Administration of isothiocyanates enhances heat tolerance in Arabidopsis thaliana

Hara, Masakazu; Harazaki, Akino; Tabata, Kyoko

doi:10.1007/s10725-012-9748-5

Cited by 53 publications

(46 citation statements)

References 25 publications

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“…This phenomenon indicated that some adverse impacts from endogenous JA deficiency may be alleviated by exogenous MeJA application. In addition to antibacterial activity and insect resistance, isothiocyanates also act as heat tolerance enhancers in plant45. Khokon et al .…”

Section: Resultsmentioning

confidence: 99%

Effects of MeJA on Arabidopsis metabolome under endogenous JA deficiency

Cao

Chen

et al. 2016

Sci Rep

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Jasmonates (JAs) play important roles in plant growth, development and defense. Comprehensive metabolomics profiling of plants under JA treatment provides insights into the interaction and regulation network of plant hormones. Here we applied high resolution mass spectrometry based metabolomics approach on Arabidopsis wild type and JA synthesis deficiency mutant opr3. The effects of exogenous MeJA treatment on the metabolites of opr3 were investigated. More than 10000 ion signals were detected and more than 2000 signals showed significant variation in different genotypes and treatment groups. Multivariate statistic analyses (PCA and PLS-DA) were performed and a differential compound library containing 174 metabolites with high resolution precursor ion-product ions pairs was obtained. Classification and pathway analysis of 109 identified compounds in this library showed that glucosinolates and tryptophan metabolism, amino acids and small peptides metabolism, lipid metabolism, especially fatty acyls metabolism, were impacted by endogenous JA deficiency and exogenous MeJA treatment. These results were further verified by quantitative reverse transcription PCR (RT-qPCR) analysis of 21 related genes involved in the metabolism of glucosinolates, tryptophan and α-linolenic acid pathways. The results would greatly enhance our understanding of the biological functions of JA.

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Section: Resultsmentioning

confidence: 99%

Effects of MeJA on Arabidopsis metabolome under endogenous JA deficiency

Cao

Chen

et al. 2016

Sci Rep

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“…Among the volatile forms methyl salicylate [128] and methyl benzoate have an important activity in plant defense from pathogens [129]. Another well-known benzenoid is salicylic acid (SA), a plant hormone that has been traditionally involved in pathogen defense [130] but has been proved effective in alleviating the damage induced by several abiotic stress conditions [131]. Mechanistically, SA may induce little bursts of H 2 O 2 production resulting in mild oxidative stress which, in turn, could enhance the antioxidant activity, improving stress tolerance [132].…”

Section: Effect Of Abiotic Stress On Plant Biochemistry: Metabolitmentioning

confidence: 99%

Metabolomics as a Tool to Investigate Abiotic Stress Tolerance in Plants

Arbona

Manzi²,

Ollas

2013

IJMS

542

316

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Metabolites reflect the integration of gene expression, protein interaction and other different regulatory processes and are therefore closer to the phenotype than mRNA transcripts or proteins alone. Amongst all –omics technologies, metabolomics is the most transversal and can be applied to different organisms with little or no modifications. It has been successfully applied to the study of molecular phenotypes of plants in response to abiotic stress in order to find particular patterns associated to stress tolerance. These studies have highlighted the essential involvement of primary metabolites: sugars, amino acids and Krebs cycle intermediates as direct markers of photosynthetic dysfunction as well as effectors of osmotic readjustment. On the contrary, secondary metabolites are more specific of genera and species and respond to particular stress conditions as antioxidants, Reactive Oxygen Species (ROS) scavengers, coenzymes, UV and excess radiation screen and also as regulatory molecules. In addition, the induction of secondary metabolites by several abiotic stress conditions could also be an effective mechanism of cross-protection against biotic threats, providing a link between abiotic and biotic stress responses. Moreover, the presence/absence and relative accumulation of certain metabolites along with gene expression data provides accurate markers (mQTL or MWAS) for tolerant crop selection in breeding programs.

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“…LudwigMüller et al (2000) also observed that the TU8 mutant of Arabidopsis, which has a lack in glucosinolate metabolism, was significantly more affected by high temperature than wild-type plants. Moreover, the exogenous application of phenethyl isothiocyanate to A. thaliana plants enhanced heat tolerance, increasing the expression levels of HSP70 (heat shock protein) genes, therefore this isothiocyanate could be involved in the resistance against high temperature stress (Hara et al 2013). …”

Section: Defense Against Abiotic Stressmentioning

confidence: 99%

Biosynthesis and bioactivity of glucosinolates and their production in plant in vitro cultures

Sánchez-Pujante

Borja-Martínez

Pedreño

et al. 2017

Planta

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Main conclusion Glucosinolates are biologically active compounds which are involved in plant defense reaction. The use of plant in vitro cultures and genetic engineering is a promising strategy for their sustainable production.Glucosinolates are a class of secondary metabolites found mainly in Brassicaceae, which contain nitrogen and sulfur in their structures. Glucosinolates are divided into three groups depending on the amino acid from which they are biosynthesized. Aliphatic glucosinolates are generally derived from leucine, valine, methionine, isoleucine and alanine while indole and aromatic glucosinolates are derived from tryptophan and phenylalanine or tyrosine, respectively. These compounds are hydrolyzed by the enzyme myrosinase when plants are stressed by biotic and abiotic factors, obtaining different degradation products. Glucosinolates and their hydrolysis products play an important role in plant defense responses against different types of stresses. In addition, these compounds have beneficial effect on human health because they are strong antioxidants and they have potent cardiovascular, antidiabetic, antimicrobial and antitumoral activities. Due to all the properties described above, the demand for glucosinolates and their hydrolysis products has enormously increased, and therefore, new strategies that allow the production of these compounds to be improved are needed. The use of plant in vitro cultures is emerging as a biotechnological strategy to obtain glucosinolates and their derivatives. This work is focused on the biosynthesis of glucosinolates and the bioactivity of these compounds in plants. In addition, a detailed study on the strategies used to increase the production of several glucosinolates, in particular those synthesized in Brassicaceae, using in vitro plant cultures has been made. Special attention has been paid for increasing the production of glucosinolates and their derivatives using metabolic engineering.

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Administration of isothiocyanates enhances heat tolerance in Arabidopsis thaliana

Cited by 53 publications

References 25 publications

Effects of MeJA on Arabidopsis metabolome under endogenous JA deficiency

Effects of MeJA on Arabidopsis metabolome under endogenous JA deficiency

Metabolomics as a Tool to Investigate Abiotic Stress Tolerance in Plants

Biosynthesis and bioactivity of glucosinolates and their production in plant in vitro cultures

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