Administration of Human Chorionic Gonadotropin but Not of Luteinizing Hormone-Releasing Hormone at Pro-Oestrus or Late Di-Oestrus Has a Deleterious Effect on Pregnancy in the Rat

Mattheij, J.A.M.; Boer, P. de; Dekker, Bernadette L; Rijnders, E.M.; Swarts, J.J.M.

doi:10.1159/000298896

Cited by 8 publications

(15 citation statements)

References 13 publications

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“…Contrary to results obtained earlier [5,6], corpora lutea counts in hCG-treated rats were somewhat, al though not significantly, lower. The number of morpho logically normal morulae was intermediate between the normal pattern at day 3 [5] and the numbers implanting (table 1).…”

Section: Preimplantation Embryo Development In the Ratcontrasting

confidence: 99%

“…As in the rat [5], advancement of ovulation did not induce extra embryonic death in the mouse (compare LHRH with control). However, hCG-induced ovulation was followed by a considerable decrease of decidual reac tions and living fetuses at midterm.…”

Section: Embryo Survival At Days 12-14 O F Gestationmentioning

confidence: 78%

“…Embryonic losses mainly occurred before implantation [5], The effect proved to be dosedependent, a dose of 20 IU hCG almost halving the embryonic survival rate, whereas 80 IU led to 100% preimplantation loss and 5 IU had no effect. Up to between 45 and 69 h after the hCG injection, its delete rious effect could be counteracted by the injection of an anti-hCG monoclonal antibody [6], In the present study, we have extended our observations in three ways.…”

Section: Introductionmentioning

confidence: 94%

“…have been described extensively before [5,6]. A 10-hour dark period commenced at 5.00 p.m. Estrus cycles were followed by daily vaginal lavage.…”

Section: Ratsmentioning

confidence: 99%

“…When an analysis of Figures in parentheses are number of females. a Results from Mattheij et al [5]. b Results from Boerjan and de Boer [8], Table 2.…”

Section: Preimplantation Embryo Development In the Ratmentioning

confidence: 99%

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Embryo Loss, Blastomere Development and Chromosome Constitution after Human Chorionic Gonadotropin-Induced Ovulation in Mice and Rats with Regular Cycles

Boer¹,

Hoeven²,

Wolters³

et al. 1991

Gynecol Obstet Invest

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A dose of 7 IU human chorionic gonadotropin (hCG) given 14 h before the expected LH peak on proestrus significantly increased embryonic mortality in Swiss random-bred female mice to 5 5% of the number of corpora lutea. The use of luteinizing hormone-releasing hormone in a similar injection protocol did not induce embryonic death. The effect found in Swiss random-bred mice resembles that of a dose of 20 IU hCG in the rat. Afternoon-day-4 mouse embryos contained 39.1 ± 12.6 nuclei after hCG-induced ovulation compared to 46.2 ± 16.6 nuclei after spontaneous ovulation. For early-day-5 embryos of the rat, these figures were 34.2 ± 10.1 and 31.7 ± 8.4, respectively (mating was early on day 1). Numerical chromosome errors were estimated in secondary oocytes of the mouse and early-day-5 embryos of the rat. Compared with data from the literature, hCG seems to induce some extra meiotic nondisjunction in the rat only. Combining all genetic and physiological data, the loss of fecundity after hCG-induced ovulation is a maternal effect.

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Section: Preimplantation Embryo Development In the Ratcontrasting

confidence: 99%

Section: Embryo Survival At Days 12-14 O F Gestationmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 94%

“…have been described extensively before [5,6]. A 10-hour dark period commenced at 5.00 p.m. Estrus cycles were followed by daily vaginal lavage.…”

Section: Ratsmentioning

confidence: 99%

“…When an analysis of Figures in parentheses are number of females. a Results from Mattheij et al [5]. b Results from Boerjan and de Boer [8], Table 2.…”

Section: Preimplantation Embryo Development In the Ratmentioning

confidence: 99%

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Embryo Loss, Blastomere Development and Chromosome Constitution after Human Chorionic Gonadotropin-Induced Ovulation in Mice and Rats with Regular Cycles

Boer¹,

Hoeven²,

Wolters³

et al. 1991

Gynecol Obstet Invest

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First cell cycle of zygotes of the mouse derived from oocytes aged postovulation in vivo and fertilized in vivo

Boerjan

Boer

1990

Molecular Reproduction Devel

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This paper describes an analysis of the first cell cycle of mouse oocytes aged postovulation and fertilized in vivo. For this purpose, we developed a procedure for inducing ovulation in vivo that allows accurate timing of ovulation. The method is based on a luteinizing hormone (LH)-releasing hormone (LHRH) administration at proestrus. This ovulation procedure had no detectable effect on the rate of ovulation or postimplantation embryonic death. We used this method of ovulation induction in an analysis of the separate stages of the first cell cycle of in vivo fertilized postovulation aged oocytes. All stages assessed were shorter in aged oocytes (12 hr postovulation) than in zygotes from unaged oocytes (1 hr postovulation): 1) the time interval between insemination and penetration of the aged oocytes was 1.5 hr shorter than the time interval of the unaged oocytes; 2) pronuclear formation in the fertilized aged oocytes was somewhat quicker than pronuclear formation in fertilized unaged oocytes; 3) in zygotes from aged oocytes, the time between formation of pronuclei and the pronuclear membrane breakdown was 1 hr shorter than in zygotes from unaged oocytes; 4) the first cleavage division was 3 hr advanced in zygotes from aged oocytes compared with the moment of the first cleavage division in zygotes from unaged oocytes. We also determined the glutathione (GSH) content of unaged and aged oocytes to investigate a possible relationship between the rate of pronuclear formation and GSH. The level of GSH was two times lower in oocytes aged postovulation for 12 hr than in unaged oocytes.2+ level of GSH in fertilized, unaged oocytes was half that in

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Induction of ovulation by a potent, orally active, low molecular weight agonist (Org 43553) of the luteinizing hormone receptor

Lagemaat¹,

Timmers²,

Kelder³

et al. 2008

Human Reproduction

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Administration of Human Chorionic Gonadotropin but Not of Luteinizing Hormone-Releasing Hormone at Pro-Oestrus or Late Di-Oestrus Has a Deleterious Effect on Pregnancy in the Rat

Cited by 8 publications

References 13 publications

Embryo Loss, Blastomere Development and Chromosome Constitution after Human Chorionic Gonadotropin-Induced Ovulation in Mice and Rats with Regular Cycles

Embryo Loss, Blastomere Development and Chromosome Constitution after Human Chorionic Gonadotropin-Induced Ovulation in Mice and Rats with Regular Cycles

First cell cycle of zygotes of the mouse derived from oocytes aged postovulation in vivo and fertilized in vivo

Induction of ovulation by a potent, orally active, low molecular weight agonist (Org 43553) of the luteinizing hormone receptor

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