Adsorption/desorption characteristics and chromatographic purification of polyphenols from Vernonia patula (Dryand.) Merr. using macroporous adsorption resin

Hou, Mengyang; Zhang, Libing

doi:10.1016/j.indcrop.2021.113729

Cited by 39 publications

(24 citation statements)

References 38 publications

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“…The physical properties and manufacturers of eleven resins are shown in Table S2 . Before experimental use, aliquots of adsorbents were leached with ethanol overnight and washed thoroughly with the distilled water as the monomers and porogenic agents in newly bought MARs would affect absorption [ 45 ].…”

Section: Methodsmentioning

confidence: 99%

Purification and Identification of Flavonoid Molecules from Rosa setate x Rosa rugosa Waste Extracts and Evaluation of Antioxidant, Antiproliferative and Antimicrobial Activities

Zhang

et al. 2022

Molecules

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Rosa setate x Rosa rugosa is widely used in the essential oil industry and generates large amounts of waste annually. The purpose of this research is the recycling of bioactive flavonoids from rose waste biomass to develop high-value products. Resin screening and adsorption/desorption dynamic analysis showed that HP20 resin was suitable to purify the flavonoids from R. setate x R. rugosa waste extracts. Under the optimal enrichment process, the product had a 10.7-fold higher purity of flavonoids with a satisfactory recovery of 82.02%. In total, 14 flavonoids were identified in the sample after purification by UHPLC-QTOF-MS. Moreover, the DPPH and ABTS assays revealed that the flavonoids-purified extracts exhibited higher antioxidant activities than the crude extracts. Meanwhile, the purified extracts presented stronger antiproliferative activity against HepG2, Caco-2, MCF-7 and A549 cell lines. The bacteriostatic effects of the purified extracts against four bacteria (Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), Staphylococcus epidermidis (S. epidermidis), Pseudomonas aeruginosa (P. aeruginosa)) and yeast (Candida albicans (C. albicans)) were stronger compared with the crude extracts. It was concluded that flavonoids-enriched extracts from R. setate x R. rugosa waste had the potential to be applied in functional food and pharmaceutical industries.

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Section: Methodsmentioning

confidence: 99%

Purification and Identification of Flavonoid Molecules from Rosa setate x Rosa rugosa Waste Extracts and Evaluation of Antioxidant, Antiproliferative and Antimicrobial Activities

Zhang

et al. 2022

Molecules

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“…Le et al also observed that the adsorption equilibrium of polyphenols (sinapine) from rapeseed meal protein isolate by-products was obtained after 120 min on Amberlite XAD 16 resin [19]. Hou and Zhang reported that polyphenol adsorption equilibrium was reach after 4 h using a highly polar resin (NKA-II) [9]. The surface area of this resin is very close to that of XAD 16 and XAD 4; hence, use of nonpolar or slightly polar resin accelerates the adsorption process.…”

Section: Static Adsorption/desorptionmentioning

confidence: 98%

“…Based on the results, using 5 g of XAD 16 resin, 87.7% of polyphenols was recovered from hazelnut skin extract with a desorption ratio of 92.36% by eluting 10 BV of ethanol solution (70% v/v) as solvent at adsorption and desorption flow rates of 1.5 BV/h. Hou and Zhang recovered 85.74% of total phenol in Vernonia patula extract using NKA-II resin, which is 2.48-fold higher than the total phenol of the crude extract [9]. Vavouraki showed that FPX66 resin absorbed 60% of phenolic compounds from olive mill wastewater and, using a solvent mixture of ethanol/isopropanol (1:1), recovered 70% of polyphenols [28].…”

Section: Dynamic Adsorption/desorptionmentioning

confidence: 99%

“…The concentrations of gallic acid and protocatechuic acid in the purified extract increased by approximately three-fold, whereas catechin, epicatechin, and quercetin were increased by over five-fold compared to those in the crude extract. Hou and Zhang reported that the contents of chlorogenic acid and caffeic acid in V. patula extracts were increased 2.5-fold after column desorption from NKA-II resin using 5.5 BV of 60% ethanol at a flow rate of 3 BV/h [9]. Johnson and Mitchell showed that Amberlite resins assist in the debittering of olives during normal brine storage by adsorbing bitter phenols.…”

Section: Phenolic Content and Antioxidant Activitymentioning

confidence: 99%

“…Although the use of solvents is less costly and simpler, it is not selective and results in diluted extracts with low polyphenol concentrations. Macroporous resins are physiochemically stable polymers with polar, non-polar, or slightly polar characteristics and high adsorption capacities for organic compounds [9]. They can be used to purify and concentrate active compounds from complex extracts [10].…”

Section: Introductionmentioning

confidence: 99%

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Recovery and Concentration of Polyphenols from Roasted Hazelnut Skin Extract Using Macroporous Resins

Zadeh

Zeppa

2022

Foods

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Hazelnut skin is a rich source of polyphenols but is generally discarded during the roasting process of hazelnuts. Previous studies reported the extraction and identification of these compounds using different solvents and procedures; however, there are few reports on their enrichment and purification. In this study, three types of Amber-lite macroporous resins (XAD 16, XAD 4, and XAD 7) were compared to evaluate the enrichment of polyphenols via adsorption and desorption mechanisms. The operating condition parameters for polyphenol adsorption/desorption of each resin were determined, the kinetics of adsorption were examined, and a method for polyphenol recovery was developed using static and dynamic adsorption/desorption. Antioxidant activity and high-performance liquid chromatography-diode array detection were used to confirm the increase in polyphenol obtained using the adsorption/desorption technique. XAD16 showed the highest adsorption capacity, with a recovery of 87.7%, and the adsorption kinetics fit well with a pseudo-second-order model. The highest poly-phenol desorption ratio was observed using an ethanol/water solution (70% v/v) at a flow rate of 1.5 bed volume/h.

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Purification of Polysaccharides from Orostachys cartilaginous Cell Cultures by Macroporous Resin Absorption and Bioactivities of the Purified Polysaccharides

Han

et al. 2022

Chemistry & Biodiversity

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Orostachys cartilaginous is a traditional herbal medicine and its cell cultures contain large amounts of polysaccharides. To utilize the cultured O. cartilaginous cells, this study purified the crude polysaccharides of O. cartilaginous cells by macroporous resin absorption and optimized the purification process in the experiment of orthogonal design with four factors (sample concentration and volume, and eluent concentration and volume) and three levels; the antibacterial and anti‐cancer effects of the purified polysaccharides (OTP) were further examined. The results showed that polysaccharide purity reached 95 % in the optimized group, i. e., 1.6 mg/mL of sample (crude polysaccharides) concentration, 3.0 bed volume (BV) of sample volume, 65 % eluent (ethanol) concentration, and 3.0 BV of eluent volume. In the antibacterial experiment, the growth of three bacterial species, i. e., Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis was inhibited by OTP, whereas that of Escherichia coli was not affected; the largest diameter of the inhibitory zone was found on B. subtilis; the extracellular alkaline phosphatase activity and the electrical conductivity, nucleic acid, and protein levels of B. subtilis increased after OTP treatment, indicating that the inhibition of B. subtilis growth was caused by the leakage of cell contents. In the anti‐cancer experiment, OTP decreased the cell viabilities of the tested human cancer cells, i. e., AGS (gastric cancer), HCT116 (colon cancer), HepG2 (liver cancer), and HeLa (cervical cancer), and the highest inhibitory effect was on HCT116. OTP promoted HCT116 apoptosis and affected the expression of apoptosis‐related proteins, i. e., the expression of B‐cell lymphoma‐2 decreased and that of bcl‐2 associated X protein, cytochrome c, caspase 9 and caspase 3 increased. The findings of the present study suggest that O. cartilaginous cell cultures have a potential application in food or drug production.

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Adsorption/desorption characteristics and chromatographic purification of polyphenols from Vernonia patula (Dryand.) Merr. using macroporous adsorption resin

Cited by 39 publications

References 38 publications

Purification and Identification of Flavonoid Molecules from Rosa setate x Rosa rugosa Waste Extracts and Evaluation of Antioxidant, Antiproliferative and Antimicrobial Activities

Purification and Identification of Flavonoid Molecules from Rosa setate x Rosa rugosa Waste Extracts and Evaluation of Antioxidant, Antiproliferative and Antimicrobial Activities

Recovery and Concentration of Polyphenols from Roasted Hazelnut Skin Extract Using Macroporous Resins

Purification of Polysaccharides from Orostachys cartilaginous Cell Cultures by Macroporous Resin Absorption and Bioactivities of the Purified Polysaccharides

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