Adjuvant is the Major Parameter Influencing the Isotype Profiles Generated During Immunization with a Protein Antigen, the Schistosoma mansoni Sm28‐GST

Abstract

We have previously shown that immunization of mice with the vaccine candidate, the 28-kDa glutathione-S-transferase of Schistosoma mansoni (Sm28-GST), in alum or complete Freund's adjuvant, or with recombinant Salmonella typhimurium expressing Sm28-GST, induced type 2, mixed, or type 1 immune responses, respectively. In the present study we examined whether the genetic background, the dose and the route of antigen administration could modulate the profile of the immune response induced during these immunizations. Our results show that the nature of the adjuvant is the major factor that determines the profile of the response. Surprisingly, the genetic background did not influence the response, while the route of immunization, and to a lesser extent the dose of the antigen, weakly modulated the adjuvant-dependent orientation of the immune response.

Human and Murine Humoral Immune Recognition of Multiple Peptides from Schistosoma mansoni Glyceraldehyde 3‐P Dehydrogenase is Associated with Resistance to Schistosomiasis

“…The present data did not support the results of Comoy et al . [17] who reported that the nature of the adjuvant is the major factor that determines the isotype profiles generated during immunization with Sm28‐GST, whereas the genetic background did not modulate the response. Nevertheless, no SG3PDH‐specific IgG3, IgA or IgE were detected in any individual mouse of the three strains.…”

Immunogenicity of self-adjuvanticity oral vaccine candidate based on use of Bacillus subtilis spore displaying Schistosoma japonicum 26 KDa GST protein

“…This suggested that the mucosal antibody response would not be sustained for very long after vaccination, although immunological memory may ensure a rapid mucosal response to the vaccine antigen upon subsequent re-exposure. As the relative amounts of serum IgG1 and IgG2a antibodies closely matched IL-4 and IFN-gamma cytokine profiles, respectively, it is considered that IgG1 and IgG2a antibody responses are good markers for Th1 and Th2 cytokine expression (Comoy et al 1998). The present study has confirmed that IgG1 and IgG2a were produced at approximately equal levels following mucosal immunization, which indicates a mixed Th1/Th2 cytokine response in our spore-based model of vaccination using SjGST antigen.…”

Expression of a 28-Kilodalton Glutathione S -Transferase Antigen of Schistosoma mansoni on the Surface of Filamentous Phages and Evaluation of Its Vaccine Potential

“…The pattern of antibody responses to GST differs significantly, depending on the type of adjuvant used (6). For example, when aluminum hydroxide was used as an adjuvant to immunize mice with Sm28GST, a strong IgG1 response was obtained, whereas fusion of Sm28GST with tetanus toxoid resulted in a pronounced IgG2a response (6).…”

“…For example, when aluminum hydroxide was used as an adjuvant to immunize mice with Sm28GST, a strong IgG1 response was obtained, whereas fusion of Sm28GST with tetanus toxoid resulted in a pronounced IgG2a response (6). Similarly, immunization with Sm28GST in complete Freund's adjuvant resulted in high levels of both IgG1 and IgG2a (7).…”