Adjuvant L-arginine treatment in controlled ovarian hyperstimulation: a double-blind, randomized study

Battaglia, Cesare; Regnani, Giorgia; Marsella, Tiziana; Facchinetti, Fabio; Volpe, Annibale; Venturoli, Stefano; Flamigni, Carlo

doi:10.1093/humrep/17.3.659

Cited by 51 publications

(40 citation statements)

References 30 publications

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“…While NO produced in low concentration would act as cytoprotective factor [30], NO overproduction could interfere with mitochondrial respiration and result in significant damage to cellular components and cellular death [31]. Also in ovaries, although follicular fluid NO derivatives are most likely to be necessary for oocyte activation at fertilization when produced within physiological limits, at higher doses they can have detrimental consequences on embryo quality, implantation and pregnancy rate [32].…”

Section: Surico Et Al: Antioxidant Effects Of Hcg In Endothelial Cellsmentioning

confidence: 99%

Human Chorionic Gonadotropin Protects Vascular Endothelial Cells from Oxidative Stress by Apoptosis Inhibition, Cell Survival Signalling Activation and Mitochondrial Function Protection

Surico¹,

Farruggio

Marotta

et al. 2015

Cell Physiol Biochem

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Background/Aim: Previous reports have made it hypothetically possible that human chorionic gonadotropin (hCG) could protect against the onset of pregnancy-related pathological conditions by acting as an antioxidant. In the present study we planned to examine the effects of hCG against oxidative stress in human umbilical vein endothelial cells (HUVEC). Methods: HUVEC were subjected to peroxidation by hydrogen peroxide. The modulation of nitric oxide (NO) release by hCG and its effects on cell viability, glutathione (GSH) levels, mitochondrial membrane potential and mitochondrial transition pore opening (MPTP) were examined by specific dyes. Endothelial and inducible NO synthase (eNOS and iNOS), Akt and extracellular -signal-regulated kinases 1/2 (ERK1/2) activation and markers of apoptosis were analyzed by Western Blot. Results: In HUVEC, hCG reduced NO release by modulating eNOS and iNOS. Moreover, hCG protected HUVEC against oxidative stress by preventing GSH reduction and apoptosis, by maintaining Akt and ERK1/2 activation and by keeping mitochondrial function. Conclusion: The present results have for the first time shown protective effects exerted by hCG on vascular endothelial function, which would be achieved by modulation of NO release, antioxidant and antiapoptotic actions and activation of cell survival signalling. These findings could have clinical implications in the management of pregnancy-related disorders.

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Section: Surico Et Al: Antioxidant Effects Of Hcg In Endothelial Cellsmentioning

confidence: 99%

Human Chorionic Gonadotropin Protects Vascular Endothelial Cells from Oxidative Stress by Apoptosis Inhibition, Cell Survival Signalling Activation and Mitochondrial Function Protection

Surico¹,

Farruggio

Marotta

et al. 2015

Cell Physiol Biochem

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“…Apart from the above functions, NO may be directly involved in the physiology of reproductive organs. The NO participates in periovulatory modulation of ovarian blood flow (4)(5)(6) and seems to be involved in follicular maturation and ovulation (1,7). However, although the NO local synthesis within the uterus may be important for regulating endometrial, cervical, and myometrial activity (8), its role in embryonic implantation has not been fully explored.…”

Section: Introductionmentioning

confidence: 99%

Embryonic Production of Nitric Oxide and Its Role in Implantation: A Pilot Study

Battaglia

Ciotti

Notarangelo

et al. 2003

J Assist Reprod Genet

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Purpose : To investigate the ability of human embryos to produce nitric oxide (NO) and correlate its production with embryo quality and pregnancy rate. Methods : Twenty-three women participated in the study and were submitted to controlled ovarian stimulation and intracytoplasmic sperm injection. Embryos were singularly cultured in medium microdrops of 50 µL and were replaced, by transcervical transfer, at the 2-to 6-cell stage. In the culture media of each embryo the NO production was assessed by monitoring the levels of its stable oxidation products (nitrites/nitrates). Results : All the 23 patients underwent embryo transfer. After microinjection 64 embryos were obtained. The mean number of transferred embryos was 2.61 ± 0.46 and the pregnancy rate was 26%. The mean nitrite/nitrate concentrations of culture medium of each embryo was significantly higher (5.88 ± 2.34 µmol/L) than in pure P-1 medium (0.81 ± 0.21 µmol/L; p < 0.001) demonstrating an embryonic secretion of NO. Comparing pregnant (7.34 ± 2.72 µmol/L) versus nonpregnant patients (5.53 ± 1.49 µmol/L; p = 0.022), the mean nitrite/nitrate concentrations were significantly higher. Furthermore, the best quality embryos of pregnant women produced significantly higher nitrite/nitrate concentrations than those of not pregnant patients. Conclusions : It seems that NO production in nidating embryos is increased and that it may be primarily associated with a better morphology and a better growth potential of developing embryos.

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“…Although Manau et al could not find any correlation between NO and embryo quality, most of the studies have concluded that the NO concentrations in FFs were negatively correlated with embryo quality, similar with our study results. [20][21][22][23] The oocyte protein synthesis is kept under control by maternal mRNA along 48 h following fertilization till the third division of the embryo. The subsequent process is under control of embryonic genome.…”

Section: Discussionmentioning

confidence: 99%

Effect of Follicular Fluid Oxidative Stress on Subsequent Oocyte Quality and Embryo Development

Bige¹,

Gülekli²,

Demir³

et al. 2016

Turkiye Klinikleri J Gynecol Obst

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n vitro fertilization, an important assisted reproduction technique (ART), is widely used in infertility clinics all over the world. The sucess of this technique is strongly associated with oocyte and embryo quality. Thus biochemical characteristics of follicular fluid surrounding oocytes play an important role in oocyte maturation and subsequent embryo quality. were assessed. The relationship between FF levels of oxidative stress markers [malondialdehyde (MDA), nitric oxide (NO) and protein carbonyl] levels and subsequent oocyte quality, fertilization and embryo morphologies were evaluated. R Re es su ul lt ts s: : The mean MDA levels were significantly higher in FFs of fertilized oocytes. The mean NO levels were significantly lower in FFs of top-quality embryos whereas mean protein carbonyl levels were significantly higher in FFs of top quality embryos. C Co on nc cl lu us si io on n: : FF oxidative stress seems to play an important role in fertilization and embryo morphology. Different oxidative stres pathways might have different effects on oocyte development, and also particular oxidative stress in FF might be needed for oocyte maturation.

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Adjuvant L-arginine treatment in controlled ovarian hyperstimulation: a double-blind, randomized study

Abstract: L-Arginine supplementation may be detrimental to embryo quality and pregnancy rate during controlled ovarian hyperstimulation cycles.

Cited by 51 publications

References 30 publications

Human Chorionic Gonadotropin Protects Vascular Endothelial Cells from Oxidative Stress by Apoptosis Inhibition, Cell Survival Signalling Activation and Mitochondrial Function Protection

Human Chorionic Gonadotropin Protects Vascular Endothelial Cells from Oxidative Stress by Apoptosis Inhibition, Cell Survival Signalling Activation and Mitochondrial Function Protection

Embryonic Production of Nitric Oxide and Its Role in Implantation: A Pilot Study

Effect of Follicular Fluid Oxidative Stress on Subsequent Oocyte Quality and Embryo Development

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