could lead to development of an antimalaria vaccination strategy that does not require live sporozoites, providing greater utility.

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Adjuvant effects of therapeutic glycolipids administered to a cohort of NKT cell-diverse pigs

Cited by 31 publications

References 55 publications

Adjuvant effects of invariant NKT cell ligand potentiates the innate and adaptive immunity to an inactivated H1N1 swine influenza virus vaccine in pigs

Adjuvant effects of invariant NKT cell ligand potentiates the innate and adaptive immunity to an inactivated H1N1 swine influenza virus vaccine in pigs

Rapid control of pandemic H1N1 influenza by targeting NKT-cells

Development of Plasmodium‐specific liver‐resident memory CD8⁺ T cells after heat‐killed sporozoite immunization in mice

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Adjuvant effects of therapeutic glycolipids administered to a cohort of NKT cell-diverse pigs

Cited by 31 publications

References 55 publications

Adjuvant effects of invariant NKT cell ligand potentiates the innate and adaptive immunity to an inactivated H1N1 swine influenza virus vaccine in pigs

Adjuvant effects of invariant NKT cell ligand potentiates the innate and adaptive immunity to an inactivated H1N1 swine influenza virus vaccine in pigs

Rapid control of pandemic H1N1 influenza by targeting NKT-cells

Development of Plasmodium‐specific liver‐resident memory CD8+ T cells after heat‐killed sporozoite immunization in mice

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Development of Plasmodium‐specific liver‐resident memory CD8⁺ T cells after heat‐killed sporozoite immunization in mice