Adjuvanticity of epimedium polysaccharide-propolis flavone on inactivated vaccines against AI and ND virus

Fan, Yunpeng; Wang, Deyun; Liu, Jiaguo; Hu, Yuanliang; Zhao, Xiaojuan; Han, Gangwen; Nguyen, The Luong; Chang, Shanshan

doi:10.1016/j.ijbiomac.2012.08.025

Cited by 18 publications

(14 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These HI antibody titres of chickens of group A is mostly similar to HI antibody titre of the chickens of group C (Fan et al, 2012;Iqbal et al, 2003 andRajeswar andMasillamoni, 2002).…”

Section: Discussionsupporting

confidence: 67%

Biological characterization and determination of comparative efficacy of an inactivated Newcastle disease virus vaccine prepared from velogenic strain

Hossain¹,

Azad²,

Akter³

et al. 2014

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

“…These HI antibody titres of chickens of group A is mostly similar to HI antibody titre of the chickens of group C (Fan et al, 2012;Iqbal et al, 2003 andRajeswar andMasillamoni, 2002).…”

Section: Discussionsupporting

confidence: 67%

Biological characterization and determination of comparative efficacy of an inactivated Newcastle disease virus vaccine prepared from velogenic strain

Hossain¹,

Azad²,

Akter³

et al. 2014

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

“…Immuno-regulation is an important pathway for individual resistance to viruses (Fan et al. 2012 ; Chen et al. 2015b ; Dabaghian et al.…”

Section: Discussionmentioning

confidence: 99%

“…Peripheral T lymphocytes were prepared according to the method of Fan et al. ( 2012 ). The blood sample was collected from a non-immunized 30-day-old cherry valley duck and immediately diluted by quadruple D-Hank’s containing sodium heparin.…”

Section: Methodsmentioning

confidence: 99%

Anti-DHAV-1 reproduction and immuno-regulatory effects of a flavonoid prescription on duck virus hepatitis

Chen

Zeng

Yang

et al. 2017

Pharmaceutical Biology

Self Cite

View full text Add to dashboard Cite

Context: The flavonoid prescription baicalin-linarin-icariin-notoginsenoside R1 (BLIN) has a curative effect on duck virus hepatitis (DVH) caused by duck hepatitis A virus type 1 (DHAV-1). However, the mechanism of this curative effect is not understood.Objective: This study investigates the mechanism of the curative effect of BLIN on DVH caused by DHAV-1. We analyzed the anti-DHAV-1 reproduction mechanism and immuno-regulatory effect of BLIN.Materials and methods: The anti-DHAV-1 reproduction effects of BLIN at 20, 10, 5 and 2.5 μg/mL in vitro, as well as the influence of BLIN at 20 μg/mL on DHAV-1 adsorption, replication and release were tested using the qRT-PCR method. The promotion abilities of BLIN at 20, 10, 5 and 2.5 μg/mL on T- and B-lymphocyte proliferation were investigated by the MTT method. IL-2 and IFN-γ levels and total anti-DHAV-1 antibody secretion after treatment with DHAV-1 for 4, 8 and 54 h were determined by ELISA.Results: BLIN showed a dose-dependent DHAV-1 reproduction inhibitory effect. The inhibitory effect was highest at 20 μg/mL, where DHAV-1 adsorption and release were significantly lower. Meanwhile, BLIN at 5 μg/mL significantly increased T and B lymphocyte proliferation. BLIN stimulated total anti-DHAV-1 antibody secretion in ducklings at the dosage of 4 mg per duckling, but did not stimulate IL-2 and IFN-γ secretion significantly.Conclusions: BLIN inhibits DHAV-1 reproduction by suppressing its adsorption and release. Additionally, BLIN promoted the duckling antiviral response.

show abstract

“…Peripheral T lymphocyte was prepared according to the method of Fan et al [16]. The blood sample was collected from a nonimmunized cherry valley duck at 30-day-old and transferred into test tubes immediately which containing sodium heparin and D-Hank's.…”

Section: Peripheral T Lymphocytesmentioning

confidence: 99%

“…Then, drugs at series of concentrations were added, 100 L per well and five wells each concentration. The 96-well culture plates containing T lymphocytes and drugs were divided into two parts [16]: one part with PHA control was added 20 L per well with PHA except for what treated as T lymphocytes control; another part only contained such control. Each well of the two parts was added to 200 L with RPMI-1640.…”

Section: Peripheral T Lymphocyte Proliferation Assaymentioning

confidence: 99%

Effects of Bush Sophora Root polysaccharide and its sulfate on immuno-enhancing of the therapeutic DVH

Chen

Xian

et al. 2015

International Journal of Biological Macromolecules

Self Cite

View full text Add to dashboard Cite

Adjuvanticity of epimedium polysaccharide-propolis flavone on inactivated vaccines against AI and ND virus

Cited by 18 publications

References 9 publications

Biological characterization and determination of comparative efficacy of an inactivated Newcastle disease virus vaccine prepared from velogenic strain

Biological characterization and determination of comparative efficacy of an inactivated Newcastle disease virus vaccine prepared from velogenic strain

Anti-DHAV-1 reproduction and immuno-regulatory effects of a flavonoid prescription on duck virus hepatitis

Effects of Bush Sophora Root polysaccharide and its sulfate on immuno-enhancing of the therapeutic DVH

Contact Info

Product

Resources

About