Adjuvant properties of polymer based on acrylic acid

Kozak, М. R.

doi:10.15407/ubj85.03.069

Cited by 6 publications

(4 citation statements)

References 12 publications

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“…2). The immunization of mice only with antigen (ovalbumin or BSA) without adjuvant did not cause specific antibody production [26].…”

Section: Resultsmentioning

confidence: 95%

Comparison of adjuvant properties of chitosan during oral and subcutaneous immunization of mice with BSA

Kozak¹,

Petruh²,

Влизло³

2022

Ukr.Biochem.J.

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Vaccination is the best method to prevent the spread of infectious diseases, its disadvantages are side effects. Potentially safe DNA, RNA or protein molecules possess antigenic properties, but are low-immunogenic and therefore require conjugation with an adjuvant. The aim of the research was to evaluate Chitosan (CS) potency as an adjuvant and compare its effectiveness depending on the route of drug administration. The experiments were carried out on 3 groups of BALB/c mice. Mice of the first group were injected subcutaneously with 20 µl of a mixture of CS (3.3 mg/kg) and BSA (1.7 mg/kg). The mixture of CS and BSA at the same doses and volume was administered orally to mice of the second experimental group. The third group – control – unvaccinated mice. Anti-BSA antibody levels were measured by ELISA. Aspartate aminotransferase, alanine aminotransferase activity and cholesterol, creatinine and urea levels were determined in the serum. It was found that both subcutaneous and mucosal immunizations provided a 2-fold increase in anti-BSA antibody titers against the background of maintaining all biochemical blood parameters at the level of the physiological norm. However, AST activity in the serum of oral-immunized mice was elevated as compared to subcutaneous-immunized mice. Serum cholesterol level in the group of subcutaneously immunized mice and creatinine and urea levels in both experimental groups were reduced compared to the control. It is concluded that oral immunization with CS is the optimal route for antigen-specific IgG antibody response induction.

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“…2). The immunization of mice only with antigen (ovalbumin or BSA) without adjuvant did not cause specific antibody production [26].…”

Section: Resultsmentioning

confidence: 95%

Comparison of adjuvant properties of chitosan during oral and subcutaneous immunization of mice with BSA

Kozak¹,

Petruh²,

Влизло³

2022

Ukr.Biochem.J.

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“…Hydrodynamic diameter of particles (from the research of the DLS) was 0.64 μm and Z-potential -49 mV. In our previous studies, we established the adjuvant properties of polymer of acrylic acid [6]. Adjuvant properties of the polymer were compared to aluminum hydroxide, which is a component of many traditional vaccines.…”

Section: Resultsmentioning

confidence: 99%

Histomorphology of organs and activity of some enzymes in mice after immunization with polymer based on acrylic acid or aluminum hydroxide as adjuvants

Kozak¹,

Martyn²,

OLIYNYK³

et al. 2018

Bìol. Tvarin

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Vaccination is the best biomedical approach in avoiding diseases. Proteins and peptides purified from microorganisms or synthesized chemically are weakly antigenic and need adjuvant to provide strong immune responses. Many substances with adjuvant properties have been discovered, however, only aluminum compounds stay traditionally in clinical use. The aim of this study was to evaluate biological influence on mice after immunization with a polymer of acrylic acid with adjuvant properties. The polymer was synthesized on the basis of glycidyl methacrylate, acrylic acid, triethylene glycol methacrylate and butyl acrylate. Histomorphology of the liver, kidney and spleen of white mice after subcutaneous administration of polymer or aluminum hydroxide was investigated. It has been established that the polymer and aluminum hydroxide did not cause changes in parenchymal organs of white mice. The structure of organs was preserved, no pathological changes were revealed. The activities of antioxidant enzymes-superoxide dismutase (SOD), catalase and glutation reductase (GP) at the application of adjuvants were studied. Immunization of mice with aluminum hydroxide showed an increase in the activity of SOD in the kidneys by 1.5 times (P<0.01), catalase in liver on 8 % (P<0.05), and GP in kidney on 30.6 % (P<0.001). Polymer based on acrylic acid caused a decrease of SOD activity in the liver by 1.78 times (P<0.01), and an increase of catalase activity on 10.5 % (P<0.05). At the same time, the activity of antioxidant enzymes in the spleen and kidneys of animals after the injection of the polymer did not differ from the control group.

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“…Penggunaan adjuvan polimer menghasilkan nilai OD lebih tinggi, dibandingkan lipofektamin yang merupakan pembawa DNA atau mRNA baku internasional (Kozak et al, 2013;Pardi et al, 2018;Rose et al, 2018;Shakya dan Nandakumar, 2023). Laporan penelitian sebelumnya dengan lipofektamin menghasilkan nilai OD tertinggi sebesar 0,452 (Mahardika et al, 2023).…”

Section: Hasil Dan Pembahasanunclassified

Respons Imun Mencit terhadap Vaksin DNA Virus Demam Babi Afrika A224L dan A276R dengan Enkapsulasi Lipofektamin, Kolesterol dan Polimer

Kamil,

Mahardika,

Suardana

2024

juvet

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A vaccine against African Swine Fever (ASF) is urgently needed to reduce losses for pig farmers throughout the world. The study was aimed to test the immune response of mice to an ASF-DNA vaccine with the A224L and A276R genes with polymer, lipofectamine, and cholesterol adjuvants. Each mouse was vaccinated intramuscularly with 25 μg of plasmid encapsulated with the above adjuvants. Mouse sera were collected at weeks 1, 2, 3 and 4. Antibodies against A224L and A276R were detected by ELISA test using synthetic peptides as antigens. Optical density (OD) values were analysed statistically using the Analysis of Variance test with the SPSS Version 25. The results showed that the OD values of the serum of mice given the ASF-A224L and A276R DNA vaccines with polymer adjuvants (0.149), lipofectamine (0.080), and cholesterol (0.058), as well as control mice (0.020) differed significantly (p= 0.000), while the time of serum collection had no significant effect (p=0.517). The conclusion is that the OD values of mice sera after DNA-ASF-A224L and A276R encapsulated with polymer, lipofectamine and cholesterol were statistically significant, while sampling time did not show any statistically difference. Further studies are needed to improve the adjuvant mixing protocol with larger quantities of plasmids.Keywords:mice; DNA vaccine; ASF; polymer; ELISA

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Adjuvant properties of polymer based on acrylic acid

Cited by 6 publications

References 12 publications

Comparison of adjuvant properties of chitosan during oral and subcutaneous immunization of mice with BSA

Comparison of adjuvant properties of chitosan during oral and subcutaneous immunization of mice with BSA

Histomorphology of organs and activity of some enzymes in mice after immunization with polymer based on acrylic acid or aluminum hydroxide as adjuvants

Respons Imun Mencit terhadap Vaksin DNA Virus Demam Babi Afrika A224L dan A276R dengan Enkapsulasi Lipofektamin, Kolesterol dan Polimer

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