ADRB3 polymorphism rs4994 (Trp64Arg) associates significantly with bodyweight elevation and dyslipidaemias in Saudis but not rs1801253 (Arg389Gly) polymorphism in ARDB1

Abstract

BackgroundIn some populations, obesity and body weight related disorders show a correlation with polymorphisms in three subtypes of beta-adrenoceptor (β1, β2, and β3) [ADRB1, ADRB2 and ADRB3] genes. We scanned for the polymorphism of Arg389Gly (rs1801253) in ADRB1 and Trp64Arg (rs4994) in ADRB3 genes in Saudi population to determine association, if any, of these polymorphisms with obesity and related disorders.MethodsWe studied 329 non-related adults (33.1% men and 66.9% women), aged 18–36 years. Anthropometric measurements were recorded, and Body mass index (BMI) and waist/hip ratio were calculated; leptin, insulin, lipidogram, and glucose concentrations were determined. ADRB1 and ADRB3 polymorphisms (Arg389Gly and Trp64Arg, respectively) were screened by DNA sequencing. The subjects were divided into three groups according to BMI: normal weight (BMI < 25 kg/m2), overweight (BMI ≥25.1–29.9 kg/m2) subjects, and obese (≥30 kg/m2).ResultsIn the age-matched groups of the normal weight, overweight and obese male and female subjects, all anthropometric parameters were found to be significantly higher, and in the obese group, all biochemical parameters were significantly elevated compared to the normal weight controls. The allelic frequency of Gly389 ADRB1 did not differ amongst the three groups, whereas the frequency of Arg64 of ADRB3 gene was significantly higher in the overweight and obese subjects, compared with the normal weight subjects. In addition, subjects carrying Arg64 allele regardless of their BMI had a greater waist and hip circumference, W/H ratio, plasma cholesterol, triglyceride, LDL, leptin, insulin, and glucose level compared to those with the wild-type Trp allele.ConclusionThe results of this study have shown a significant association between the Trp64Arg polymorphism in ADRB3 gene and the development of overweight and obesity in Saudi populations. It also has an influence on the levels of lipid, insulin, leptin, and glucose, whereas, Arg389Gly polymorphism in ADRB1 is not associated with overweight, obesity or dyslipidaemias in Saudis.

Co-occurrence of an HSPG2 Missense Variant and Functional Polymorphisms in Atypical Schwartz–Jampel Syndrome Type 1 with Obesity: A Case Report

“…7 ADRB3 polymorphisms (Trp64Arg) have been reported to contribute to the development of overweight and obesity. 8 This molecular result most likely explained the overall clinical picture of our patient.…”

“…These genes were previously described as linked to obesity. 7,8 This study was approved by the ethics committee of Santa Maria Nuova Hospital of Reggio Emilia (n. 981) and written informed consent was obtained from the patient's mother.…”

Genes and Eating Preferences, Their Roles in Personalized Nutrition

“…The ADRB3 gene encodes the protein involved in the lipolysis regulation. Rs4994 polymorphism (Figure 8) reduces the sensitivity of protein, changing its structure, due to which there is a slowdown of oxidation and increased fat accumulation, causing obesity [25,26]. Apolipoprotein A5 is the protein that is encoded by the APOA5 gene.…”

Genotyping Method and Frequency of ADRB3-rs4994 Single Nucleotide Polymorphism Genotypes in Hanoi 3-5 Years Old Chidren

“…Sự thay thế này làm ảnh hưởng đến liên kết giữa thụ thể với noradrenalin và protein G trong các tế bào mỡ [4]. Do đó, đa hình này sẽ làm giảm phân giải lipid trong mô mỡ trắng [5], từ đó có thể làm tăng nguy cơ mắc các bệnh chuyển hoá như: đái tháo đường [6], béo phì [7], hội chứng chuyển hoá [8].…”