ADP is a vasodilator component from Lasiodora sp. mygalomorph spider venom

Horta, Carolina Campolina Rebello; Rezende, Bruno Almeida; Oliveira-Mendes, Bárbara Bruna Ribeiro; Carmo, Anderson Oliveira do; Capettini, Luciano dos Santos Aggum; Silva, Josiane F.; Gomes, Marco Túlio R.; Chávez-Olórtegui, Carlos; Bravo, Carlos; Lemos, Virgı́nia S.; Kalapothakis, Evanguedes

doi:10.1016/j.toxicon.2013.06.006

Cited by 18 publications

(16 citation statements)

References 64 publications

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“…Aird [ 43 ] found that only a small number of snake venoms contained AMP compared with adenosine, but stated that the presence of purine monophosphates within venoms is unsurprising due to the hypotensive effects they share with free purines. We are unaware of previous reports of AMP, adenosine diphosphate (ADP), or adenosine triphosphate (ATP) from scorpion venoms; however, they are present within different tarantula venoms, including those belonging to H. lividum , Lasiodora sp., Aphonopelma sp., and Aphonopelma hentzi ( Dugesiella hentzi and Eurypelma californicum ) [ 30 , 33 , 52 , 66 , 67 ]. Unlike adenosine, which binds to P1 receptors, ADP and ATP bind to P2 receptors [ 68 ], with evidence suggesting that they also have auxiliary roles alongside toxins within venom [ 30 , 66 ].…”

Section: Discussionmentioning

confidence: 99%

“…We are unaware of previous reports of AMP, adenosine diphosphate (ADP), or adenosine triphosphate (ATP) from scorpion venoms; however, they are present within different tarantula venoms, including those belonging to H. lividum , Lasiodora sp., Aphonopelma sp., and Aphonopelma hentzi ( Dugesiella hentzi and Eurypelma californicum ) [ 30 , 33 , 52 , 66 , 67 ]. Unlike adenosine, which binds to P1 receptors, ADP and ATP bind to P2 receptors [ 68 ], with evidence suggesting that they also have auxiliary roles alongside toxins within venom [ 30 , 66 ]. As with adenosine, ADP and AMP possess hypotensive properties and may fulfil a similar auxiliary function within the venom [ 8 , 66 ].…”

Section: Discussionmentioning

confidence: 99%

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Small Molecules in the Venom of the Scorpion Hormurus waigiensis

et al. 2020

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Despite scorpion stings posing a significant public health issue in particular regions of the world, certain aspects of scorpion venom chemistry remain poorly described. Although there has been extensive research into the identity and activity of scorpion venom peptides, non-peptide small molecules present in the venom have received comparatively little attention. Small molecules can have important functions within venoms; for example, in some spider species the main toxic components of the venom are acylpolyamines. Other molecules can have auxiliary effects that facilitate envenomation, such as purines with hypotensive properties utilised by snakes. In this study, we investigated some non-peptide small molecule constituents of Hormurus waigiensis venom using LC/MS, reversed-phase HPLC, and NMR spectroscopy. We identified adenosine, adenosine monophosphate (AMP), and citric acid within the venom, with low quantities of the amino acids glutamic acid and aspartic acid also being present. Purine nucleosides such as adenosine play important auxiliary functions in snake venoms when injected alongside other venom toxins, and they may have a similar role within H. waigiensis venom. Further research on these and other small molecules in scorpion venoms may elucidate their roles in prey capture and predator defence, and gaining a greater understanding of how scorpion venom components act in combination could allow for the development of improved first aid.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Small Molecules in the Venom of the Scorpion Hormurus waigiensis

et al. 2020

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“…Endothelium-derived nitric oxide has also been implicated in the vasodilation caused by Lasiodora sp. venom (Horta et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…For example, adenosine 59-diphosphate is the principal mediator of edema caused by venom of the theraphosid Lasiodora sp. (Horta et al, 2013), whereas adenosine 59-diphosphate and ATP may actually inhibit edema caused by P. nigriventer venom (Costa et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Pharmacological Characterization of the Edema Caused by Vitalius dubius (Theraphosidae, Mygalomorphae) Spider Venom in Rats

Silva

Linardi

Antunes

et al. 2016

The Journal of Pharmacology and Experimental Therapeutics

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Bites by tarantulas (Theraphosidae, Mygalomorphae) in humans can result in mild clinical manifestations such as local pain, erythema, and edema. Vitalius dubius is a medium-sized, nonaggressive theraphosid found in southeastern Brazil. In this work, we investigated the mediators involved in the plasma extravasation caused by V. dubius venom in rats. The venom caused dose-dependent (0.1-100 mg/site) edema in rat dorsal skin. This edema was significantly inhibited by butyl]benzamide) (SR48968, a neurokinin NK 2 receptor antagonist) had no effect on the venom-induced increase in vascular permeability. In rat hind paws, the venom-induced edema was attenuated by ketoprofen (a nonselective COX inhibitor) administered 15 minutes postvenom. Preincubation of venom with commercial antiarachnid antivenom attenuated the venom-induced edema. These results suggest that the enhanced vascular permeability evoked by V. dubius venom involves serotonin, COX products, neurokinin NK 1 receptors, and nitric oxide formation. The attenuation of hind paw edema by ketoprofen suggests that COX inhibitors could be useful in treating the local inflammatory response to bites by these spiders.

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“…Consequently, these results suggest the participation of ERα in the endothelium-and NO-dependent relaxant effect of DGT in the rat aorta. Finally, the participation of bradykinin and P2Y receptors was tested, but neither HOE-140 (1 µM) nor suramin (100 µM), respective antagonists of bradykinin and P2Y receptors [44], induced any significant effect on the concentration-response curve to DGT (Fig. 1 S, Supporting Information).…”

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confidence: 99%

Dihydrogoniothalamin, an Endothelium and NO-Dependent Vasodilator Drug Isolated from Aniba panurensis

et al. 2015

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Dihydrogoniothalamin is a styrylpyrone isolated from the leaves of Aniba panurensis. The present work aimed at investigating the vasorelaxant activity of dihydrogoniothalamin and its underlying mechanism of action in the rat aorta. Dihydrogoniothalamin (0.01-100 µM) induced a concentration-dependent vasodilatation of aortas precontracted with phenylephrine. Endothelium removal or pretreatment of the preparation with NG nitro-L-arginine-methyl-ester abolished the vasodilator response for dihydrogoniothalamin. Pretreatment with calmidazolium did not affect the vasodilator response of dihydrogoniothalamin. On the other hand, wortmannin, a nonselective inhibitor of phosphatidylinositol 3-kinases, and protein kinase B inhibitor IV significantly shifted the concentration-response curve of dihydrogoniothalamin to the right and reduced its maximal effect. A nonselective antagonist of estrogen receptors, ICI 182,780, and a selective antagonist of estrogen receptor α, methyl-piperidino-pyrazole, were able to reduce the relaxation induced by dihydrogoniothalamin, but no effect was observed in the presence of the selective antagonists of estrogen receptor β and G protein-coupled receptor 30, 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol (PHTPP), and G-15, respectively. Dihydrogoniothalamin also increased the phosphorylation of the activation sites of endothelial nitric oxide synthase and protein kinase B. The present results led us to conclude that dihydrogoniothalamin is a vasodilator drug acting in an endothelium- and nitric oxide-dependent manner through a mechanism involving the activation of nitric oxide synthase via the phosphatidylinositol 3-kinase/protein kinase B pathway, partially by stimulation of estrogen receptor α.

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ADP is a vasodilator component from Lasiodora sp. mygalomorph spider venom

Cited by 18 publications

References 64 publications

Small Molecules in the Venom of the Scorpion Hormurus waigiensis

Small Molecules in the Venom of the Scorpion Hormurus waigiensis

Pharmacological Characterization of the Edema Caused by Vitalius dubius (Theraphosidae, Mygalomorphae) Spider Venom in Rats

Dihydrogoniothalamin, an Endothelium and NO-Dependent Vasodilator Drug Isolated from Aniba panurensis

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