ADSCs in a fibrin matrix enhance nerve regeneration after epineural suturing in a rat model

Reichenberger, M; Mueller, Wolf; Hartmann, J.; Diehm, Yannick; Laß, Ulrike; Koellensperger, Eva; Leimer, Uwe; Germann, Günter; Fischer, Sebastian

doi:10.1002/micr.30018

Cited by 40 publications

(28 citation statements)

References 49 publications

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“…Although this period seems to be rather short, it remains to be assessed whether or not this may be beneficial to regenerative processes in vivo or not. A number of studies have already evaluated the advantages of using ASPCs in combination with fibrin glue in a variety of cell‐based regeneration in multiple tissue types in animal models, for example, for the creation of skin tissue (Ozpur et al, ), rat acute myocardial infarction (Chen et al, ), cartilage regeneration (Wei et al, ), and support of peripheral nerve healing (Carriel et al, ; Di Summa et al, ; Reichenberger et al, ). All studies imply some positive effect that in our eyes need further evaluation given our findings in this study.…”

Section: Discussionmentioning

confidence: 99%

Fibrin glue displays promising in vitro characteristics as a potential carrier of adipose progenitor cells for tissue regeneration

Krug

Beer

Hartmann

et al. 2019

J Tissue Eng Regen Med

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Adipose-derived multipotent stem/progenitor cells (ASPCs) were shown to be ideal candidates for cell-based regenerative therapies. Yet, despite their huge potential, successful clinical applications are still rare. It was suggested that the efficacy of ASPCs at the recipient site depends on the vehicle of cell delivery. In this study, for preparation of a murine critical-size nerve defect model, we assessed the commercially available fibrin gel (ARTISS) as a potential cell carrier.In a thorough in vitro analysis, we investigated cell-fibrin interactions and analyzed the distribution and the long-term behavior of ASPCs cultivated in fibrin gel under normoxic and hypoxic conditions. ASPCs attached to the surface of a thin fibrin layer (two-dimensional condition) and spread with the abundant formation of actin stress fibers. Cells cultured within a fibrin matrix (three-dimensional condition) displayed a uniform distribution and formed interconnected networks while exhibiting strong cell-matrix interactions. Using time-lapse analysis, cells were found to migrate out of the gel and subsequently proliferated robustly both under hypoxic and normoxic conditions. During 14 days of culture in fibrin gel, ASPCs showed high viability, metabolic, and remodeling activities. At the end of the culture period, the fibrin matrix was degraded entirely accompanied by an upregulation of matrix metalloproteinases.In conclusion, fibrin gel stands out as a valuable biomaterial for delivering vital and active cells to damaged tissues. As a direct proof, ASPCs carried in a fibrin matrix will be evaluated in a murine critically sized peripheral nerve repair model. KEYWORDS adipose-derived progenitor cell, ASPC, fibrin glue, mesenchymal stem cell, MSC, regenerative medicine

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Section: Discussionmentioning

confidence: 99%

Fibrin glue displays promising in vitro characteristics as a potential carrier of adipose progenitor cells for tissue regeneration

Krug

Beer

Hartmann

et al. 2019

J Tissue Eng Regen Med

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“…In the future, the authors plan to develop hybrid artificial nerve conduits by infusing SVF into biodegradable conduits and investigate the clinical validity of this method. In addition, Reichenberger et al report ASCs embedded in fibrin matrix can significantly enhance nerve regeneration progress after sciatic nerve transection and direct epineural suture repair . Similarly, SVF cells have a possibility for improving nerve regeneration applied to epineural suture repair at the autologous nerve grafting or simple nerve repair.…”

Section: Discussionmentioning

confidence: 99%

Facial‐nerve regeneration ability of a hybrid artificial nerve conduit containing uncultured adipose‐derived stromal vascular fraction: An experimental study

et al. 2016

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“…A few recent publications related to the use of endogenous biomaterials applied to PNR can be found in Table 6 [164][165][166][167][168][169][170][171][172][173][174]. Furthermore, Fig.…”

Section: Alginatementioning

confidence: 99%

Fundamentals and Current Strategies for Peripheral Nerve Repair and Regeneration

Carvalho

Reis

Oliveira

2020

Advances in Experimental Medicine and Biology

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A body of evidence indicates that peripheral nerves have an extraordinary yet limited capacity to regenerate after an injury. Peripheral nerve injuries have confounded professionals in this field, from neuroscientists to neurologists, plastic surgeons, and the scientific community. Despite all the efforts, full functional recovery is still seldom. The inadequate results attained with the "gold standard" autograft procedure still encourage a dynamic and energetic research around the world for establishing good performing tissue engineered alternative grafts. Resourcing to nerve guidance conduits, a variety of methods have been experimentally used to bridge peripheral nerve gaps of limited size, up to 30-40 mm in length, in humans. Herein, we aim to summarize the fundamentals related to peripheral nerve anatomy and overview the challenges and scientific evidences related to peripheral nerve injury and repair mechanisms. The most relevant reports dealing with the use of both synthetic and naturalbased biomaterials used in tissue engineering strategies when treatment of nerve injuries is envisioned are also discussed in depth, along with the state-of-the-art approaches in this field.

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ADSCs in a fibrin matrix enhance nerve regeneration after epineural suturing in a rat model

Abstract: ADSCs embedded in a fibrin matrix can significantly enhance regeneration of peripheral nerve injuries after primary coaptation. © 2015 Wiley Periodicals, Inc. Microsurgery 36:491-500, 2016.

Cited by 40 publications

References 49 publications

Fibrin glue displays promising in vitro characteristics as a potential carrier of adipose progenitor cells for tissue regeneration

Fibrin glue displays promising in vitro characteristics as a potential carrier of adipose progenitor cells for tissue regeneration

Facial‐nerve regeneration ability of a hybrid artificial nerve conduit containing uncultured adipose‐derived stromal vascular fraction: An experimental study

Fundamentals and Current Strategies for Peripheral Nerve Repair and Regeneration

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