Adrenal Adenoma with Excess Secretion of Corticosterone and 18-Hydroxycorticosterone

ions of seven steroids were clearly observed in chromatograms of biological samples. The ratio of glucocorticoid (cortisol) to mineralocorticoid (corticosterone) content was measured, and found to be significantly lower for aldosteronomas and higher for Cushing's adenomas than for normal adrenals. These findings indicate that the method had sufficient sensitivity for the identification and simultaneous measurement of a variety of adrenal gland steroids.

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Determination of various steroids in normal adrenal glands and adrenocortical tumours by liquid chromatography/atmospheric pressure ionization mass spectrometry

Komatsu

Morioka

Fujita

et al. 1995

J. Mass Spectrom.

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Adrenocortical Adenoma Producing 18‐Hydroxycorticosterone

et al. 1996

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A 30-year-old man presented at our hospital with microscopic hematuria. Ultrasonography and computed tomography scanning revealed a right adrenal mass measuring 20 x 20mm. The tumor was asymptomatic, but there was obvious accumulation on the right side when scintigraphy was performed with radioactive iodine (1311)-labeled adosterol. Endocrinology studies showed elevation of the plasma cortisol and renin concentrations, while the plasma aldosterone level was low. Right laparoscopic adrenalectomy was done on July 4, 1994. Histologic examination showed an adrenocortical adenoma. Serum levels of adrenocortical hormones were measured before and after surgery, and the tissue content for the same hormones was determined in the resected tumor. The hormonal studies showed that the tumor produc ed 18-hydroxycorticosterone.

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Analysis of Corticoids in Adrenal Glands by High Performance Liquid Chromatography (HPLC)

Morioka

Ohashi²,

Sen³

et al. 1993

Folia Endocrinol

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The HPLC system was used to separate and measure 10 kinds of corticoids in adrenal tissues. Calibration curves were drawn as straight lines that ranged from 1.25 to 20ng, or 1.25 to 200ng by peak area calculated with the chromatointegrator. The samples for the assay were extracted from homogenized tissues and treated with methanol to remove non-steroidal contaminants which may interfere with the ultraviolet absorption monitor. The recovery rate during the assay procedure was calculated using testosterone as the internal standard, because testosterone was not detected in any adrenal tissue examined in the present study. Contents of corticoids were measured in normal adrenal glands obtained during radical nephrectomy for renal cancer and in functioning adrenal adenomas. Steroid levels in the adrenal glands and tumors have been measured by radioimmunoassay until now, and the data obtained in the present study were compared with those in previous reports. Main steroids in normal adrenals were cortisol (F) and corticosterone (B), and there were certain amounts of 11-deoxycortisol (S), 11-deoxycorticosterone (DOC) and precursor steroids. 11 beta-hydroxy-androstenedione was the main androgen in the adrenal gland. Mineralocorticoids other than B and DOC were very low in the normal adrenals. There was a certain balance between the production of cortisol and corticosterone in normal adrenals. In functioning adenomas, the levels of F, B and aldosterone, and F to B ratios (F/B) varied according to their biological features. Although with the HPLC system it was possible to obtain the production balance of each steroid clearly in the chromatogram, we could not detect the delta 5-3 hydroxysteroids such as pregnenolone and dehydroepiandrosterone using the ultraviolet absorption monitor.

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Adrenal Adenoma with Excess Secretion of Corticosterone and 18-Hydroxycorticosterone

Cited by 3 publications

References 9 publications

Determination of various steroids in normal adrenal glands and adrenocortical tumours by liquid chromatography/atmospheric pressure ionization mass spectrometry

Determination of various steroids in normal adrenal glands and adrenocortical tumours by liquid chromatography/atmospheric pressure ionization mass spectrometry

Adrenocortical Adenoma Producing 18‐Hydroxycorticosterone

Analysis of Corticoids in Adrenal Glands by High Performance Liquid Chromatography (HPLC)

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