This paper describes a green one-pot synthesis of L-Serine (L-Ser) capped magnetite nanoparticles (Fe 3 O 4 NPs) and its potential application for adsorption of RhB dye from aqueous solution. The surface property, structure, morphology and magnetic properties of as prepared L-Ser capped Fe 3 O 4 NPs were characterised through UV-Visible spectroscopy, Fourier transform-infrared spectroscopy, X-Ray Diffraction (XRD), scanning electron microscope, transmission electron microscope (TEM) and vibrating sample magnetometer (VSM). The XRD results were indicated the formation of high crystalline spinel type Fe 3 O 4 NPs. TEM images were shown the spherical shape of L-Ser capped Fe 3 O 4 NPs with particle size of 5.9 nm. The VSM curve showed the superparamagnetic behaviour of L-Ser capped Fe 3 O 4 . A plausible interaction mechanism of L-Ser and Fe 3 O 4 NPs was also investigated. L-Ser capped Fe 3 O 4 NPs due to its large surface area and a strong magnetism was shown potential adsorption efficiency towards RhB dye from aqueous solution. The adsorption isotherm data fitted well with Langmuir isotherm model and the monolayer adsorption capacity (q e,exp ) was found to be 6.82 mg/g at pH 7.4 and 300 K. The experimental kinetic data fitted very well with the pseudo-second-order model. The thermodynamic studies reveal that adsorption efficiency is critically dependent on temperature.