Adsorption of Glucose Oxidase to 3‐D Scaffolds of Carbon Nanotubes: Analytical Applications

Nejadnik, M. Reza; Deepak, Francis Leonard; Garcı́a, Carlos D.

doi:10.1002/elan.201000758

Cited by 43 publications

(53 citation statements)

References 75 publications

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“…In agreement with previous reports, MSE < 15 were considered acceptable. 45, 47 The ellipsometric measurements were interpreted using an optical model that considered the dielectric properties of Si (bulk, d = 1 mm) and SiO 2 (d = 2.1 ± 0.5 nm). A standard Cauchy function was added to describe the optical properties of the APTES layer (d = 0.89 ± 0.06 nm).…”

Section: Methodsmentioning

confidence: 99%

Modification of microfluidic paper-based devices with silica nanoparticles

Evans

Gabriel

Benavidez

et al. 2014

Analyst

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147

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This paper describes a silica nanoparticle-modified microfluidic paper-based analytical device (μPAD) with improved color intensity and uniformity for three different enzymatic reactions with clinical relevance (lactate, glucose, and glutamate). The μPADs were produced on Whatman grade 1 filter paper and using a CO2 laser engraver. Silica nanoparticles modified with 3-aminopropyltriethoxysilane (APTES) were then added to the paper devices to facilitate the adsorption of selected enzymes and prevent the washing away effect that creates color gradients in the colorimetric measurements. Here we show three different enzymatic assays for compounds. According to the results herein described, the addition of silica nanoparticles yielded to significant improvements in color intensity and uniformity. The resulting μPADs allowed for the detection of the three analytes in clinically-relevant concentration ranges with limits of detection (LOD) of 0.63 mM, 0.50 mM, and 0.25 mM for lactate, glucose, and glutamate, respectively. An example of an analytical application has been demonstrated for the semi-quantitative detection of all three analytes in artificial urine. The results demonstrate the potential of silica nanoparticles to avoid the washing away effect and improve the color uniformity and intensity in colorimetric bioassays performed on μPADs.

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Section: Methodsmentioning

confidence: 99%

Modification of microfluidic paper-based devices with silica nanoparticles

Evans

Gabriel

Benavidez

et al. 2014

Analyst

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147

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“…Using a similar experimental set-up Nejadnik et al showed that in some cases, enzymes ( e.g. glucose oxidase) can be adsorbed to the interior of the CNT film, obtaining nanocomposites with higher catalytic activities [176] (see. These results support the hypothesis that adsorption can be effectively used to immobilize enzymes to the surface of CNT.…”

Section: Recent Developmentsmentioning

confidence: 99%

Recent applications of carbon-based nanomaterials in analytical chemistry: Critical review

Scida

Stege

Haby

et al. 2011

Analytica Chimica Acta

398

168

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The objective of this review is to provide a broad overview of the advantages and limitations of carbon-based nanomaterials with respect to analytical chemistry. Aiming to illustrate the impact of nanomaterials on the development of novel analytical applications, developments reported in the 2005–2010 period have been included and divided into sample preparation, separation, and detection. Within each section, fullerenes, carbon nanotubes, graphene, and composite materials will be addressed specifically. Although only briefly discussed, included is a section highlighting nanomaterials with interesting catalytic properties that can be used in the design of future devices for analytical chemistry.

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“…This protein was selected as a convenient probe for the adsorption experiments because it allows an easy route to evaluate potential structural modifications affecting its catalytic activity. [16] Additionally, GOx is commercially available and has a well-characterized structure and activity. Therefore, the experiments herein presented describe the adsorption of GOx as a function of applied potential, solution pH, ionic strength, and enzyme concentration, as followed in real time by spectroscopic ellipsometry (SE).…”

Section: Introductionmentioning

confidence: 99%

Adsorption and catalytic activity of glucose oxidase accumulated on OTCE upon the application of external potential

Benavidez¹,

Torrente²,

Marucho³

et al. 2014

Journal of Colloid and Interface Science

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This article describes the adsorption of glucose oxidase (GOx) onto optically transparent carbon electrodes (OTCE) under the effect of applied potential and the analysis of the enzymatic activity of the resulting GOx/OTCE substrates. In order to avoid electrochemical interferences with the enzyme redox center, control electrochemical experiments were performed using flavin adenine dinucleotide (FAD) and GOx/OTCE substrates. Then, the enzyme adsorption experiments were carried out as a function of the potential applied (ranged from the open circuit potential to +950 mV), the pH solution, the concentration of enzyme, and the ionic strength on the environment. The experimental results demonstrated that an increase in the adsorbed amount of GOx on the OTCE can be achieved when the potential was applied. Although the increase in the adsorbed amount was examined as a function of the potential, a maximum enzymatic activity was observed in the GOx/OTCE substrate achieved at +800 mV. These experiments suggest that although an increase in the amount of enzyme adsorbed can be obtained by the application of an external potential to the electrode, the magnitude of such potential can produce detrimental effects in the conformation of the adsorbed protein and should be carefully considered. As such, the article describes a simple and rational approach to increase the amount of enzyme adsorbed on a surface and can be applied to improve the sensitivity of a variety of biosensors.

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Adsorption of Glucose Oxidase to 3‐D Scaffolds of Carbon Nanotubes: Analytical Applications

Cited by 43 publications

References 75 publications

Modification of microfluidic paper-based devices with silica nanoparticles

Modification of microfluidic paper-based devices with silica nanoparticles

Recent applications of carbon-based nanomaterials in analytical chemistry: Critical review

Adsorption and catalytic activity of glucose oxidase accumulated on OTCE upon the application of external potential

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