Adoptive transfer of suppression of the autoimmune response to rat male accessory glands: characterization of the suppressor cells

Ferro, Maria Elena; Yranzo‐Volonté, Nora; Riera, Clelia M.

doi:10.1016/0165-2478(87)90119-2

Cited by 13 publications

(2 citation statements)

References 28 publications

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“…Moreover, the inhibition was antigen specific because the RAG FI-pretreated animals could effectively mount an immune response to Tuxoplasma gondii antigens [20]. The cell types involved in the suppression included MRAG-specific, cyclophosphamide CY-sensitive, inducer-phase Ts cells, CYand irradiation-sensitive, effector-phase suppressor cells and I-E+ APC [7,21]. The results presented here show clearly that it is more difficult to induce suppression to autoantigen of RAG in old rats.…”

Section: Discussionmentioning

confidence: 66%

Effect of aging on the autoimmune response to rat male accessory glands: deficit of I‐E‐positive peritoneal cells capable of inducing suppression

1991

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The present report analyzes the ability to induce suppression to rat male accessory gland (RAG) autoantigens and the characteristics of T suppressor (Ts)-inducer peritoneal cells (PC) in old rats which show increased autoimmune responses. The injection of young rats with a purified fraction (FI) of RAG 10 and 3 days prior to immunization with chemically modified RAG (MRAG) markedly reduced the immune response to RAG autoantigens when compared with young rats which had only been immunized (controls), while the pretreatment of old rats did not block the delayed-type hypersensitivity reaction to MRAG when compared with control old rats. The study of cell surface markers on PC from rats injected i.p. 2 h previously with FI of RAG (FI-PC) showed an increase of OX-6 (I-A) and a decrease of OX-17 (I-E) in FI-PC of old rats with respect to FI-PC of young animals, which showed a selective increase of I-E+ Ts-inducer PC. The i.p. injection of FI-PC from old rats into young recipients, 10 and 3 days prior to immunization with MRAG in complete Freund's adjuvant, did not modify the autoimmune response when compared with controls. By contrast, the injection of young and old rats with FI-PC from young animals induced a significant suppression of the autoimmune response. The reduced percentage of I-E+ suppressor-inducer PC provides an explanation for the diminished ability to induce suppression to RAG autoantigens in old rats.

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Section: Discussionmentioning

confidence: 66%

Effect of aging on the autoimmune response to rat male accessory glands: deficit of I‐E‐positive peritoneal cells capable of inducing suppression

1991

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“…We have been involved in the study of immunoregulatory mechanisms as they relate the expression of experimental autoimmune response to RAG. 1,2,15 Since the soluble RAG is a poor immunogen even though incorporated to CFA, an experimental model of autoimmune response to RAG was developed immunizing rats with MRAG-CFA. t Later on and with the purpose of using the antigen in its native form, we assessed both the primary and the secondary immune responses to liposome-associated RAG.…”

Section: Munksgmrd Copenhagenmentioning

confidence: 99%

Characterization of an Immunoregulatory Cell Subset Involved in the Upregulation of the Autoimmune Response to Rat Male Accessory Glands

Iribarren¹,

Correa²,

Riera³

1993

American J Rep Immunol

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Adult male Wistar rats were submitted to three intraperitoneal (i.p.) immunizations with 750 micrograms of saline extract of RAG associated with liposomes. The delayed type hypersensitivity (DTH) response studied approximately 10 days after each immunization developed after the first immunization, having a remission state after the second one and a clear increase after the third injection. In a further study, spleen mononuclear (SpM) cells obtained form immunized rats 10 days after the third immunization (DTH positive) or from normal rats were separated as adherent (VV+) or nonadherent (VV-) to Vicia villosa population. In VV+ SpM cells from immunized or normal animals an enhanced percentage of OX8+ cells (P < .05 and P < .01, respectively) was found, but in VV- SpM cells from the same groups of rats an enhanced percentage of W3/25+ cells (P < .01 and P < .05, respectively) was found when they were studied by immunofluorescence. Later on, we transferred total VV+ or VV- SpM cells from i.p. immunized rats to immunized recipients 10 days after the second immunization (DTH negative). The DTH response was enhanced in recipients of total or VV+ SpM cells (P < .01). It was also observed that the transfer of VV- SpM cells from immunized rats or total or VV+ SpM cells from normal rats did not reduce the suppression state observed after the second injection (P = NS). The total SpM cells obtained 10 days after the third immunization (DTH positive) were able to transfer autoimmune response to RAG to normal animals (P < .01), whereas VV+ SpM cells did not show that capacity (P = NS).

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Antigen‐induced inhibition of autoimmune response to rat male accessory glands: distinct role of I‐A and I‐E‐positive peritoneal cells

1990

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The present report describes the structural and functional characteristics of the population of peritoneal cells (PC) from rats injected intraperitoneally (i.p.) 2 h or 24 h previously with a suppressor dose of a purified fraction (FI) of rat male accessory glands (RAG: FI-PC2h and FI-PC24h, respectively). Both populations of PC showed the presence of the autoantigens of RAG on their membrane. The study of cell surface marker showed increase of OX17 (I-E) in FI-PC2h and OX6 (I-A) in FI-PC24h. I.p. injection of FI-PC2h 10 and 3 days prior to immunization with chemically modified (diazotized arsanilic and sulfanilic acid) RAG (MRAG) in complete Freund's adjuvant induced suppression of the delayed-type hypersensitivity (DTH) response to MRAG, whereas the i.p. injection of FI-PC24h induced potentiation of DTH response to MRAG when compared with controls (animals injected 10 and 3 days prior to immunization with PC from rats injected i.p. with an unrelated antigen). The treatment of FI-PC2h with OX17 and FI-PC24h with OX6 prior to the transfer blocked the suppression and potentiation described above. These findings show that the i.p. injection of an FI of RAG at a suppressor dose induces PC with immunologically opposite characteristics, depending on the time of obtention.

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Adoptive transfer of suppression of the autoimmune response to rat male accessory glands: characterization of the suppressor cells

Cited by 13 publications

References 28 publications

Effect of aging on the autoimmune response to rat male accessory glands: deficit of I‐E‐positive peritoneal cells capable of inducing suppression

Effect of aging on the autoimmune response to rat male accessory glands: deficit of I‐E‐positive peritoneal cells capable of inducing suppression

Characterization of an Immunoregulatory Cell Subset Involved in the Upregulation of the Autoimmune Response to Rat Male Accessory Glands

Antigen‐induced inhibition of autoimmune response to rat male accessory glands: distinct role of I‐A and I‐E‐positive peritoneal cells

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