Adsorption of bovine serum albumin onto activated carbon prepared from Elaeagnus  stone

Geçgel, Ünal; Üner, Osman

doi:10.4314/bcse.v32i1.5

Cited by 10 publications

(2 citation statements)

References 37 publications

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“…0, No. 0 11 also investigated [121]. Pereira et al [122] prepared activated carbons from cocoa shells and siriguela seeds, and evaluated the whey protein adsorption on produced activated carbon.…”

Section: Activated Carbonmentioning

confidence: 99%

Application of Ion Exchange and Adsorption Techniques for Separation of Whey Proteins from Bovine Milk

Radosavljević

Stanić-Vučinić

Stojadinovic

et al. 2021

CAC

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Background: The world production of whey was estimated to be more than 200 million tons per year. Although whey is an important source of proteins with high nutritional value and biotechnological importance, it is still considered as a by-product of the dairy industry with low economic value due to low industrial exploitation. There are several challenges in the separation of whey proteins: low concentration, the complexity of the material and similar properties (pI, molecular mass) of some proteins. Methods: A narrative review of all the relevant papers on the present methodologies based on ion-exchange and adsorption principles for isolation of whey proteins, known to the authors, was conducted. Results: Traditional ion-exchange techniques are widely used for the separation and purification of the bovine whey proteins. These methodologies, based on the anion or cation chromatographic procedures, as well as combination of aforementioned techniques are still preferential methods for the isolation of the whey proteins on the laboratory scale. However, more recent research on ion exchange membranes for this purpose has been introduced, with promising potential to be applied on the pilot industrial scale. Newly developed methodologies based either on the ion-exchange separation (for example: simulated moving bed chromatography, expanded bed adsorption, magnetic ion exchangers, etc.) or adsorption (for example: adsorption on hydroxyapatite or activated carbon, or molecular imprinting) are promising approaches for scaling up of the whey proteins’ purification processes. Conclusion: Many procedures based on ion exchange are successfully implemented for separation and purification of whey proteins, providing protein preparations of moderate-to-high yield and satisfactory purity. However, the authors anticipate further development of adsorption-based methodologies for separation of whey proteins by targeting the differences in proteins’ structures rather than targeting the differences in molecular masses and pI. The complex composite multilayered matrices, including also inorganic components, are promising materials for simultaneous exploiting of the differences in the masses, pI and structures of whey proteins for the separation.

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Section: Activated Carbonmentioning

confidence: 99%

Application of Ion Exchange and Adsorption Techniques for Separation of Whey Proteins from Bovine Milk

Radosavljević

Stanić-Vučinić

Stojadinovic

et al. 2021

CAC

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“…The phenomenon of protein adsorption is an important process in the development of drug delivery systems, medical implants, and other biomedical applications. Bovine serum albumin (BSA) is commonly used as a model protein for studying the adsorption characteristics of various adsorbents, such as natural clays [1][2][3][4][5][6][7], zeolite [8,9], carbon [10][11][12][13], alginate [14,15], and chitosan [16]. Among these, natural clays and clay minerals have unique properties that make them particularly effective for protein adsorption due to their twodimensional layered structure, swelling, and ion exchange properties [17].…”

Section: Introductionmentioning

confidence: 99%

Modelling and optimization of bovine serum albumin adsorption on calcium bentonite using box-behnken experimental design method

Duranoğlu,

Kumcu Yün

2023

Phys. Scr.

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The purpose of this study is to investigate and optimize the process parameters for bovine serum albumin (BSA) adsorption onto calcium bentonite (CaB) using the Box-Behnken experimental design method. Calcium bentonite was characterized using FT-IR, SEM, XRD, zeta potential measurements, and Boehm titration methods. The BSA adsorption capacity of calcium bentonite was modelled with respect to pH (2.8, 4.8, and 6.8), temperature (25 °C, 32.5 °C, and 40 °C), and initial protein concentration (0.1–1.2 mg ml−1) using the Box-Behnken experimental design method. The Design Expert 13.0 software was used to optimize the process conditions and obtain three-dimensional response surface graphs. A regression model, which gives the relationship between the process parameters and BSA adsorption capacity, was obtained using Design Expert software. The developed model showed that the most effective parameter on adsorption was the initial protein concentration followed by pH and temperature. The maximum adsorption capacity was obtained about 171 mg/g under optimal conditions (pH 4.8, 40 °C, and 1 mg ml−1 of initial BSA concentration). BSA adsorption onto CaB fit the pseudo-second order kinetic model. This study showed that process parameters for BSA adsorption can be effectively investigated and optimized using the Box-Behnken experimental design method with a fewer number of experiments.

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Prepared activated carbon from hazelnut shell where coated nanocomposite with Ag+ used for antibacterial and adsorption properties

Altıntığ

Sarıcı

Karataş

2022

Environ Sci Pollut Res

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In this research prepared activated carbon by H 3 PO 4 from hazelnut shells were coated with silver ions for the preparation of nanoparticles which were mixed in two ratios (1:0.5 and 1:1) by using of chemical reduction method. The adsorption capacity of activated carbons has been proven by BET and iodine number. Then the antimicrobial effect of nanoparticles on the Staphylococcus aureus and Escherichia coli were investigated in addition to that the characterization of hazelnut shell and silver-coated activated carbons were determined by Brunauer-Emmet-Teller (BET), Scanning Electron Microscope (SEM), Fourier Transform Infrared Spectroscopy (FT-IR), and X-ray Diffraction (XRD) methods. The Optimum condition of activated carbon from hazelnut shells indicated that 66.01% carbon content within 36.22% e ciency, while BET surface area achieved as 1208 m 2 / g and its contained 0.6104 cm 3 g -1 total pore volume. The microbial effect indicated that 10 5 CFU/mL of E. coli was completely inhibited in 30 min. Silver-coated activated carbon showed excellent bacteriostatic activity against E. coli and S. aureus. The results show that the composite has good prospects for applications in drinking water. E. coli of 10 4 CFU/mL in drinking water were destroyed within 25 min of contact with the lter made with AgAC. Novelty StatementActivated carbons are used effectively in many environmental applications and rst aimed to obtain activated carbon and then investigate the effectiveness of MB removal and the second stage, we applied antibacterial tests with silver-added activated carbons. We believe that this study will add innovation to the literature with its ease of use in water puri cation by examining the effect of antibacterial tests against time, which differs from other studies.

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Adsorption of bovine serum albumin onto activated carbon prepared from Elaeagnus stone

Cited by 10 publications

References 37 publications

Application of Ion Exchange and Adsorption Techniques for Separation of Whey Proteins from Bovine Milk

Application of Ion Exchange and Adsorption Techniques for Separation of Whey Proteins from Bovine Milk

Modelling and optimization of bovine serum albumin adsorption on calcium bentonite using box-behnken experimental design method

Prepared activated carbon from hazelnut shell where coated nanocomposite with Ag+ used for antibacterial and adsorption properties

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