Administration of docosahexaenoic acid before birth and until aging decreases kainate-induced seizures in adult zebrafish

Sierra, Saleta; Alfaro, Juan Manuel Esquivel; Sanchez, Sheralyn

doi:10.1016/j.brainresbull.2012.04.007

Cited by 17 publications

(12 citation statements)

References 24 publications

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“…PTZ was utilized as a proconvulsant for many adult zebrafish studies in this review (Pineda et al, 2011 , 2013 ; Banote et al, 2013 ; Mussulini et al, 2013 ; Siebel et al, 2013 ; Gupta et al, 2014 ; Cho et al, 2017 ; Duy et al, 2017 ; Kundap et al, 2017 ). Other types of proconvulsants such as KA (Sierra et al, 2012 ) were used to a lesser extent. Proconvulsants are typically administered through the oral, intraperitoneal (Sierra et al, 2012 ; Banote et al, 2013 ; Kundap et al, 2017 ) and bath application (Pineda et al, 2011 , 2013 ; Mussulini et al, 2013 ; Siebel et al, 2013 ; Gupta et al, 2014 ; Cho et al, 2017 ) routes.…”

Section: Resultsmentioning

confidence: 99%

“…Other types of proconvulsants such as KA (Sierra et al, 2012 ) were used to a lesser extent. Proconvulsants are typically administered through the oral, intraperitoneal (Sierra et al, 2012 ; Banote et al, 2013 ; Kundap et al, 2017 ) and bath application (Pineda et al, 2011 , 2013 ; Mussulini et al, 2013 ; Siebel et al, 2013 ; Gupta et al, 2014 ; Cho et al, 2017 ) routes. Each route of administration produces a similar behavioral response, with different seizure onset times at different concentrations.…”

Section: Resultsmentioning

confidence: 99%

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A Systematic Review on Non-mammalian Models in Epilepsy Research

et al. 2018

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Epilepsy is a common neurological disorder characterized by seizures which result in distinctive neurobiological and behavioral impairments. Not much is known about the causes of epilepsy, making it difficult to devise an effective cure for epilepsy. Moreover, clinical studies involving epileptogenesis and ictogenesis cannot be conducted in humans due to ethical reasons. As a result, animal models play a crucial role in the replication of epileptic seizures. In recent years, non-mammalian models have been given a primary focus in epilepsy research due to their advantages. This systematic review aims to summarize the importance of non-mammalian models in epilepsy research, such as in the screening of anti-convulsive compounds. The reason for this review is to integrate currently available information on the use and importance of non-mammalian models in epilepsy testing to aid in the planning of future studies as well as to provide an overview of the current state of this field. A PRISMA model was utilized and PubMed, Springer, ScienceDirect and SCOPUS were searched for articles published between January 2007 and November 2017. Fifty-one articles were finalized based on the inclusion/exclusion criteria and were discussed in this review. The results of this review demonstrated the current use of non-mammalian models in epilepsy research and reaffirmed their potential to supplement the typical rodent models of epilepsy in future research into both epileptogenesis and the treatment of epilepsy. This review also revealed a preference for zebrafish and fruit flies in lieu of other non-mammalian models, which is a shortcoming that should be corrected in future studies due to the great potential of these underutilized animal models.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

A Systematic Review on Non-mammalian Models in Epilepsy Research

et al. 2018

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“…Kynurenic acid is an excitatory amino acid receptor antagonist and potentially has protective effects 23 . DHA is the most abundant polyunsaturated acid in the brain and is involved in brain development 24 . DHA has recently been suggested as an interventional treatment of seizures in humans as it exerts antiepileptic effects in rodent models.…”

Section: Discussionmentioning

confidence: 99%

Exploratory Metabolomics Profiling in the Kainic Acid Rat Model Reveals Depletion of 25-Hydroxyvitamin D3 during Epileptogenesis

Heischmann

Quinn

Cruickshank‐Quinn

et al. 2016

Sci Rep

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Currently, no reliable markers are available to evaluate the epileptogenic potential of a brain injury. The electroencephalogram is the standard method of diagnosis of epilepsy; however, it is not used to predict the risk of developing epilepsy. Biomarkers that indicate an individual’s risk to develop epilepsy, especially those measurable in the periphery are urgently needed. Temporal lobe epilepsy (TLE), the most common form of acquired epilepsy, is characterized by spontaneous recurrent seizures following brain injury and a seizure-free “latent” period. Elucidation of mechanisms at play during epilepsy development (epileptogenesis) in animal models of TLE could enable the identification of predictive biomarkers. Our pilot study using liquid chromatography-mass spectrometry metabolomics analysis revealed changes (p-value ≤ 0.05, ≥1.5-fold change) in lipid, purine, and sterol metabolism in rat plasma and hippocampus during epileptogenesis and chronic epilepsy in the kainic acid model of TLE. Notably, disease development was associated with dysregulation of vitamin D3 metabolism at all stages and plasma 25-hydroxyvitamin D3 depletion in the acute and latent phase of injury-induced epileptogenesis. These data suggest that plasma VD3 metabolites reflect the severity of an epileptogenic insult and that a panel of plasma VD3 metabolites may be able to serve as a marker of epileptogenesis.

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“…Since the mortality rate of 10 mM PTZ induced-seizure were similar to 6 mg/kg KA injection [14] and lower than 15 mM PTZ in adult zebrafish, we decided to use 10 mM PTZ concentration to test all parameters of seizure severity, such as, score curve profile, and latency to reach score 4. These parameters were also tested, when fish are pretreated with a classical anticonvulsant, DZP, to test this behavioral tools for future use in AED research.…”

Section: Discussionmentioning

confidence: 99%

“…In this context, the zebrafish ( Danio rerio ) emerges as a new animal model to evaluate the effects of classical pro-convulsant drugs in order to develop and characterize new AED [10]–[14]. This species exhibits several anatomic similarities and a high genetic homology with mice and humans [15], [16].…”

Section: Introductionmentioning

confidence: 99%

Seizures Induced by Pentylenetetrazole in the Adult Zebrafish: A Detailed Behavioral Characterization

et al. 2013

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Pentylenetetrazole (PTZ) is a common convulsant agent used in animal models to investigate the mechanisms of seizures. Although adult zebrafish have been recently used to study epileptic seizures, a thorough characterization of the PTZ-induced seizures in this animal model is missing. The goal of this study was to perform a detailed temporal behavior profile characterization of PTZ-induced seizure in adult zebrafish. The behavioral profile during 20 min of PTZ immersion (5, 7.5, 10, and 15 mM) was characterized by stages defined as scores: (0) short swim, (1) increased swimming activity and high frequency of opercular movement, (2) erratic movements, (3) circular movements, (4) clonic seizure-like behavior, (5) fall to the bottom of the tank and tonic seizure-like behavior, (6) death. Animals exposed to distinct PTZ concentrations presented different seizure profiles, intensities and latencies to reach all scores. Only animals immersed into 15 mM PTZ showed an increased time to return to the normal behavior (score 0), after exposure. Total mortality rate at 10 and 15 mM were 33% and 50%, respectively. Considering all behavioral parameters, 5, 7.5, 10, and 15 mM PTZ, induced seizures with low, intermediate, and high severity, respectively. Pretreatment with diazepam (DZP) significantly attenuated seizure severity. Finally, the brain PTZ levels in adult zebrafish immersed into the chemoconvulsant solution at 5 and 10 mM were comparable to those described for the rodent model, with a peak after a 20-min of exposure. The PTZ brain levels observed after 2.5-min PTZ exposure and after 60-min removal from exposure were similar. Altogether, our results showed a detailed temporal behavioral characterization of a PTZ epileptic seizure model in adult zebrafish. These behavioral analyses and the simple method for PTZ quantification could be considered as important tools for future investigations and translational research.

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Administration of docosahexaenoic acid before birth and until aging decreases kainate-induced seizures in adult zebrafish

Cited by 17 publications

References 24 publications

A Systematic Review on Non-mammalian Models in Epilepsy Research

A Systematic Review on Non-mammalian Models in Epilepsy Research

Exploratory Metabolomics Profiling in the Kainic Acid Rat Model Reveals Depletion of 25-Hydroxyvitamin D3 during Epileptogenesis

Seizures Induced by Pentylenetetrazole in the Adult Zebrafish: A Detailed Behavioral Characterization

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