Adsorption behavior of antimicrobial peptide histatin 5 on PMMA

Abstract

Adsorption of antimicrobial peptide histatin 5 on a poly(methyl methacrylate) denture base may serve to prevent biofilm formation, leading to a reduction of denture-induced stomatitis. This study focused on adsorption behavior of histatin 5 onto PMMA surfaces modified using a cold plasma technique and the effectiveness of histatin 5 adsorption for reducing C. albicans biofilm formation by the quartz crystal microbalance-dissipation (QCM-D) technique. PMMA spin-coated specimens were treated with oxygen (O 2 ) plasma using a plasma surface modification apparatus. The amount of histatin 5 adsorbed onto the PMMA treated with O 2 plasma is more than six times greater than that adsorbed onto untreated PMMA. The degree of histatin 5 adsorption had a negative correlation with the contact angle, whereas that of zeta-potential showed no significant correlation. XPS analysis revealed that the introduction of the carboxyl and O 2 functional groups were observable on the O 2 plasma treated

Prevention of biofilm formation on titanium surfaces modified with conjugated molecules comprised of antimicrobial and titanium-binding peptides

“…Many studies have reported that histatin 5 exhibited antimicrobial activity in the treatment of oral fungal infections such as C. albicans (Edgerton et al,1995 ;Kavanagh et al, 2001;Yoshinari et al, 2006). In this study, minTBP1-His 5 exhibited antimicrobial activity against P. gingivalis.…”

“…These antimicrobial activities are believed to regulate biofilm formation in the oral cavity, and have fungicidal and fungistatic effects on Candida albicans cells, in particular (Edgerton et al, 1995;Ruissen et al, 1999). In a previous study, we found that C. albicans colonization on poly(methyl methacrylate) was reduced significantly by adsorption of histatin 5 (Yoshinari et al, 2006). In addition, histatin 5 may play an important role in the regulation of periodontopathic bacteria such as Porphyromonas gingivalis, which is involved in inflammatory periodontal diseases and peri-implantitis (Imatani et al, 2000;Gusman et al, 2001;Kato et al, 2004).…”

Effect of pH and Addition of Salt on the Adsorption Behavior of Lysozyme on Gold, Silica, and Titania Surfaces Observed by Quartz Crystal Microbalance with Dissipation Monitoring

“…The QCM-D method has recently been successfully applied in the field of oral science [12][13][14] . This technique is sometimes used in combination with other methods such as AFM 15) or some optical techniques 14,16) .…”

Development and Validation of an In Vivo Candida albicans Biofilm Denture Model

“…In vitro biofilm models have the advantage of lower cost and are ideal for high-throughput screening assays and antifungal testing (12,56,61,71). These models have provided important phenotypic information regarding drug resistance in Candida biofilms.…”