ADP/ATP translocator is essential only for anaerobic growth of yeast <i>Saccharomyces cerevisiae</i>

Drgon, Tomás; Sabová, L; Nelson, Nathan; Kolarov, Jordan

doi:10.1016/0014-5793(91)81059-h

Cited by 89 publications

(88 citation statements)

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“…This ®nding is consistent with the absence of cytochrome c translocation in r 0 cells, while dissociation between cytochrome c release and mitochondrial potential change has been reported (Bossy-Wentzel et al, 1998). Previous papers employing mammalian cells or yeast reported that mitochondrial potential is preserved in mitochondrial DNA-depleted cells, owing to compensatory adenine nucleotide translocator function allowing countertransport of adenine nucleotides across the mitochondrial membrane (Buchet and Gedinot, 1998;Drgon et al, 1991;Skowronek et al, 1992). No signi®cant basal and glucose-stimulated mitochondrial proton gradient previously observed in SK-Hep1 r 0 cells might be due to the di erence in the method of measurement between¯ow cytometry and quenching method (Park et al, 2001).…”

Section: Discussionmentioning

confidence: 96%

Resistance of mitochondrial DNA-deficient cells to TRAIL: role of Bax in TRAIL-induced apoptosis

Kim

Chang

et al. 2002

Oncogene

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Section: Discussionmentioning

confidence: 96%

Resistance of mitochondrial DNA-deficient cells to TRAIL: role of Bax in TRAIL-induced apoptosis

Kim

Chang

et al. 2002

Oncogene

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“…Published procedures were used for preparation of total RNA from yeast cells [14], protein extraction from yeast cells [16], immuno-blot analysis of AAC proteins [4], and transformation of yeast cells by lithium acetate treatment [17]. For the Northern blots, total RNA was electrophoresed on a denaturing 1.2% agarose gel containing formaldehyde [14].…”

Section: Methodsmentioning

confidence: 99%

“…myces cerevisiae, it is also essential for growth under anaerobic conditions [4]. Previous studies have indicated that the content of ADP/ATP carrier in the mitochondrial membrane is affected by the growth conditions, and by mitochondrial-respiratory-deficient (e-) mutations [5-71.…”

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confidence: 99%

Expression of the AAC2 Gene Encoding the Major Mitochondrial ADP/ATP Carrier in Saccharomyces cerevisiae is Controlled at the Transcriptional Level by Oxygen, Heme and HAP2 Factor

Betina

Gavurníková

Haviernik

et al. 1995

European Journal of Biochemistry

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Expression of the Saccharomyces cerevisiae AAC2 gene encoding the major mitochondrial ADP/ATP carrier was examined. The intracellular level of the carrier protein, as well as the level of the AAC2-genespecific mRNA, is influenced by the presence or absence of oxygen or of heme, and it is subject to carbon-source control. In addition, the expression of AAC2 gene requires the products of the HAP2 and HAP3 genes, but not that of the HAP1 gene. The 5'-flanking region of the gene was isolated, sequenced and fused to the lacZ reporter gene in order to study the effect of carbon sources and of specific deletion mutations on expression of the gene in yeast transformants. The expression of the reporter gene reveals that the AAC2 gene possesses a strong inducible promoter. The promoter analysis, combined with expression studies in the wild-type as well as in various mutant strains, identified an upstream activation site (UAS) contained within a sequence between -393 bp and -268 bp, and several major initiation sites of AAC2 mRNA between -105 bp and -95 bp. Deletion analysis also shows that the TATA boxes located 45 bp and 104 bp upstream of the 5'-ends of AAC2 mRNA are not essential for the transcription. The UAS of the AAC2 gene is required for activation by HAP2 and heme and for release from glucose repression. A restriction fragment containing the UAS conferred oxygen and carbon source regulation when placed upstream of another yeast gene encoding ADP/ATP carrier (AAC3), deleted of its regulatory sequences. The UAS of the AAC2 gene contains at least two distinct motifs for DNA-binding transcriptional activators, including one which is identical with the core HAP21314 binding motif, and a second one with the ABFI consensus binding sequence. Our results indicate that these sequences mediate the effects of the respective transactivator on the oxygen-and carbon-source-dependent transcription of the AAC2 gene.Keywords. ADP/ATP carrier ; heme ; HAP2 transcription factor ; transcription ; Saccharomyces cerevisiae.The ADP/ATP carrier is a nuclear-encoded integral protein of the inner mitochondrial membrane. The functional protein forms a homodimer of two approximately 30-kDa subunits through which ADP and ATP are exchanged between the mitochondria and the cytoplasm [l]. The ADP/ATP carrier is essential for oxidative phosphorylation, as mutations in the gene encoding this protein in yeast cells result in phenotypes unable to grow on nonfermentable carbon sources [2, 31. The key function of this protein in the link between mitochondrial and cytosolic metabolism is further underlined by the fact that, in SaccharoCorrespondence to J. Kolarov, Department of Biochemistry, Comenius University, Faculty of Sciences, Mlynska dolina CH-1, 842 15 Bratislava, SlovakiaFax: +427 729 064 or +427 326 140. Abbreviations. UAS, upstream activation site ; URS, upstream repressor site; UP, rich growth medium composed by 1% yeast extract and 2% bactopeptone; SM, minimal synthetic growth medium containing 0.67 % yeast nitrogen base and 2% dextrose.En...

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“…While AACI and AAC2 are expressed preferentially )ruder derepressed conditions, the AAC3 gone is expressed only under anaerobic conditions [3]. Among these three genes only AAC2 encodes a translocator protein functioning in the ADP/ATP transport during oxidative phosphorylation [2][3][4][5][6]. On the other hand the AAC1 and AAC3 genes are not essential for growth on respiratory carbon sources and their products cannot be detected in the mitochondria of cells grown under different conditions [4].…”

Section: Introductionmentioning

confidence: 99%

“…Among these three genes only AAC2 encodes a translocator protein functioning in the ADP/ATP transport during oxidative phosphorylation [2][3][4][5][6]. On the other hand the AAC1 and AAC3 genes are not essential for growth on respiratory carbon sources and their products cannot be detected in the mitochondria of cells grown under different conditions [4]. Thus, the cellular function of the products of the AACl and AAC3 genes remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Yeast ADP/ATP carrier (AAC) proteins exhibit similar enzymatic properties but their deletion produces different phenotypes

et al. 1992

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Saccharomyces cerevisiae strains expressing a single type of ADP/ATP carrier (AAC) protein were prepared from a mutant in which all AAC genes were disrupted, by transformatior, with plasmids containing a chosen AAC gone. As demonstrated by measurements of [)~C]ADP six'fie binding and transport, all three translocator proteins, AACI, AAC2 and AAC3 when present in the mitochondrial membrane, exhibited similar transloca:ion properties, The disruption of some AAC genes, however, resulted in phenotypes indicating that the function of these proteins m whole cells can be quite different, Specifically, we found that the disruption of AAC 1 gone, but not AAC2 and AAC3, resulted in a change in colony phenotype.

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ADP/ATP translocator is essential only for anaerobic growth of yeast Saccharomyces cerevisiae

Cited by 89 publications

References 14 publications

Resistance of mitochondrial DNA-deficient cells to TRAIL: role of Bax in TRAIL-induced apoptosis

Resistance of mitochondrial DNA-deficient cells to TRAIL: role of Bax in TRAIL-induced apoptosis

Expression of the AAC2 Gene Encoding the Major Mitochondrial ADP/ATP Carrier in Saccharomyces cerevisiae is Controlled at the Transcriptional Level by Oxygen, Heme and HAP2 Factor

Yeast ADP/ATP carrier (AAC) proteins exhibit similar enzymatic properties but their deletion produces different phenotypes

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