1985
DOI: 10.1016/s0021-9258(17)38746-x
|View full text |Cite
|
Sign up to set email alerts
|

ADP-ribosylation of transducin by pertussis toxin.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

2
11
0

Year Published

1988
1988
2021
2021

Publication Types

Select...
6
2
1

Relationship

0
9

Authors

Journals

citations
Cited by 95 publications
(13 citation statements)
references
References 41 publications
2
11
0
Order By: Relevance
“…To examine these options, it would be useful to know the binding site on ax for the Byt subunit. The amino-terminal region of ax has been suggested as the binding domain for Byt (Fung & Nash, 1983;Watkins et al, 1985). Navon and Fung (1987) showed by immunoprecipitation, using an anti-at monoclonal antibody, that removal of the amino-terminal region of a, by Staphylococcus aureus V8 protease impairs Byx binding, and the presence of Byt slows down proteolysis.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…To examine these options, it would be useful to know the binding site on ax for the Byt subunit. The amino-terminal region of ax has been suggested as the binding domain for Byt (Fung & Nash, 1983;Watkins et al, 1985). Navon and Fung (1987) showed by immunoprecipitation, using an anti-at monoclonal antibody, that removal of the amino-terminal region of a, by Staphylococcus aureus V8 protease impairs Byx binding, and the presence of Byt slows down proteolysis.…”
Section: Discussionmentioning
confidence: 99%
“…The mAb 4A inhibition of G, ADP-ribosylation was confirmed (lane 2 of Figure 6). Pertussis toxin can also ADP-ribosylate purified a, subunits, although not as well, since the G, complex is the preferred substrate for pertussis toxin (Watkins et al, 1985).…”
mentioning
confidence: 99%
“…The resistance to trypsin digestion in the presence of GTP3,S is a criterion indicating an effective GTP3,S binding to the protein and visualizes the conformational change between the GDP form and the activated form of the c~ subunit (7,9,18,27,28). Consequently, we could assume that (A2-29)Gsa binds GTP3,S and could be used to study the membrane interaction of the mutated protein activated by GTP3,S.…”
Section: Proteolysis Experiments On the Mutant (A2-29)gsc~mentioning
confidence: 99%
“…ADP ribosylation assays that assess the addition of an ADP ribose to α i/t/o in cell membranes have been widely used in studies into the cellular effects of PTx [ 6 , 7 , 151 ]. Early assays monitored ADP ribosylation by PTx using radio-labelled 32 P-NAD + .…”
Section: Cellular Assaysmentioning
confidence: 99%