Adsorption of human serum albumin in porous silicon gradients

Karlsson, Linda; Tengvall, Pentti; Lundström, Ingemar; Arwin, Hans

doi:10.1002/pssa.200306518

Cited by 16 publications

(15 citation statements)

References 7 publications

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“…It was found that PS may be tailored to be bioactive and biocompatible material, and thus it is suitable for implantation into a living organism. Some researchers have explored the PS as biodegradable material for slow release of trace elements or drugs and for studying of surface-protein interactions [31,49]. PS has also received considerable interest for applications in medicine due to its solubility in physiologic fluids [24].…”

Section: Ha Formation Through Llsi Processmentioning

confidence: 99%

Stimulated in vitro bone-like apatite formation by a novel laser processing technique

Pecheva

Petrov

Lungu

et al. 2008

Chemical Engineering Journal

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Section: Ha Formation Through Llsi Processmentioning

confidence: 99%

Stimulated in vitro bone-like apatite formation by a novel laser processing technique

Pecheva

Petrov

Lungu

et al. 2008

Chemical Engineering Journal

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“…By careful fine-tuning the porous silicon carrier properties it is possible 3 to attain zero order drug release kinetics and develop novel pharmaceutical devices such as insulin pumps [14,15] and gastrointestinal patches for oral insulin delivery [16,17]. Some recent studies have studied protein loading into porous silicon layers [18][19][20][21], and there is a general consensus that this is a complex process that can be influenced by the morphology of the silicon device, its nanostructure, and the device surface chemistry [22]. However a systematic approach would improve our understanding of the physicochemical processes involved in protein-porous silicon interaction, and ultimately, it would lead to optimized formulations for protein delivery.…”

Section: Introductionmentioning

confidence: 99%

Protein delivery based on uncoated and chitosan-coated mesoporous silicon microparticles

Pastor

Matveeva

Valle-Gallego

et al. 2011

Colloids and Surfaces B: Biointerfaces

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Mesoporous silicon is a biocompatible, biodegradable material that is receiving increased attention for pharmaceutical applications due to its extensive specific surface. This feature enables to load a variety of drugs in mesoporous silicon devices by simple adsorption-based procedures. In this work, we have addressed the fabrication and characterization of two new mesoporous silicon devices prepared by electrochemistry and intended for protein delivery, namely: (i) mesoporous silicon microparticles and (ii) chitosan-coated mesoporous silicon microparticles. Both carriers were investigated for their capacity to load a therapeutic protein (insulin) and a model antigen (bovine serum albumin) by adsorption. Our results show that mesoporous silicon microparticles prepared by electrochemical methods present moderate affinity for insulin and high affinity for albumin. However, mesoporous silicon presents an extensive capacity to load both proteins, leading to systems were protein could represent the major mass fraction of the formulation. The possibility to form a chitosan coating on the microparticles surface was confirmed both qualitatively by atomic force microscopy and quantitatively by a colorimetric method.Mesoporous silicon microparticles with mean pore size of 35 nm released the loaded insulin quickly, but not instantaneously. This profile could be slowed to a certain extent by the chitosan coating modification. With their high protein loading, their capacity to provide a controlled release of insulin over a period of 60-90 min, and the potential mucoadhesive effect of the chitosan coating, these composite devices comprise several features that render them interesting candidates as transmucosal protein delivery systems.

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“…The enhancement of biochemical signatures in the infrared has been demonstrated by surface area enhancement in porous silicon. [5][6][7][8][9][10][11] Ex situ optical characterization of porous alumina has been performed in the visible range using ellipsometry, 12,13 and oligonucleotide adsorption to functionalized porous alumina has been monitored in real time with optical reflectivity.…”

Section: Introductionmentioning

confidence: 99%

Optical characterization of porous alumina from vacuum ultraviolet to midinfrared

Thompson

Snyder

Castro

et al. 2005

Journal of Applied Physics

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Porous alumina was fabricated and optically characterized over a wide spectral range. Layers were formed electrochemically in oxalic acid solution from 10-m-thick aluminum films evaporated onto silicon wafers. The layer formation was monitored with in situ spectroscopic ellipsometry in the visible and near-infrared wavelength range to accurately determine the thickness and dielectric functions. Anisotropy due to the columnar nature of the porous structure was determined using optical modeling. The porous alumina layer was found to have a small but significant absorption tail throughout the visible region. Atomic force microscopy and scanning electron microscopy were used throughout the process to assess the quality of pore formation. The mean pore center-to-center spacing was approximately 100 nm with thicknesses up to 5 m. The infrared spectra revealed absorption peaks previously seen in ceramic alumina and peaks not associated with bulk alumina.

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Adsorption of human serum albumin in porous silicon gradients

Cited by 16 publications

References 7 publications

Stimulated in vitro bone-like apatite formation by a novel laser processing technique

Stimulated in vitro bone-like apatite formation by a novel laser processing technique

Protein delivery based on uncoated and chitosan-coated mesoporous silicon microparticles

Optical characterization of porous alumina from vacuum ultraviolet to midinfrared

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