Adsorption Kinetics of an Engineered Gold Binding Peptide by Surface Plasmon Resonance Spectroscopy and a Quartz Crystal Microbalance

Tamerler, Candan; Ören, Ersin Emre; Duman, Memed; Venkatasubramanian, Eswaranand; Sarıkaya, Mehmet

doi:10.1021/la0606897

Cited by 175 publications

(280 citation statements)

References 51 publications

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“…One polypeptide with three repeats of a gold-binding peptide had a K D value of 89 nM (40), and clustering of 24 titania-binding peptides on a ferritin protein increased the binding affinity, giving a K D value of 11 nM (41). Our high affinity antibody fragments showed strong binding with only two loop structures (CDRs 1 and 3).…”

Section: Single Domain Camel Vhh Fragment As a Framework Formentioning

confidence: 99%

High Affinity Anti-inorganic Material Antibody Generation by Integrating Graft and Evolution Technologies

Hattori

Umetsu

Nakanishi

et al. 2010

Journal of Biological Chemistry

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Recent advances in molecular evolution technology enabled us to identify peptides and antibodies with affinity for inorganic materials. In the field of nanotechnology, the use of the functional peptides and antibodies should aid the construction of interface molecules designed to spontaneously link different nanomaterials; however, few material-binding antibodies, which have much higher affinity than short peptides, have been identified. Here, we generated high affinity antibodies from material-binding peptides by integrating peptide-grafting and phage-display techniques. A material-binding peptide sequence was first grafted into an appropriate loop of the complementarity determining region (CDR) of a camel-type single variable antibody fragment to create a low affinity material-binding antibody. Application of a combinatorial library approach to another CDR loop in the low affinity antibody then clearly and steadily promoted affinity for a specific material surface. Thermodynamic analysis demonstrated that the enthalpy synergistic effect from grafted and selected CDR loops drastically increased the affinity for material surface, indicating the potential of antibody scaffold for creating high affinity small interface units. We show the availability of the construction of antibodies by integrating graft and evolution technology for various inorganic materials and the potential of high affinity material-binding antibodies in biointerface applications.Peptides and proteins recognize the interfacial surfaces of their corresponding molecules with high affinity and selectivity because of the multiple-point interactions of hydrogen bonds and salt bridges and the surficial complementarities at the interfaces. Surface recognition by proteins has also been observed in biopolymers in biological systems (1, 2). Furthermore, the use of recent combinatorial library approaches has enabled the identification of short peptides with affinity for nonbiological inorganic materials (3-5). Peptides that bind materials such as metals, metal oxides, and semiconductors have been identified, and they are expected to be useful in bottom-up fabrication procedures in the field of bio-nanotechnology, such as patterning and assembly of proteins and nanomaterials (6 -8), biofunctionalization of nanoparticles (9, 10), and synthesis of crystalline nanometer-sized metal particles (11,12).Besides short peptides, antibodies are becoming attractive as novel material-binding molecules because they have higher affinities and specificities than peptides. Antibodies are recognition molecules with high binding affinity and specificity in the immune system, and they have been used widely in the fields of medical and analytical chemistry (13). By the use of general methodologies with in vivo immune system and in vitro combinatorial selection technologies, antibodies to the surfaces of organic crystals of 1,4-dinitrobenzene (14) and tripeptide (15), magnetite (16), gallium arsenide (17), gold (18), and polyhydroxybutyrate (19) have been identified in immuni...

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Section: Single Domain Camel Vhh Fragment As a Framework Formentioning

confidence: 99%

High Affinity Anti-inorganic Material Antibody Generation by Integrating Graft and Evolution Technologies

Hattori

Umetsu

Nakanishi

et al. 2010

Journal of Biological Chemistry

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“…55,68 Also, it is essential to evaluate quantitatively the degree of peptide specificity for a given solid as compared to nonspecific interactions with other solids of similar chemical compositions or surface structures (e.g., binding to aragonitic versus calcitic CaCO 3 , to gold versus platinum or silver, or to silica versus alumina or titania). 68,69 Ideally, the knowledge of the molecular structure and amino-acid sequence of a GEPI and the basis of the peptide's recognition of the solid will allow for further tailoring and manipulation of its functional properties. 70,71 Acquiring this knowledge, therefore, is an essential step in the design and assembly of functional inorganic structures.…”

Section: Solid Binding and Recognition By Peptides And Assemblymentioning

confidence: 99%

“…84 It is often coupled with quartz-crystal microbalance (QCM) 85 ( Figure 3a). 55,68,83 Significant progress has been made using both SPR 55,68 and QCM. 55,83 However, both of these techniques-especially the latter-have limitations for the study of very small molecules.…”

Section: Solid Binding and Recognition By Peptides And Assemblymentioning

confidence: 99%

“…These include post-selection engineering in which multiple repeats of linear or cyclic architectures for the select sequences are designed and synthesized. 55,68,83 The next step represents a "molecular tool box," which is an inorganic-binding peptide (a) The kinetics of solid binding and specificity of inorganic-binding peptides for a given solid could be determined by surface-plasmon-resonance (SPR) spectroscopy. 83 At the same time, whether a GEPI is specific for a solid surface compared to another solid is determined by quartz-crystal microbalance as well as SPR.…”

Section: Gepi As Molecular Synthesizers Erectors and Assemblers In mentioning

confidence: 99%

“…83 At the same time, whether a GEPI is specific for a solid surface compared to another solid is determined by quartz-crystal microbalance as well as SPR. 68 (b) Spectroscopy, such as circular dichroism 70 and nuclear magnetic resonance, 70 could provide molecular architecture. HABP1 and HABP2 are two hydroxyapatite binding peptides.…”

Section: Gepi As Molecular Synthesizers Erectors and Assemblers In mentioning

confidence: 99%

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Molecular Biomimetics: Genetic Synthesis, Assembly, and Formation of Materials Using Peptides

Tamerler¹,

Sarıkaya²

2008

MRS Bull.

Self Cite

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In nature, the molecular-recognition ability of peptides and, consequently, their functions are evolved through successive cycles of mutation and selection. Using biology as a guide, it is now possible to select, tailor, and control peptide-solid interactions and exploit them in novel ways. Combinatorial mutagenesis provides a protocol to genetically select short peptides with specific affinity to the surfaces of a variety of materials including metals, ceramics, and semiconductors. In the articles of this issue, we describe molecular characterization of inorganic-binding peptides; explain their further tailoring using post-selection genetic engineering and bioinformatics; and finally demonstrate their utility as molecular synthesizers, erectors, and assemblers. The peptides become fundamental building blocks of functional materials, each uniquely designed for an application in areas ranging from practical engineering to medicine.

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Identification and Application of Polymer‐Binding Peptides

Sawada

Serizawa

2013

Peptide Materials

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Adsorption Kinetics of an Engineered Gold Binding Peptide by Surface Plasmon Resonance Spectroscopy and a Quartz Crystal Microbalance

Cited by 175 publications

References 51 publications

High Affinity Anti-inorganic Material Antibody Generation by Integrating Graft and Evolution Technologies

High Affinity Anti-inorganic Material Antibody Generation by Integrating Graft and Evolution Technologies

Molecular Biomimetics: Genetic Synthesis, Assembly, and Formation of Materials Using Peptides

Identification and Application of Polymer‐Binding Peptides

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