ADP-ribosylation factors improve biomass yield and salinity tolerance in transgenic switchgrass (Panicum virgatum L.)

Guan, Cong; Li, Xue; Tian, Danyang; Liu, Hua-Yue; Cen, Huifang; Tadege, Million; Zhang, Yunwei

doi:10.1007/s00299-020-02589-x

Cited by 10 publications

(7 citation statements)

References 56 publications

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“…Additionally, ectopically expressing maize ZmARF2 in Arabidopsis increased leaf and seed size, thus augmenting overall plant growth [ 30 ]. Overexpressing PvARF1 , PvARF-B1C , and PvARF-related in switchgrass enhanced biomass by improving tillers, height, and leaf length [ 19 ]. ARF family proteins are classified into two subclades: ARFs and ARLs.…”

Section: Discussionmentioning

confidence: 99%

“…The ectopic expression of Medicago falcate MfARL1 in Arabidopsis enhanced the salt tolerance of the transgenic plant [ 18 ]. Previous research suggested that the overexpression of PvARFs ( PvArf1 -, PvArf-B1C -, and PvArf-related ) in switchgrass resulted in improved plant growth and enhanced salt tolerance [ 19 ]. However, there has been no research on the response of ARL genes to alkali stress in switchgrass so far.…”

Section: Introductionmentioning

confidence: 99%

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PvARL1 Increases Biomass Yield and Enhances Alkaline Tolerance in Switchgrass (Panicum virgatum L.)

Li,

Guan,

Liu

et al. 2024

Plants

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Switchgrass is an important bioenergy crop valued for its biomass yield and abiotic tolerance. Alkali stress is a major abiotic stress that significantly impedes plant growth and yield due to high salinity and pH; however, the response mechanism of switchgrass to alkali stress remains limited. Here, we characterized PvARL1, an ARF-like gene, which was up-regulated in both the shoot and root tissues under alkali stress conditions. Overexpression of PvARL1 not only improved alkali tolerance but also promoted biomass yield with more tiller and higher plant height in switchgrass. Moreover, PvARL1 overexpression lines displayed higher capacities in the maintenance of water content and photosynthetic stability compared with the controls under alkali treatments. A significant reduction in the ratio of electrolyte leakage, MDA content, and reactive oxygen species (ROS) showed that PvARL1 plays a positive role in protecting cell membrane integrity. In addition, PvARL1 also negatively affected the K+ efflux or uptake in roots to alleviate ion toxicity under alkali treatments. Overall, our results suggest that PvARL1 functions as a positive regulator in plant growth as well as in the plant response to alkali stress, which could be used to improve switchgrass biomass yield and alkali tolerance genetically.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

PvARL1 Increases Biomass Yield and Enhances Alkaline Tolerance in Switchgrass (Panicum virgatum L.)

Li,

Guan,

Liu

et al. 2024

Plants

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“…ADP ribosylation factor (ARF) family of small GTP binding proteins regulates a wide range of cellular processes in eukaryotes [46]. According to Guan et al , three ARF genes (PvArf1, PvArf-B1C and PvArf-related) were identified and localized in the nucleus and cytoplasm, which regulated proline biosynthesis by physically interacting with PvP5CS1 to improve salt tolerance in Switchgrass (Panicum virgatum L.) [47]. The latest research showed that wheat contains 74 TaARF genes.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide survey of the F-box/Kelch (FBK) members and molecular identification of a novel FBK geneTaAFRin wheat

Wei

Zhao

Fan

et al. 2021

Preprint

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F-box proteins play critical roles in plant responses to biotic/abiotic stresses. In the present study, a total of 68 wheat F-box/Kelch ( TaFBK ) gene sequences encoding for 74 proteins were obtained in a genome-wide survey against EnsemblPlants. The 74 TaFBK proteins were divided into 5 categories based on their domain structures. The FBK proteins from wheat, Arabidopsis, and three other cereal species were grouped into 7 clades, and the number of Kelch domains present was their key clustering criterion. Sixty-eight TaFBK genes were unevenly distributed on 21 chromosomes. Most of TaFBKs were predicted to localize in the nucleus and cytoplasm. In silico analysis of a digital PCR revealed that TaFBKs were expressed at multiple developmental stages and tissues, and in response to drought and/or heat stresses. The TaFBK19 gene, a homologous to the Attenuated Far-Red Response ( AFR ) genes in other plant species, and hence named TaAFR , was selected for further analysis. The gene was isolated from the wheat line TcLr15 and its expression evaluated by quantitative real-time PCR. TaAFR transcripts were primarily detected in wheat leaves, and its expression was found to be regulated by various abiotic and biotic stresses as well as plant signaling hormones. Of particular interest, TaAFR expression was differentially regulated in the compatible vs incompatible wheat leaf rust reaction. Subcellular localization studies showed that TaAFR accumulates in the nucleus and cytoplasm. Three TaAFR-interacting proteins were identified experimentally: Skp1/ASK1-like protein (Skp1), ADP-ribosylation factor 2-like isoform X1 (ARL2) and phenylalanine ammonia-lyase (PAL). Further analysis revealed that the Skp1 protein interacted specifically with the F-box domain of TaAFR, while ARL2 and PAL were recognized by the Kelch domain. The data presented herein provides a solid foundation from which the function and metabolic network of TaAFR and other wheat FBKs can be further explored.

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“…Most of what is known about ARL2 proteins comes from research in humans and yeast, with little information in plants. Some plant ARF genes have been linked with biotic or abiotic stress responses, either by gene expression analysis [50] or through experimental validation [51]. Guan et al characterized three ARF genes from Switchgrass (Panicum virgatum L.), namely PvArf1, PvArf-B1C and PvArf-related [51].…”

Section: Plos Onementioning

confidence: 99%

“…Some plant ARF genes have been linked with biotic or abiotic stress responses, either by gene expression analysis [50] or through experimental validation [51]. Guan et al characterized three ARF genes from Switchgrass (Panicum virgatum L.), namely PvArf1, PvArf-B1C and PvArf-related [51]. Through transgenic overexpression of these genes, the authors determined that they contribute to salt-tolerance in Switchgrass and that then phenotype was linked to an increase in proline accumulation.…”

Section: Plos Onementioning

confidence: 99%

Genome-wide survey of the F-box/Kelch (FBK) members and molecular identification of a novel FBK gene TaAFR in wheat

et al. 2021

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F-box proteins play critical roles in plant responses to biotic/abiotic stresses. In the present study, a total of 68 wheat F-box/Kelch (TaFBK) genes, unevenly distributed across 21 chromosomes and encoding 74 proteins, were identified in EnsemblPlants. Protein sequences were compared with those of Arabidopsis and three cereal species by phylogenetic and domain analyses, where the wheat sequences were resolved into 6 clades. In silico analysis of a digital PCR dataset revealed that TaFBKs were expressed at multiple developmental stages and tissues, and in response to drought and/or heat stresses. The TaFBK19 gene, a homolog of the Attenuated Far-Red Response (AFR) genes in other plant species, and hence named TaAFR, was selected for further analysis. Reverse-transcription quantitative real-time PCR (RT-qPCR) was carried out to determine tissue-specific, hormone and stress (abiotic/biotic) responsive expression patterns. Of interest, TaAFR was expressed most abundantly in the leaves, and its expression in response to leaf rust variants suggests a potential role in compatible vs incompatible rust responses. The protein was predicted to localize in cytosol, but it was shown experimentally to localize in both the cytosol and the nucleus of tobacco. A series of protein interaction studies, starting with a yeast-2-hybrid (Y2H) library screen (wheat leaf infected with incompatible leaf rust pathogens), led to the identification of three TaAFR interacting proteins. Skp1/ASK1-like protein (Skp1) was found to interact with the F-box domain of TaAFR, while ADP-ribosylation factor 2-like isoform X1 (ARL2) and phenylalanine ammonia-lyase (PAL) were shown to interact with its Kelch domain. The data presented herein provides a solid foundation from which the function and metabolic network of TaAFR and other wheat FBKs can be further explored.

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ADP-ribosylation factors improve biomass yield and salinity tolerance in transgenic switchgrass (Panicum virgatum L.)

Cited by 10 publications

References 56 publications

PvARL1 Increases Biomass Yield and Enhances Alkaline Tolerance in Switchgrass (Panicum virgatum L.)

PvARL1 Increases Biomass Yield and Enhances Alkaline Tolerance in Switchgrass (Panicum virgatum L.)

Genome-wide survey of the F-box/Kelch (FBK) members and molecular identification of a novel FBK geneTaAFRin wheat

Genome-wide survey of the F-box/Kelch (FBK) members and molecular identification of a novel FBK gene TaAFR in wheat

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