Adsorption of human albumin and fibrinogen onto heparin-like materials I. Adsorption isotherms

Baillou, N. De; Voegel, Jean‐Claude; Schmitt, A.

doi:10.1016/0166-6622(85)80258-4

Cited by 32 publications

(10 citation statements)

References 32 publications

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“…On positively charged nanogels of pure chitosan, the interface consists of fully hydrated loose polysaccharide chains25 whereas, for the negative ones, the interface bears hydrophobic patches arising from the charge neutralization between chitosan and chondroitin sulfate. The data from the isotherms were fitted with Freundlich's model44 and our results are in favor of a diffusion of the proteins within the bulk of the colloid as no protein/protein interactions could be evidenced. Since these data were obtained in the presence of electrolyte (150 × 10 −3 M ), the counter‐ions release effect was ruled out; hence hydrogen bonds and hydrophobic interactions played a significant role in the binding mechanism.…”

Section: Resultsmentioning

confidence: 88%

“…No saturation of the colloid was observed, in the concentration range investigated. Fitting our data with the Freundlich model:44 (where Γ is the sorption capacity, c the concentration of free protein at equilibrium, K the constant equilibrium and n is the Freundlich exponent), it comes that the Freundlich exponent is close to 1, throughout the experimental concentration range, meaning that there is only one sorption mode of the antibody, depending on the protein/colloid interactions. Therefore, this high sorption capacity is neither due to potentially aggregated proteins at high IgG concentration, nor to a multi layer sorption, which would have required the existence of various protein–protein interactions.…”

Section: Resultsmentioning

confidence: 99%

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Self‐Assemblies on Chitosan Nanohydrogels

Brunel

Véron²,

David

et al. 2010

Macromolecular Bioscience

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Nanohydrogels of pure chitosan, containing neither potentially toxic solvent nor chemical cross-linker, were obtained by an ammonia-induced physical gelation of a reverse emulsion of a chitosan solution in a triglyceride mixture as an organic phase. The resulting colloids were obtained with a controlled size distribution and displayed a positive surface charge. Assemblies with various macromolecules were investigated as a first step toward new nano-carriers for bioactive molecules. Chondroitin sulfate formed polyelectrolyte complexes with the positively charged surface of the nanogels, leading to negative chitosan-based colloidal hydrogels with preservation of the original average size of the dispersion. The mode of assembly of HIV-1 p24 protein with these colloids relied on multiple interactions between the protein and the hydrogels, irrespective of their surface charges. Anyhow, the amounts of loaded protein remained limited, suggesting a surface association. The assembly of an immunoglobulin (IgG) was markedly different from p24. No association was detected with the positive colloidal hydrogels whereas a very high loading capacity could be obtained with the negative ones. So, this work reports that fully biodegradable submicrometric physical hydrogels could be obtained from naturally occurring polymers. These gels could cargo a variety of biomolecules making them versatile carriers with many potential applications in Life Sciences.

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Section: Resultsmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Self‐Assemblies on Chitosan Nanohydrogels

Brunel

Véron²,

David

et al. 2010

Macromolecular Bioscience

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“…The adsorption of chitosan from a sodium acetate buffer was tentatively fitted with the Langmuir model [18] or the Freundlich one [19] but none of them applied, suggesting that many different kinds of interactions were involved. Interestingly, the isotherms obtained in ammonium acetate could be fitted by the Freundlich model which writes:…”

Section: Effect Of External Parameters On the Adsorption Of Chitosanmentioning

confidence: 99%

“…The plots of log G versus log C for each isotherm obtained in an ammonium acetate buffer reported in Figure 4 are characterized by a break in the initial slope corresponding to two different adsorption regimes. [19] The first one, at low chitosan concentration, ended at a critical value, C c , for which the second regime took place. These two regimes suggest a difference in conformation of the adsorbed polymer at the surface of the particles.…”

Section: Effect Of External Parameters On the Adsorption Of Chitosanmentioning

confidence: 99%

“…[19] Other parameters like interactions between polymer molecules adsorbed at the interface and/or the solution conformation of the polymer chain have to be taken into consideration. The amount of adsorbed chitosan being 0.13 mg Á m À2 (PLA-P10, Table 1), the area occupied by a molecule is 0.25 Â 10 À17 m 2 .…”

Section: Effect Of External Parameters On the Adsorption Of Chitosanmentioning

confidence: 99%

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Adsorption of Chitosan onto Poly(D,L‐lactic acid) Particles: A Physico‐Chemical Investigation

Messai¹,

Delair²

2005

Macro Chemistry & Physics

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Summary: Particles of poly(D,L‐lactic acid) (PLA) obtained by emulsification‐diffusion were surface‐modified by adsorption of chitosan. Thus, positively charged biodegradable carriers were obtained as potential carriers for DNA. Various physico‐chemical parameters, susceptible to impact the elaboration of such colloids, were investigated. External parameters were the ionic strength and the nature of the buffer; pH was fixed at 4.5 in order to ensure the ionisation of both counterparts. Internal parameters were the molar mass of chitosan and its degree of acetylation (DA). Kinetically, the adsorption of chitosan was spontaneous. Adsorption isotherms of chitosan were established using two different assays for chitosan, one based on the formation of a covalent bond, and the other on the formation of an ion pair. Two adsorption regimes were evidenced in ammonium acetate buffer. The amount of adsorbed chitosan increased with its molar mass up to a limit due to steric hindrance and with the DA. The latter effect could be explained by a reduction of the molecular dimensions of chitosan with DA. On storing, desorption of chitosan was dependent on the molar mass of the polysaccharide and, to a lesser extent, on the DA.Complexation isotherm of chitosan onto PLA particles.imageComplexation isotherm of chitosan onto PLA particles.

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Silicone Oil in Biopharmaceutical Containers: Applications and Recent Concerns

Dixit

Kalonia

2014

Concise Encyclopedia of High Performance Silicones

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Adsorption of human albumin and fibrinogen onto heparin-like materials I. Adsorption isotherms

Cited by 32 publications

References 32 publications

Self‐Assemblies on Chitosan Nanohydrogels

Self‐Assemblies on Chitosan Nanohydrogels

Adsorption of Chitosan onto Poly(D,L‐lactic acid) Particles: A Physico‐Chemical Investigation

Silicone Oil in Biopharmaceutical Containers: Applications and Recent Concerns

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