Adsorption and separation of HCV particles by novel affinity aptamer-functionalized adsorbents

Chen, Bin; Ye, Qing; Zhou, Kangping; Wang, Yefu

doi:10.1016/j.jchromb.2016.03.004

Cited by 10 publications

(7 citation statements)

References 33 publications

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“…The glass microbeads were first amino-silane modified and then treated with phenylene diisothiocyanate (PDITC) to generate the isothiocyanate-modified bead, in which the active isothiocyanate group can react with the amine-functionalized anti E2 aptamer. Chen et al [ 35 ] have fabricated a hepatitis C virus- (HCV-) specific aptamer conjugated with amine to react with the sepharose 4 Fast Flow (4FF) beads to immobilize the aptamer. The carboxylated-derivative sepharose 4FF beads, which is 4% highly cross linked spherical sepharose is activated via NHS/EDC prior to peptide bond formation with the amine-terminated aptamers ( Figure 1(a) ).…”

Section: Functionalization Of Aptamers For Biodecontaminationmentioning

confidence: 99%

“…High specificity conferred by aptamers with minimal off-target binding accounts for its wide application in the fields of drug delivery, therapeutic agent, molecular biosensing, and biomimetic engineering [ 17 , 65 – 70 ]. This also strongly insinuates that aptamers can also be potentially used to decontaminate contaminants in vivo [ 27 , 35 ].…”

Section: Aptamers As the Potential In Vivo Decomentioning

confidence: 99%

“…Aptamer-based decontamination assay is used to selectively capture and detoxify infectious agents. In one study, Chen et al[ 35 ] used HCV-specific single-stranded DNA aptamers to decontaminate approximately 80% of the HCV from human plasma samples. This novel apta-decontamination procedure could effectively remove HCV particles and likely to serve as a novel therapy option for HCV.…”

Section: Aptamers As the Potential In Vivo Decomentioning

confidence: 99%

“… (a) Schematic representation of the conjugation of the amine-terminated aptamer on the surface of the carboxylated-functionalized sepharose 4FF beads via NHS/EDC activation [ 35 ]. (b) Conjugation of the amine-functionalized aptamer on the surface of nanoparticles that contain a stable poly (lactic acid)-polyethylene glycol (PLA-PEG) block copolymer with a terminal carboxylic acid functional group (PLA-PEG-COOH) [ 37 ].…”

Section: Figurementioning

confidence: 99%

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Aptamers as the Agent in Decontamination Assays (Apta-Decontamination Assays): From the Environment to the Potential Application In Vivo

Bilibana

Citartan

Yeoh

et al. 2017

Journal of Nucleic Acids

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The binding specificity and affinity of aptamers have long been harnessed as the key elements in the development of aptamer-based assays, particularly aptasensing application. One promising avenue that is currently explored based on the specificity and affinity of aptamers is the application of aptamers in the decontamination assays. Aptamers have been successfully harnessed as the decontamination agents to remove contaminants from the environment and to decontaminate infectious elements. The reversible denaturation property inherent in aptamers enables the repeated usage of aptamers, which can immensely save the cost of decontamination. Analogous to the point-of-care diagnostics, there is no doubt that aptamers can also be deployed in the point-of-care aptamer-based decontamination assay, whereby decontamination can be performed anywhere and anytime for instantaneous decision-making. It is also prophesied that aptamers can also serve more than as a decontaminant, probably as a tool to capture and kill hazardous elements, particularly pathogenic agents.

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Section: Functionalization Of Aptamers For Biodecontaminationmentioning

confidence: 99%

Section: Aptamers As the Potential In Vivo Decomentioning

confidence: 99%

Section: Aptamers As the Potential In Vivo Decomentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

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Aptamers as the Agent in Decontamination Assays (Apta-Decontamination Assays): From the Environment to the Potential Application In Vivo

Bilibana

Citartan

Yeoh

et al. 2017

Journal of Nucleic Acids

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“…Transmission and scanning electron microscopy of fouled resin beads clearly showed irreversible containment accumulation 6 14 31 43 . Although these studies were very informative, they were performed on dried resin beads, bearing little resemblance to the hydrated gel 44 . Direct measurement of hydrated resin is required to gain more detailed insights into fouling.…”

mentioning

confidence: 99%

In-column ATR-FTIR spectroscopy to monitor affinity chromatography purification of monoclonal antibodies

Boulet-Audet

Kazarian

Byrne

2016

Sci Rep

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In recent years many monoclonal antibodies (mAb) have entered the biotherapeutics market, offering new treatments for chronic and life-threatening diseases. Protein A resin captures monoclonal antibody (mAb) effectively, but the binding capacity decays over repeated purification cycles. On an industrial scale, replacing fouled Protein A affinity chromatography resin accounts for a large proportion of the raw material cost. Cleaning-in-place (CIP) procedures were developed to extend Protein A resin lifespan, but chromatograms cannot reliably quantify any remaining contaminants over repeated cycles. To study resin fouling in situ, we coupled affinity chromatography and Fourier transform infrared (FTIR) spectroscopy for the first time, by embedding an attenuated total reflection (ATR) sensor inside a micro-scale column while measuring the UV 280 nm and conductivity. Our approach quantified the in-column protein concentration in the resin bed and determined protein conformation. Our results show that Protein A ligand leached during CIP. We also found that host cell proteins bound to the Protein A resin even more strongly than mAbs and that typical CIP conditions do not remove all fouling contaminants. The insights derived from in-column ATR-FTIR spectroscopic monitoring could contribute to mAb purification quality assurance as well as guide the development of more effective CIP conditions to optimise resin lifespan.

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Aptamer-Based Affinity Chromatography for Protein Extraction and Purification

Perret

Boschetti²

2019

Advances in Biochemical Engineering/Biotechnology

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Adsorption and separation of HCV particles by novel affinity aptamer-functionalized adsorbents

Cited by 10 publications

References 33 publications

Aptamers as the Agent in Decontamination Assays (Apta-Decontamination Assays): From the Environment to the Potential Application In Vivo

Aptamers as the Agent in Decontamination Assays (Apta-Decontamination Assays): From the Environment to the Potential Application In Vivo

In-column ATR-FTIR spectroscopy to monitor affinity chromatography purification of monoclonal antibodies

Aptamer-Based Affinity Chromatography for Protein Extraction and Purification

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