Administration and use of aquaculture drugs in Korea

Kim, Jin Woo; Young, Cho， Mi; Jee, Bo-Young; Park, Myoung Ae; Kim, Na Young

doi:10.7847/jfp.2014.27.1.067

Cited by 20 publications

(16 citation statements)

References 2 publications

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“…In this study, resistance to chloramphenicol, ciprofloxacin and ofloxacin were observed in several Aeromonas isolates which have been banned for the treatment of aquatic animal diseases in Korea (Kim et al . ).…”

Section: Resultsmentioning

confidence: 97%

Incidence of antimicrobial resistance genes and class 1 integron gene cassettes in multidrug-resistant motile Aeromonas sp. isolated from ornamental guppy (Poecilia reticulata)

Hossain

Silva

Wickramanayake

et al. 2019

Letters in Applied Microbiology

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Aeromonas sp. are opportunistic pathogenic bacteria which are associated with various diseases in ornamental fish, aquaculture raised species and wild fisheries. In our study, antimicrobial resistance patterns, antimicrobial resistance genes and class 1 integron gene cassettes of 52 guppy‐borne Aeromonas sp. were examined. The isolates were identified as A. veronii (n = 34), A. dhakensis (n = 10), A. hydrophila (n = 3), A. caviae (n = 3) and A. enteropelogenes (n = 2) by gyrB gene sequencing. Every isolate was resistant to at least four antimicrobials in disc diffusion test. The resistance to amoxicillin, nalidixic acid and oxytetracycline was 100% among the tested isolates. 92·30, 76·92, 71·15, 51·92, 51·92 and 50·00% of the isolates were resistant to ampicillin, rifampicin, imipenem, cephalothin, tetracycline and trimethoprim respectively. The multiple antibiotic resistance index values ranged from 0·28 to 0·67. PCR amplification of antimicrobial resistance genes implied the occurrence of tetracycline resistance (tetA (65·39%), tetE (25·00%) and tetB (15·38%)), plasmid‐mediated quinolone resistance (qnrS (26·92%) and qnrB (17·31%)) and aminoglycoside resistance (aphaAI‐IAB (7·69%) and aac (6′)‐Ib (3·84%)) genes in the isolates. The IntI gene was positive for 36·54% of the isolates and four class 1 integron gene cassette profiles (aadA2, qacE2‐orfD, aadA2‐catB2 and dfrA12‐aadA2) were identified. These data suggest that ornamental guppy can be a reservoir of multidrug‐resistant Aeromonas sp. which comprise different antimicrobial resistance genes and class 1 integrons. Significance and Impact of the Study Antimicrobial resistance genes and integron gene cassettes of ornamental fish‐borne aeromonads are poorly studied. The antimicrobial resistance patterns, antimicrobial resistance genes and class 1 integron gene cassettes of Aeromonas sp. isolated from ornamental guppy were characterized for the first time in Korea. The incidence of different antimicrobial resistance genes and class 1 integron gene cassettes were observed in multidrug‐resistant Aeromonas isolates. This result suggests that better management practices are necessary to prevent and address the serious consequences of indiscriminate and inappropriate antimicrobial use, and the distribution of multidrug‐resistant bacteria.

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Section: Resultsmentioning

confidence: 97%

Incidence of antimicrobial resistance genes and class 1 integron gene cassettes in multidrug-resistant motile Aeromonas sp. isolated from ornamental guppy (Poecilia reticulata)

Hossain

Silva

Wickramanayake

et al. 2019

Letters in Applied Microbiology

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“…; Kim et al . ). However, antimicrobial residues in the fish farming area are also responsible for the bacterial resistance (Kang et al .…”

Section: Resultsmentioning

confidence: 97%

“…Over the years, antimicrobials have been used to prevent bacterial diseases in aquaculture. The presumptive diagnosis and treatment of fish diseases are common due to the lack of trustworthy antimicrobial resistance data especially in the ornamental fish industry as there are a few antimicrobials legalized for controlling the aquatic animal diseases in Korean aquaculture (Kim et al 2014). Thus, the study about the emergence of antimicrobial resistance and resistance genes in zebrafish-borne Aeromonas spp.…”

Section: Resultsmentioning

confidence: 99%

Multidrug resistant Aeromonas spp. isolated from zebrafish (Danio rerio): antibiogram, antimicrobial resistance genes and class 1 integron gene cassettes

Hossain

Dahanayake

Silva

et al. 2019

Letters in Applied Microbiology

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Aeromonas spp. are Gram‐negative opportunistic bacteria which have been commonly associated with fish diseases. In this study, antibiogram, antimicrobial resistance genes and integrons of 43 zebrafish‐borne Aeromonas spp. were studied. The isolates were identified as six Aeromonas species (A. veronii biovar veronii (n = 26), A. veronii biovar sobria (n = 3), A. hydrophila (n = 8), A. caviae (n = 3), A. enteropelogenes (n = 2) and A. dhakensis (n = 1)). Antibiogram of the isolates indicated that most of them were resistant to amoxicillin (100·00%), nalidixic acid (100·00%), oxytetracycline (100·00%), ampicillin (93·02%), tetracycline (74·42%), rifampicin (67·44%) and imipenem (65·15%). Multiple antimicrobial resistance (MAR) index values ranged from 0·19–0·44 to 90·70% isolates showed multidrug resistance. PCR of antimicrobial resistance genes revealed that the tetracycline resistance gene (tetA) was the most predominant (67·44%) among the isolates. The qnrS (53·49%), tetB (30·23%), tetE (30·23%), qnrB (23·26%) and aac(6’)‐Ib‐cr (4·65%) genes were also detected. Class 1 integrase (IntI1) gene was found in 46·51% of the isolates. Two types of class 1 integron gene cassette profiles (qacG‐aadA6‐qacG and drfA1) were identified. The results showed that zebrafish‐borne aeromonads can harbour different types of antimicrobial resistance genes and class 1 integrons. Significance and Impact of the Study Aeromonas spp. are important pathogens found in diverse environments. Antimicrobial resistance genes and integrons of ornamental fish‐borne Aeromonas spp. are not well studied. The antibiogram, antimicrobial resistance genes and class 1 integrons of Aeromonas spp. isolated from zebrafish were characterized for the first time in Korea. The prevalence of tetracycline resistance genes, plasmid‐mediated quinolone resistance genes and class 1 integron gene cassettes were observed among the isolates. The qacG‐aadA6‐qacG gene cassette was identified for the first time in Aeromonas spp. The results suggest that the wise use of antimicrobials is necessary for the better management of the ornamental fish.

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“…National Fisheries Research and Development Institute (NFRDI) approved a list of permissive aquaculture antibiotics including AMP in Korea (Kim et al . ). The AMP resistance of V. parahaemolyticus isolates could be caused by overuse of antibiotics in aquaculture (IFT, ).…”

Section: Resultsmentioning

confidence: 97%

Occurrence of pathogenic Vibrio parahaemolyticus in seafood distribution channels and their antibiotic resistance profiles in S. Korea

Lee

Choi

Lee

et al. 2019

Lett Appl Microbiol

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This study investigated the occurrence of Vibrio parahaemolyticus in seafood distribution channels, and the incidence of pathogenic and antibiotic‐resistant strains. One hundred sixty‐seven samples (126 fishery samples and 41 environmental samples) were collected from fishery auction markets, fish markets and online markets in S. Korea from March to September, 2017. The samples were enriched in polymyxin broth and plated on CHROM agar Vibrio to isolate typical colonies. These were analysed by PCR to identify V. parahaemolyticus strains containing the hns gene and to examine the presence of pathogenic genes (tdh and trh). The isolates were screened for antibiotic susceptibility (amoxycillin/clavulanic acid, ampicillin (AMP), ampicillin/sulbactam, chloramphenicol, ciprofloxacin, erythromycin, gentamicin and tetracycline). Twenty‐eight samples (16·8%) were V. parahaemolyticus‐positive, and from the positive samples, three isolates (SMFM201708‐OY1, SMFM201709‐CO2‐2 and SMFM201709‐CO1‐2) were pathogenic which contained thermostable direct haemolysin or thermostable direct‐related haemolysin. These are the virulence factors associated with gastrointestinal symptoms. These three isolates were also resistant to AMP. These results indicate that there is high incidence of V. parahaemolyticus in seafood distribution channels, and three of the isolates were pathogenic, and the pathogenic strains showed AMP resistance. Therefore, food safety measures are necessary in a seafood distribution channel. Significance and Impact of the Study This study provides evidence for the incidence of pathogenic Vibrio parahaemolyticus in seafood distribution channels, especially for a fishery auction market, which showed the highest contamination and is a first place for a seafood distribution channel. Thus, V. parahaemolyticus in a fishery auction market may be cross‐contaminated during distribution, underlying the need of food safety regulation to control this pathogen in fishery auction market. Therefore, food safety regulation for fishery auction market should be established to control V. parahaemolyticus.

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Administration and use of aquaculture drugs in Korea

Cited by 20 publications

References 2 publications

Incidence of antimicrobial resistance genes and class 1 integron gene cassettes in multidrug-resistant motile Aeromonas sp. isolated from ornamental guppy (Poecilia reticulata)

Incidence of antimicrobial resistance genes and class 1 integron gene cassettes in multidrug-resistant motile Aeromonas sp. isolated from ornamental guppy (Poecilia reticulata)

Multidrug resistant Aeromonas spp. isolated from zebrafish (Danio rerio): antibiogram, antimicrobial resistance genes and class 1 integron gene cassettes

Occurrence of pathogenic Vibrio parahaemolyticus in seafood distribution channels and their antibiotic resistance profiles in S. Korea

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