Adsorption and desorption of cellulase on/from lignin pretreated by dilute acid with different severities

Wang, Jin‐Ye; Hao, Xixun; Wen, Peiyao; Tian, Zhigang; Zhang, Junhua

doi:10.1016/j.indcrop.2020.112309

Cited by 32 publications

(13 citation statements)

References 53 publications

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“…Hence, it can be concluded that hydrophobic interactions operating between lignin and cellulase during enzymatic hydrolysis inhibit enzymatic conversion. Similar conclusions were also drawn in the work of Wang et al [ 47 ]. Overall, these SPR results indicate that the method can be used successfully to investigate mechanisms of interactions between lignin and enzymes.…”

Section: Resultssupporting

confidence: 91%

Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

Lin

Yang

Zheng

et al. 2021

Biotechnol Biofuels

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Background During the dilute acid pretreatment process, the resulting pseudo-lignin and lignin droplets deposited on the surface of lignocellulose and inhibit the enzymatic digestibility of cellulose in lignocellulose. However, how these lignins interact with cellulase enzymes and then affect enzymatic hydrolysis is still unknown. In this work, different fractions of surface lignin (SL) obtained from dilute acid-pretreated bamboo residues (DAP-BR) were extracted by various organic reagents and the residual lignin in extracted DAP-BR was obtained by the milled wood lignin (MWL) method. All of the lignin fractions obtained from DAP-BR were used to investigate the mechanism for interaction between lignin and cellulase using surface plasmon resonance (SPR) technology to understand how they affect enzymatic hydrolysis Results The results showed that removing surface lignin significantly decreased the yield for enzymatic hydrolysis DAP-BR from 36.5% to 18.6%. The addition of MWL samples to Avicel inhibited its enzymatic hydrolysis, while different SL samples showed slight increases in enzymatic digestibility. Due to the higher molecular weight and hydrophobicity of MWL samples versus SL samples, a stronger affinity for MWL (KD = 6.8–24.7 nM) was found versus that of SL (KD = 39.4–52.6 nM) by SPR analysis. The affinity constants of all tested lignins exhibited good correlations (r > 0.6) with the effects on enzymatic digestibility of extracted DAP-BR and Avicel. Conclusions This work revealed that the surface lignin on DAP-BR is necessary for maintaining enzyme digestibility levels, and its removal has a negative impact on substrate digestibility.

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Section: Resultssupporting

confidence: 91%

Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

Lin

Yang

Zheng

et al. 2021

Biotechnol Biofuels

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“…After AC hydrolysis, the maximum adsorption capacity of AC-CELs increased to 112.4 mg/g (160-CEL), 102.0 mg/g (170-CEL), and 152.5 mg/g (180-CEL). This trend was consistent with the result in previous reports that acid pretreatment can increase the maximum adsorption capacity of lignin . 180-CEL exhibited the highest maximum adsorption capacity, which indicated that lignin in 5% AC-hydrolyzed poplar at 180 °C might have the strongest nonproductive adsorption …”

Section: Resultssupporting

confidence: 92%

“…This result could be because enzymatic hydrolysis increased the lignin content of CEL samples . Normally, acid pretreatment could decrease the hydrophilic group and increase the hydrophobicity of lignin. , Interestingly, in this work, the hydrophobicities of AC-CELs (1.8–2.5 L/g) were lower than that of BM-CEL (4.6 L/g), which could be because AC hydrolysis can increase the hydrophilic groups (such as −OH and −COOH) of lignin in the substrate…”

Section: Resultsmentioning

confidence: 57%

Effect of Dilute Acetic Acid Hydrolysis on Xylooligosaccharide Production and the Inhibitory Effect of Cellulolytic Enzyme Lignin from Poplar

Wen

Zhang

Wei

et al. 2021

ACS Sustainable Chem. Eng.

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Acetic acid (AC) hydrolysis has been reported to prepare xylooligosaccharides (XOS) from poplar. However, the influence of AC hydrolysis on the lignin structure changes is not clear, which is important for the following enzymatic hydrolysis of poplar. Herein, AC was used to produce XOS, and cellulase adsorption on cellulolytic enzyme lignin (CEL) from AC-hydrolyzed poplar and its inhibitory effect on two commercial cellulase preparations were investigated. AC hydrolysis gave a XOS yield of 39.8% from poplar. After AC hydrolysis at 170 °C, the hydrophobicity and ζ-potential of CEL decreased to 2.3 L/g and 14.8 mV, respectively. The adsorption strength of CTec2 on CEL samples did not increase by AC hydrolysis, and the inhibitory effect of CEL on Celluclast 1.5L and β-glucosidase was observed, but not on CTec2. CEL samples improved the lytic polysaccharide monooxygenase (LPMO) activity of the enzymatic hydrolysis by CTec2. After CEL samples were added in enzymatic hydrolysis, the free filter paper activity of Celluclast 1.5L and β-G retained in the enzymatic hydrolysate decreased from 60.5 to 29.3−42.9%. The addition of CEL samples in enzymatic hydrolysis could not decrease the free filter paper activity of CTec2 retained in the enzymatic hydrolysate. In the enzymatic hydrolysis with CEL samples, higher glucose yields were obtained by CTec2 than those by Celluclast 1.5L and β-glucosidase. This work will help to understand the structure and inhibitory effects of AC-CELs and guide the development of AC hydrolysis for the production of XOS and monosaccharides from poplar.

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“…3), which was consistent with the previous reports (Sun et al 2016;Huang et al 2017). It is well understood that the lignins (OL-EH and OL-LP) with higher enzyme distribution coefficient (R) resulted in the inhibitory effects on enzymatic hydrolysis, potentially by reducing the enzymes activity and movability (Wang et al 2020). However, the reasons why the lignins (OL-CW, OL-BW, and OL-AS) with lower enzyme distribution coefficient showed positive effects on enzymatic hydrolysis of pure cellulose are difficult to explain.…”

Section: Enzyme Adsorption On Organosolv Ligninssupporting

confidence: 91%

Organosolv lignin properties and their effects on enzymatic hydrolysis

Huang

Lai

Sun

et al. 2020

BioRes

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Lignin plays a crucial role in enzymatic hydrolysis of lignocellulosic biomass. To evaluate the correlation between lignin properties and its effects on enzymatic hydrolysis, five organosolv lignins (OLs) were isolated from woody biomass, and their physico-chemical properties and structural features were characterized. The effects of OL addition on enzymatic hydrolysis of microcrystalline cellulose (pure cellulose) were assessed first, which showed their disparate effects. The addition of three OLs increased the 72 h hydrolysis yield by 7.4% to 10.1%, while the addition of other two OLs reduced the 72 h hydrolysis yield by 3.2% to 20.4%. A strong correlation between the enzyme distribution coefficient on lignins and the 72 h hydrolysis yields indicated that the enzyme-lignin interaction played a significant role in determining the lignin effects. More importantly, a correlation between lignin properties (hydrophobicity, zeta potential, and particle size) and the enzyme distribution coefficient was established. Identifying the key lignin properties will give insights to reduce the lignin inhibition by altering the lignin properties, thereby promoting enzymatic hydrolysis of lignocellulose.

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Adsorption and desorption of cellulase on/from lignin pretreated by dilute acid with different severities

Cited by 32 publications

References 53 publications

Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

Effect of Dilute Acetic Acid Hydrolysis on Xylooligosaccharide Production and the Inhibitory Effect of Cellulolytic Enzyme Lignin from Poplar

Organosolv lignin properties and their effects on enzymatic hydrolysis

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