Adsorption at Water–Air and Water–Organic Liquid Interfaces.

Bartell, F. E.; Davis, James K.

doi:10.1021/j150413a019

Cited by 32 publications

(15 citation statements)

References 10 publications

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“…The increase in area per molecule occurs because an excess of alcohol molecules at the interface facilitates alcohol partitioning into the monolayer. Previous studies have shown a surface excess of alcohols at interfacial alkane monolayers by experiment 30 and computer simulation. 31 Extension of this work to water/lipid interfaces has shown similar excesses, albeit not as large.…”

Section: Resultsmentioning

confidence: 96%

Short-Chain Alcohols Promote Accelerated Membrane Distention in a Dynamic Liposome Model of Exocytosis

et al. 2008

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We have used amperometric measurements in a model system consisting of two liposomes connected with a membrane nanotube to monitor catechol release during artificial exocytosis and thereby to elucidate the effect of small-chain alcohols on this dynamic membrane process. To determine the rate of membrane shape change, catechol release during membrane distention was monitored amperometrically, and the presence of alcohols in the buffer was shown to accelerate the membrane distention process in a concentration-dependent manner. Compression isotherms for the same lipid composition in the absence and presence of ethanol and 1-propanol were measured to determine how these short-chain alcohols affect the lipid packing in monolayers. The isotherms show a marked decrease in lipid packing density that is dependent on the particular alcohol and its concentration. Comparison of the electrochemical and isotherm results suggests a correlation between decreasing lipid packing density and increasing rates of membrane shape change.

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Section: Resultsmentioning

confidence: 96%

Short-Chain Alcohols Promote Accelerated Membrane Distention in a Dynamic Liposome Model of Exocytosis

et al. 2008

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“…10 A show the g-values versus alcohol concentration we obtained from applying the Rawicz's model (g ¼ K A /6) to our average K A data for SOPC membranes. For comparison, g-values of alkane-water/alcohol interfaces from the data of Bartell and Davis (1941) and Rivera et al (2003) are plotted alongside. In comparison to the alkane g-values, the bilayer g-values decrease much less as alcohol concentration is increased.…”

Section: Bilayer Interfacial Tension Area Per Headgroup and Membranmentioning

confidence: 99%

“…11, we can FIGURE 10 (A) Interfacial tension, g, values versus alcohol concentration for the four alcohol/water mixtures: methanol (diamonds), ethanol (squares), propanol (triangles), and butanol (circles). Values at the SOPC bilayer-water interface (solid marks) are from the K A /6 relation, and values at the alkane-water interface (open marks) are reprinted with permission from Bartell and Davis (1941) (Copyright 1941 American Chemical Society) and Rivera et al (2003) overlay our g-data onto a fit of P versus area per molecule data for an SOPC monolayer at the air-water interface from the works of Smaby et al (1994) and obtain estimates for the area-per-molecule values. For all four types of alcohol molecules, we have plotted the g-values onto the SOPCwater monolayer isotherm to obtain area-per-molecule estimates instead of using an SOPC-alcohol-water monolayer isotherm.…”

Section: Bilayer Interfacial Tension Area Per Headgroup and Membranmentioning

confidence: 99%

“…) We evaluated the derivative dg/da for the SOPC bilayer by successfully fitting g-values versus activity to exponential functions. The total alcohol surface density values (surface excess density plus surface bulkphase density) for the bilayer-water interface (and alkanewater interface for comparison; Bartell and Davis, 1941;Rivera et al, 2003) are presented in Fig. 13 as functions of acyl chain-length and concentration of the alcohol.…”

Section: Surface Excessmentioning

confidence: 99%

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The Influence of Short-Chain Alcohols on Interfacial Tension, Mechanical Properties, Area/Molecule, and Permeability of Fluid Lipid Bilayers

Longo

2004

Biophysical Journal

281

295

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We used micropipette aspiration to directly measure the area compressibility modulus, bending modulus, lysis tension, lysis strain, and area expansion of fluid phase 1-stearoyl, 2-oleoyl phosphatidylcholine (SOPC) lipid bilayers exposed to aqueous solutions of short-chain alcohols at alcohol concentrations ranging from 0.1 to 9.8 M. The order of effectiveness in decreasing mechanical properties and increasing area per molecule was butanol>propanol>ethanol>methanol, although the lysis strain was invariant to alcohol chain-length. Quantitatively, the trend in area compressibility modulus follows Traube's rule of interfacial tension reduction, i.e., for each additional alcohol CH(2) group, the concentration required to reach the same area compressibility modulus was reduced roughly by a factor of 3. We convert our area compressibility data into interfacial tension values to: confirm that Traube's rule is followed for bilayers; show that alcohols decrease the interfacial tension of bilayer-water interfaces less effectively than oil-water interfaces; determine the partition coefficients and standard Gibbs adsorption energy per CH(2) group for adsorption of alcohol into the lipid headgroup region; and predict the increase in area per headgroup as well as the critical radius and line tension of a membrane pore for each concentration and chain-length of alcohol. The area expansion predictions were confirmed by direct measurements of the area expansion of vesicles exposed to flowing alcohol solutions. These measurements were fitted to a membrane kinetic model to find membrane permeability coefficients of short-chain alcohols. Taken together, the evidence presented here supports a view that alcohol partitioning into the bilayer headgroup region, with enhanced partitioning as the chain-length of the alcohol increases, results in chain-length-dependent interfacial tension reduction with concomitant chain-length-dependent reduction in mechanical moduli and membrane thickness.

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“…For the semistalk with a hydrophobic patch of ϳ3 nm diameter (corresponding to that of the narrowest part of the stalk), F O/W can be ϳ80kT, i.e., it can be comparable to the elastic energy of a stalk (Chernomordik et al, 1995b). At the hemifusion-promoting concentration of 0.5 M, ethanol decreases the water/n-heptane interface tension from 50 to 40 dyne/cm (Bartell and Davis, 1941). For a 3-nm hydrophobic patch, the alcohol-induced decrease in interfacial tension lowers F O/W from ϳ80kT to 64kT and thus might notably facilitate breaking of the lipid monolayer required for semistalk formation.…”

Section: Alcohol Can Facilitate a Local Breaking Of Lipid Monolayer Continuity Required For Stalk Formationmentioning

confidence: 99%

Short-Chain Alcohols Promote an Early Stage of Membrane Hemifusion

Chanturiya

Leikina

Zimmerberg

et al. 1999

Biophysical Journal

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Hemifusion, the linkage of contacting lipid monolayers of two membranes before the opening of a fusion pore, is hypothesized to proceed through the formation of a stalk intermediate, a local and strongly bent connection between membranes. When the monolayers' propensity to bend does not support the stalk (e.g., as it is when lysophosphatidylcholine is added), hemifusion is inhibited. In contrast, short-chain alcohols, reported to affect monolayer bending in a manner similar to that of lysophosphatidylcholine, were here found to promote hemifusion between fluorescently labeled liposomes and planar lipid bilayers. Single hemifusion events were detected by fluorescence microscopy. Methanol or ethanol (1.2-1.6 w/w %) added to the same compartment of the planar bilayer chamber as liposomes caused a 5-50 times increase in the number of hemifusion events. Alcohol-induced hemifusion was inhibited by lysophosphatidylcholine. Promotion of membrane hemifusion by short-chain alcohol was also observed for cell-cell fusion mediated by influenza virus hemagglutinin (HA). Alcohol promoted a fusion stage subsequent to the low pH-dependent activation of HA. We propose that binding of short-chain alcohol to the surface of membranes promotes hemifusion by facilitating the transient breakage of the continuity of each of the contacting monolayers, which is required for their subsequent merger in the stalk intermediate.

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Adsorption at Water–Air and Water–Organic Liquid Interfaces.

Cited by 32 publications

References 10 publications

Short-Chain Alcohols Promote Accelerated Membrane Distention in a Dynamic Liposome Model of Exocytosis

Short-Chain Alcohols Promote Accelerated Membrane Distention in a Dynamic Liposome Model of Exocytosis

The Influence of Short-Chain Alcohols on Interfacial Tension, Mechanical Properties, Area/Molecule, and Permeability of Fluid Lipid Bilayers

Short-Chain Alcohols Promote an Early Stage of Membrane Hemifusion

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