Administration of the nonsteroidal anti-inflammatory drug tolfenamic acid at embryo transfer improves maintenance of pregnancy and embryo survival in recipient mice

Abstract

Purpose To evaluate the effect of the nonsteroidal antiinflammatory drugs tolfenamic acid and flunixin meglumine in pregnancy rate and embryo survival of recipient mice subjected to embryo transfer. Methods A total of 142 recipient females were transferred with 2,931 embryos and treated with a single injection of tolfenamic acid (1 mg/kg; n=54 females with 1,129 embryos), flunixin meglumine (2.5 mg/kg; n=46 females with 942 embryos), or bi-distilled water (10 mL/kg) as control group (n=42 females with 860 embryos). Pregnancy was checked 2 weeks after embryo transfer, delivery was registered on the due date, and litter size was recorded on Day 7 after birth. Results Pregnancy rate of tolfenamic acid treated females was significantly higher than flunixin group (P<0.05) and showed a tendency to be higher when compared to the control group (P=0.06). The number of pups born from transferred embryos in pregnant females was significantly higher for both treatment groups compared to controls (P<0.05). Number of pups from total transferred embryos was higher for both treatment groups (P<0.05) when compared to controls. Conclusion The use of tolfenamic acid at the time of embryo transfer improves both pregnancy rate and number of live pups in recipient mice, with optimal effects observed with flunixin meglumine. We suggest that the use of tolfenamic acid has beneficial effects on the maintenance of pregnancy and embryo survival in recipient mice, which should be taken into account for further studies in other mammalian females.

Voluntary intake of paracetamol-enriched drinking water and its influence on the success of embryo transfer in mice

“…In another study, application of tolfenamic acid or flunixin led to a higher pregnancy rate and higher numbers of offspring than in animals undergoing ET without analgesic treatment (Schlapp et al, 2015). A report on multimodal analgesia (recipient female mice received carprofen together with buprenorphine) also showed no significant adverse effects on the results of ET in mice (Parker et al, 2011).…”

Long-Term Effect of Environmental Enrichment on Reproductive Performance of Swiss Webster Mice and Their Female Offspring

“…ET was conducted in a randomly blocked design for each treatment (EE and control group), regarding embryo stage (956 zygotes and 465 morulae) and the operator (two trained technicians). Embryo transfer, surgical care and pain relief with 1 mg/kg Tolfenamic acid was performed as previously described [ 19 , 20 ]. Embryos were loaded into a pulled glass pipette and placed through the infundibulum into both oviducts, delivering in average 25–30 zygotes or 17–20 morulae per female.…”

Familial primary ovarian insufficiency associated with aSYCE1point mutation: Defective meiosis elucidated in humanized mice

“…Protospacer adjacent motif (PAM) was disrupted in the ssODN in order to avoid repeated nuclease action after the desired edition. Mice and zygote manipulation for genome editing was performed as previouly described [46, 47]. For details, see Expanded discussion of the Materials and Methods .…”