Adrenomedullin attenuates interleukin-1β-induced inflammation and apoptosis in rat Leydig cells via inhibition of NF-κB signaling pathway

Hu, Wei; Zhou, Panghu; Rao, Ting; Zhang, Xiaobin; Wang, Wei; Zhang, Lijun

doi:10.1016/j.yexcr.2015.10.024

Cited by 39 publications

(38 citation statements)

References 43 publications

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“…Another explanation to the improvement with BM-MSCs was their paracrine effect. This was confirmed by Hu et al, (2017) In this group, there were many large SCN appeared between the follicles when compared to the hypothyroidism induced group which was nearly absent. This might be due to its ability to rearrange as new follicles.…”

Section: Discussionsupporting

confidence: 60%

“…[28] who reported that BM-MSCs paracrine activity had an anti-inflammatory effect and an antiapoptotic effect on intervertebral disc degeneration and this was mediated by nuclear factor-KB and mitochondrial apoptotic pathways in annulus fibrosus cells. Zuo et al, (2019) [29] added that a new specific secretory vesicles which involved in the paracrine effects of BM-MSCs are called exosomes It plays important role in repairing, improved safety and reduced immune rejection.…”

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confidence: 99%

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Role of Stem Cells in Management of Experimentally Induced Hypothyroidism in Albino Rat

Abd-El-Aty¹,

Arafa

2019

The Medical Journal of Cairo University

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Background: Hypothyroidism is a common condition with potentially devastating health consequences that spread worldwide. Stems cells (SCs) based therapy had showed promising benefits in treatment of many diseases on animal models and applied on some human clinical issues. Aim of Study: To investigate the effect of bone marrow mesenchymal stem cells (BM-MSCs) on the biochemical and histological structures of the thyroid gland after experimentally induced hypothyroidism in adult male albino rats. Material and Methods: Forty five adult male Sprague-Dawley albino rats were divided into 3 equal groups: Control group (Group I), Hypothyroidism induced group (Group II); received orally carbimazole 45mg/kg of rat/day for 5 days/week for 4 weeks and Induced hypothyroidism treated with BM-MSCs group (Group III); received oral dose of carbimazole 45mg/kg of rat/day for 5 days/week for 4weeks then received single intravenous injection of 1x10 6 cells of BM-MSCs. This group was subdivided into three equal subgroups according to the time of scarification; Subgroup III6: Scarified at the end of 6th week, Subgroup III8: Scarified at the end of 8 th week and Subgroup III10: Scarified at the end of 10 th week. At the end of the experiment, the hormones of thyroid function were measured and histological examination of the thyroid tissue were done. In addition to morphometric study and statistical analysis. Results: BM-MSCs injection to the induced hypothyroidism rats showed that after 6 and 8 weeks, there were highly significant increase (p<0.01) of freeT3 and T4 values and highly significance decrease of TSH compared to the induced hypothyroidism group. On the other hand, after 10 weeks from injection of BM-MSCs, there were insignificant differences (p>0.05) of free T3, T4 and TSH compared to the induced hypothyroidism group. The histological examination of the induced hypothyroidism after 6 weeks of BM-MSCs injection showed variable degree of response. However, after 8 weeks of BM-MSCs injection, there was universal improve

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Section: Discussionsupporting

confidence: 60%

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confidence: 99%

Role of Stem Cells in Management of Experimentally Induced Hypothyroidism in Albino Rat

Abd-El-Aty¹,

Arafa

2019

The Medical Journal of Cairo University

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“…Previous studies have reported that the therapeutic effects of BMSCs were a result of their differentiation potential; however, reports have increasingly suggested that the functions of BMSCs might be attributable to potent paracrine activities such as cytoprotective factors and exosomes (15,32,35,36). A series of cytoprotective factors secreted by BMSCs have been demonstrated to play the important mediatory function of BMSCs (37)(38)(39)(40). However, according to the established paracrine-mediated mechanism, BMexos are considered to represent a new type of intercellular communication and have been further described as a medium composed of proteins, lipids, mRNAs, and miRNAs.…”

Section: Discussionmentioning

confidence: 99%

BMSCs protect against renal ischemia‐reperfusion injury by secreting exosomes loaded with miR‐199a‐5p that target BIP to inhibit endoplasmic reticulum stress at the very early reperfusion stages

Wang

Zhu

et al. 2019

FASEB j.

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Bone marrow–derived mesenchymal stem cells (BMSCs) have been recently reported to play a variety of vital roles in organ and tissue damage repair, mainly via potent paracrine activity, including secreting extracellular vesicles, such as exosomes, that serve as mediators facilitating intercellular communication and reprogramming recipient cells by delivering their contents to target cells. However, the underlying mechanisms are diverse and complex, and the influencing characteristics have rarely been studied. Accordingly, we designed this study to explore the time dependence of the effects of exosomes derived from BMSCs (BMexos) on renal ischemia‐reperfusion (I/R) injury and the underlying mechanisms associated with the reperfusion time. Impressively, our study is the first to find that BMexos protected against renal I/R injury in vitro and in vivo at the very early reperfusion stages, especially 4–8 h after reperfusion in vitro and 8–16 h after reperfusion in vivo. Interestingly, we simultaneously found that endoplasmic reticulum (ER) stress was significantly suppressed following the administration of BMexos in vitro and in vivo with a similar time dependence. Additionally, we discovered that miR‐199a‐5p, which was abundant in the BMSCs, was transferred into renal tubular epithelial cells (NRK‐52E) in a time‐dependent manner and significantly inhibited I/R‐induced ER stress by targeting binding immunoglobulin protein (BIP). Cocultivation with miR‐199a‐5p‐overexpressing BMSCs amplified the suppression of ER stress and further protected against I/R injury. However, coculture with miR‐199a‐5p‐knockdown BMSCs obviously increased ER stress and reversed the BMexos‐induced protection, and silencing BIP by small interfering RNA–1098 in NRK‐52E inhibited these effects. This study provides evidence that administering BMexos at the very early reperfusion stages significantly protects against renal I/R injury, and ER stress is closely linked to this protection. These results suggest a novel therapeutic strategy during the very early reperfusion stages of renal I/R injury.—Wang, C., Zhu, G., He, W., Yin, H., Lin, F., Gou, X., Li, X. BMSCs protect against renal ischemia‐reperfusion injury by secreting exosomes loaded with miR‐199a‐5p that target BIP to inhibit endoplasmic reticulum stress at the very early reperfusion stages. FASEB J. 33, 5440–5456 (2019). http://www.fasebj.org

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“…Meanwhile, when in vitro-cultured stem cells are injected into the body, the stemness of the MSCs is also reduced [31,32]. Due to the reduction in stemness, MSCs reduce tissue damage mainly through anti-inflammatory effects rather than by differentiating into specific tissue types in tissue damage treatments following myocardial infarction and spinal cord injury [33,34]. The maintenance of MSC stemness is related to multiple factors, including aging, in vitro generation, and origination.…”

Section: Discussionmentioning

confidence: 99%

Melatonin Reverses the Loss of Stemness Induced by TNF-α in Human Bone Marrow Mesenchymal Stem Cells through Upregulation of YAP Expression

Wang

Liang

Qiu

et al. 2019

Stem Cells International

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Mesenchymal stem cells (MSCs) are promising candidates for tissue regeneration and disease treatment. However, long-term in vitro culture results in loss of MSC stemness. The inflammation that occurs at stem cell transplant sites (such as that resulting from TNF-α) is a contributing factor for stem cell treatment failure. Currently, there is little evidence regarding the protective role of melatonin with regard to the negative effects of TNF-α on the stemness of MSCs. In this study, we report a melatonin-based method to reduce the inflammatory effects on the stemness of bone marrow mesenchymal stem cells (BMMSCs). The results of colony formation assays, Alizarin red staining, western blotting, and reverse transcription-polymerase chain reactions suggest that melatonin can reverse the inflammatory damage caused by TNF-α treatment in the third, seventh, and tenth generations of primary BMMSCs (vs. control and the TNF-α-treated group). Meanwhile, a detailed analysis of the molecular mechanisms showed that the melatonin receptor and YAP signaling pathway are closely related to the role that melatonin plays in negative inflammatory effects against BMMSCs. In addition, in vivo experiments showed that melatonin could reverse the damage caused by TNF-α on bone regeneration by BMMSCs in nude mice. Overall, our results suggest that melatonin can reverse the loss of stemness caused by inflammatory factor TNF-α in BMMSCs. Our results also provide a practical strategy for the application of BMMSCs in tissue engineering and cell therapy.

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Adrenomedullin attenuates interleukin-1β-induced inflammation and apoptosis in rat Leydig cells via inhibition of NF-κB signaling pathway

Cited by 39 publications

References 43 publications

Role of Stem Cells in Management of Experimentally Induced Hypothyroidism in Albino Rat

Role of Stem Cells in Management of Experimentally Induced Hypothyroidism in Albino Rat

BMSCs protect against renal ischemia‐reperfusion injury by secreting exosomes loaded with miR‐199a‐5p that target BIP to inhibit endoplasmic reticulum stress at the very early reperfusion stages

Melatonin Reverses the Loss of Stemness Induced by TNF-α in Human Bone Marrow Mesenchymal Stem Cells through Upregulation of YAP Expression

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