Adsorption differences and mechanism of chitooligosaccharides with specific degree of polymerization on macroporous resins with different functional groups

Hou, Yanying; Liu, Lujie; Zhang, Xingxing; Zhu, Yuechao; Qiu, Yongjun; Zhao, Liming

doi:10.1016/j.jiec.2022.08.027

Cited by 6 publications

(1 citation statement)

References 50 publications

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“…Among them, with the increase in pH, the adsorption capacity of XAD-7HP resin on PSKB protein hydrolysates shows an upward trend, reaching the maximum at pH = 7, which is 7.53-11.93 mg/ml. With the continuous increase in pH, the adsorption capacity of XAD-7HP resin on PSKB protein hydrolysates slightly decreases as the adsorption capacity of the resin is related to van der Waals force and hydrogen bond, and its network structure and specific surface area have certain screening performance (24). Under the same pH condition, the adsorption capacity of XAD-7HP resin to PSKB protein hydrolysate was gradually enhanced with the extension of adsorption time (P < 0.05).…”

Section: Study On Static Adsorption Of Protease Hydrolysate From Pskb...mentioning

confidence: 96%

Separation and purification of antioxidant peptides from purple speckled kidney bean by macroporous adsorption resin and analysis of amino acid composition

Yang

et al. 2022

Front. Nutr.

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The protein hydrolysate of purple speckled kidney bean (PSKB) was used as the raw material in this study, and the antioxidant peptide of the PSKB protein hydrolysate was purified using macroporous resin. The XAD-7HP macroporous resin was selected as the best purification material, and the static adsorption-desorption of the purified PSKB antioxidant peptide was optimized. The optimum static adsorption and desorption conditions were as follows: the adsorption capacity reached 11.93 ± 0.11 mg/ml at pH 7 for 24 h, and the desorption capacity was 5.24 ± 0.04 mg/ml with 60% ethanol for 30 min. Under this condition, the amount of antioxidant peptide obtained by adsorption-desorption was the highest. The optimum process conditions were as follows: the appropriate flow rate was 1 ml/min, and the optimal injection volume was 40 ml. The adsorption amount at this time can reach 12.19 ± 0.15 mg/ml. The components with an elution time of 10–30 min were separated using the reversed-phase high-performance liquid chromatography (RP-HPLC) technique to obtain three main components, namely, RP1, RP2, and RP3. The DPPH free radical scavenging ability reached 56.26 ± 0.56, 66.42 ± 0.56, and 78.57 ± 0.56%, respectively, which were 36.65, 46.34 ± 0.56, and 54.39 ± 0.56% higher than those before purification. The amino acid sequences of the three components were identified as Phe-Leu-Val-Asp-Arg-Ile, Phe-Leu-Val-Ala-Pro-Asp-Asp, and Lys-Asp-Arg-Val-Ile-Ser-Glu-Leu.

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Section: Study On Static Adsorption Of Protease Hydrolysate From Pskb...mentioning

confidence: 96%

Separation and purification of antioxidant peptides from purple speckled kidney bean by macroporous adsorption resin and analysis of amino acid composition

Yang

et al. 2022

Front. Nutr.

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Electro-separation of chitooligosaccharides with specific degrees of polymerization using electrodialysis ultrafiltration

Ahmad,

Wang,

Mohsin

et al. 2023

Carbohydrate Polymer Technologies and Applications

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Separation, purification and structural characterization of marine oligosaccharides: A comprehensive and systematic review of chromatographic methods

Ning,

Zhu,

Yao

2024

Journal of Chromatography A

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Adsorption differences and mechanism of chitooligosaccharides with specific degree of polymerization on macroporous resins with different functional groups

Cited by 6 publications

References 50 publications

Separation and purification of antioxidant peptides from purple speckled kidney bean by macroporous adsorption resin and analysis of amino acid composition

Separation and purification of antioxidant peptides from purple speckled kidney bean by macroporous adsorption resin and analysis of amino acid composition

Electro-separation of chitooligosaccharides with specific degrees of polymerization using electrodialysis ultrafiltration

Separation, purification and structural characterization of marine oligosaccharides: A comprehensive and systematic review of chromatographic methods

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