Adsorbents with a Covalently Bonded Polymer Layer for Hydrophilic Interaction Liquid Chromatography

A comparison of 4 amino phases representing 3-aminopropyl silica batches with different amount of grafted functional groups was made. To evaluate the chromatographic properties of the adsorbents, the Tanaka test for hydrophilic stationary phases was used, and the retention of polar substances of various classes in HILIC mode was also studied. It is shown that even small changes in nitrogen content between different batches of 3-aminopropyl silica have a significant effect on the retention of polar analytes. Hydrophilicity of a substrate was shown to have the greatest effect on its chromatographic behavior, and its evaluation using the Tanaka test is the basis for a batch selection either for separation of particular classes of polar substances or for further modification aiming at obtaining new phases.

show abstract

Chromatographic Comparison of HILIC Stationary Phases Prepared Using Various Hydrophilization Techniques

Chernobrovkina

2023

LCGC N. Am.

View full text Add to dashboard Cite

Selectivity of hydrophilic interaction liquid chromatography (HILIC) stationary phases is crucial for choosing a column for separating target polar compounds, which is why it is important to know the effect of each functional group on the chromatographic properties of the material. Revealing the trends in selectivity changes with variations of adsorbent structure at different stages of preparation can help to regulate the interactions of the stationary phase with analytes and create novel materials with improved performance. This article compares several silica-based HILIC stationary phases with different types of coatings, including polar polymers, antibiotic macromolecules, zwitterionic, and amide groups. Synthesized adsorbents were characterized and compared using the Tanaka test for hydrophilic stationary phases. Their chromatographic performance was evaluated using model mixtures of neutral, zwitterionic, and negatively and positively charged polar analytes. The obtained results provided better understanding of the factors affecting stationary phase selectivity.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Adsorbents with a Covalently Bonded Polymer Layer for Hydrophilic Interaction Liquid Chromatography

Cited by 3 publications

References 30 publications

Comparison of the Properties of 3-Aminopropyl Silica with Different Nitrogen Content in HILIC Mode

Comparison of the Properties of 3-Aminopropyl Silica with Different Nitrogen Content in HILIC Mode

Comparison of the Properties of 3-Aminopropyl Silica With Different Nitrogen Content in Hilic Mode

Chromatographic Comparison of HILIC Stationary Phases Prepared Using Various Hydrophilization Techniques

Contact Info

Product

Resources

About