Adsorption and desorption of pentachlorophenol on cells ofMycobacterium chlorophenolicum PCP-1

Brandt, S; Zeng, An‐Ping; Deckwer, Wolf‐Dieter

doi:10.1002/(sici)1097-0290(19970805)55:3<480::aid-bit3>3.0.co;2-8

Cited by 57 publications

(25 citation statements)

References 26 publications

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“…From this information, we calculated that at an equilibrium concentration of 3.76 μM PCP, approximately 0.75 μmol of PCP per 1 g −1 of biomass was adsorbed to the cells. Corresponding data for the Gram‐positive bacterium Mycobacterium chlorophenolicum PCP‐1 are approximately 85 μmol of PCP adsorbed per gram of bio‐mass at an equilibrium concentration of 175 μM [12]. Sinclair et al [26] reported a major effect of pH on the adsorption of 2,4‐DCP by lux ‐modified Escherichia coli cells that was attributable to changes in the predominance of nonionized and 2,4‐dichlorophenolate species.…”

Section: Discussionmentioning

confidence: 99%

“…In this latter study, however, it was ultimately concluded that an undetermined, micro‐organism–specific process enhanced substrate desorption to the aqueous phase before mineralization. Biosorption of organic contaminants onto bacterial surfaces may occur as well [10–12]. Thus, the presence of bacterial cells in soil‐contaminant systems may, either passively or actively, alter the distribution of a contaminant between the sorbed and the aqueous phase and, therefore, affect the bioavailability of the target compound.…”

Section: Introductionmentioning

confidence: 99%

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Interactions between soil, toxicant, and a lux‐marked bacterium during solid phase–contact toxicity testing

Shaw¹,

Beaton

Glover

et al. 2000

Enviro Toxic and Chemistry

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Bioluminescence‐based, solid‐contact toxicityassaysallow test bacterium and toxicant tointeract atthesolid‐solution interface. A lux‐marked bacterium, Burkholderia sp. RASC, and 2,4‐dichlorophenol (2,4‐DCP) were used to characterize these interactions. In the basic bioassay, cells were added to soil slurries containing 2,4‐DCP (0–120 μg ml−1). After 15 min, soil was removed by centrifugation, and bioluminescence in the supernatant was determined. Investigation of 2,4‐DCP adsorption to soil revealed that sorption was linear and not significantly (p > 0.1) affected by the presence of Burkholderia cells. The numbers of culturable Burkholderia cells in the assay supernatant were 48.2 to 64.8% of the inoculum and independent of the soil weight. The effect of soil on 2,4‐DCP toxicity was investigated by comparing soil aqueous extract and contact assays. The percentage bioluminescence for the contact assay was consistently higher than the extract assay at all test concentrations, and counts of viable Burkholderia cells were enhanced by the presence of 2,4‐DCP in the contact assay. Expressing results as specific bioluminescence decreased the variability in response and the discrepancy in results between the two protocols. We suggest that solid‐contact assays need improvement to ensure defined contact between cells and solid phase, and that the reporting of specific activity should be emphasized.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Interactions between soil, toxicant, and a lux‐marked bacterium during solid phase–contact toxicity testing

Shaw¹,

Beaton

Glover

et al. 2000

Enviro Toxic and Chemistry

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show abstract

“…Other sorption materials have been already suggested for PCP sorption in the literature, e.g. inactivated biomass, peat, activated sludge, peat:bentonite mixture, chitosan, and pine bark (Bell and Tsezos 1987;Tanjore and Viraraghavan 1997;Brandt et al 1997;Viraraghavan and Slough 1999;Zheng et al 2004;Jianlong et al 2000;Brás et al 2005).…”

Section: Adsorption/desorption Of Pcp On/from Lignitementioning

confidence: 99%

Biodegradation and ecotoxicity of soil contaminated by pentachlorophenol applying bioaugmentation and addition of sorbents

Sejáková

Dercová

Tóthová

2008

World J Microbiol Biotechnol

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Biodegradation of pentachlorophenol (PCP) in soil by autochthonous microorganisms and in soil bioaugmented by the bacterial strain Comamonas testosteroni CCM 7530 was studied. Subsequent addition of organomineral complex (OMC) or lignite as possible sorbents for PCP immobilization has been investigated as well. The OMC was prepared from humic acids (HAs) isolated from lignite by binding them onto zeolite. Biodegradation of PCP and number of colony forming units (CFUs) were determined in the three types of soil, Chernozem, Fluvisol, and Regosol, freshly spiked with PCP and amended separately with tested sorbents. The enhancing effect of sorbent addition and bioaugmentation on PCP biodegradation depended mainly on the soil type and the initial PCP concentration. Microbial activity resulted in biotransformation of PCP into certain toxic substances, probably lower chlorinated phenols that are more soluble than PCP, and therefore more toxic to present biota. Therefore, it was necessary to monitor soil ecotoxicity during biodegradation. Addition of the OMC resulted in a more significant decrease of soil toxicity in comparison with addition of lignite. Lignite and OMC appear to be good traps for PCP with potential application in remediation technology.

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“…Traditionally, adsorption by activated carbon was the most efficient method for the removal of phenolic compounds from water. Recently, microorganisms have been considered promising biosorbents of phenols (Brandt et al 1997). Various sorption processes may be involved: adsorption, ion exchange and covalent bonding (Fourest and Volesky 1997).…”

Section: Introductionmentioning

confidence: 99%

Partial pyrolysis of olive wood to improve its sorption of chlorophenols and nitrophenols

El‐Sheikh

2013

Int. J. Environ. Sci. Technol.

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Partial pyrolysis alters the chemical and textural properties of the lignocellulosic material. This work reports the effect of partial pyrolysis of olive wood on adsorption isotherms, kinetics and thermodynamics of chloro and nitrophenols. Shape of adsorption isotherms of the partially pyrolyzed sorbents was L3 for phenol; L2 for 2-nitrophenol and 2,4-dinitrophenol; H3 for 2-chlorophenol, 3-chlorophenol and 4-nitrophenol; and H2 for 4-chlorophenol. The pyrolyzed olive wood sorbents obeyed Langmuir and Freundlich models. Pyrolysis raised adsorption capacity, favorability and spontaneity; the adsorption became more exothermic; the randomness decreased. The adsorption was mainly physical; it occurred first by film diffusion then by pore-filling. Adsorption followed second-order rate kinetics. Adsorption of phenols on olive wood seemed to be governed by hydrophobic interaction. Washing the pyrolyzed olive wood with ethanol caused a decrease in adsorption capacity, favorability and spontaneity, and the adsorption became less exothermic. This indicated that pyrolysis produced species on the olive wood surface that played a significant role in phenols adsorption.

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Adsorption and desorption of pentachlorophenol on cells ofMycobacterium chlorophenolicum PCP-1

Cited by 57 publications

References 26 publications

Interactions between soil, toxicant, and a lux‐marked bacterium during solid phase–contact toxicity testing

Interactions between soil, toxicant, and a lux‐marked bacterium during solid phase–contact toxicity testing

Biodegradation and ecotoxicity of soil contaminated by pentachlorophenol applying bioaugmentation and addition of sorbents

Partial pyrolysis of olive wood to improve its sorption of chlorophenols and nitrophenols

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