Seepage in a medium packed with particles of variable granular size can be seen in many fields of engineering applications. Due to the relative complex spatial aspect of pore geometry, there are notable differences in the critical parameters of flow transition (Reynolds number and Forchheimer number) between different structures. It is difficult to distinguish the available range of seepage equations and predict the water flux accurately. This work aims to establish the relationship between particle size and flow transition. This is conducted according to the results of flow region division, which obtains the application range for seepage equations. Experiments were carried out in sand columns with nine different particle sizes of sand with mean diameters of 0.0375, 0.1125, 0.225, 0.45, 0.8, 1.5, 2.18, 3.555 and 7.125 mm. Four flow regimes were identified (pre-Darcy regime, Darcy regime, Forchheimer regime and turbulent regime). The experimental data indicate that the permeability increases exponentially and the inertia factor reduces exponentially with an increase in particle diameter. The inertial effect becomes more significant in the medium with larger particles than with finer particles when the flow transition occurs.