2018
DOI: 10.1021/acs.jced.8b00535
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Adsorption of IgG and BSA on Two Chromatographic Resins—Poly(ethylenimine)-4FF Resin and Tetrapeptide-poly(ethylenimine)-4FF Resin

Abstract: Poly­(ethylenimine) (PEI) as a long flexible chain has been well used in protein chromatography with the advantages of high spatial utilization and “chain delivery” effect. In this work, two resins, i.e., poly­(ethylenimine)-4FF resin (PEI-4FF resin) and Ac-YFRH-PEI-4FF resin by grafting tetrapeptide to PEI-4FF resin, were prepared for the protein adsorption and chromatographic separation. The pH-dependent adsorption properties of PEI-4FF resin and Ac-YFRH-PEI-4FF resin were discovered by getting the data of a… Show more

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Cited by 1 publication
(3 citation statements)
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“…Since the lower K d means the higher binding ability, therefore, all results showed the increase of the pH enhanced the binding ability between hIgG and the resin (Table 1). Figure 2B showed this resin had a stable binding ability to BSA at pH 5.0–8.0 compared to the hIgG that might be caused by the isoelectric points of hIgG (pH 6.5) and BSA (pH 4.8) [16]. Moreover, compared to the non‐grafted Ac‐YFRH‐4FF resin (pH 7.0, Q m = 80 mg hIgG/g resin, K d = 7.00 mg/mL), Ac‐YFRH‐XL resin showed much lower K d (pH 7.0, Q m = 81 mg hIgG/g resin, K d = 0.13 mg/mL), which indicated stronger binding ability of dextran‐grafted resin due to its “chain transfer” power [26].…”
Section: Resultsmentioning
confidence: 99%
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“…Since the lower K d means the higher binding ability, therefore, all results showed the increase of the pH enhanced the binding ability between hIgG and the resin (Table 1). Figure 2B showed this resin had a stable binding ability to BSA at pH 5.0–8.0 compared to the hIgG that might be caused by the isoelectric points of hIgG (pH 6.5) and BSA (pH 4.8) [16]. Moreover, compared to the non‐grafted Ac‐YFRH‐4FF resin (pH 7.0, Q m = 80 mg hIgG/g resin, K d = 7.00 mg/mL), Ac‐YFRH‐XL resin showed much lower K d (pH 7.0, Q m = 81 mg hIgG/g resin, K d = 0.13 mg/mL), which indicated stronger binding ability of dextran‐grafted resin due to its “chain transfer” power [26].…”
Section: Resultsmentioning
confidence: 99%
“…Further, the highest binding capacity could reach to 133 mg/g with low K d (0.03 mg/mL) at pH 8.9, which even showed better binding ability to non‐grafted resin with the highest binding capacity of 100.5 mg/g [12], indicating the potential advantages of grafted resin for antibody purification. Moreover, compared to the Ac‐YFRH‐PEI‐4FF resin [16] that used PEI as the spacer arm, Ac‐YFRH‐XL resin was less affected by the spacer arm when binding the target protein. The branched chain charge of PEI could affect the binding ability between the tetrapeptide ligand and target protein.…”
Section: Resultsmentioning
confidence: 99%
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