Adsorption of immunoglobulin G, protein A, and fibronectin in the submonolayer region evaluated by a combined study of ellipsometry and radiotracer techniques

Jönsson, Ulf; Malmqvist, Magnus; Rönnberg, Inger

doi:10.1016/0021-9797(85)90115-8

Cited by 88 publications

(33 citation statements)

References 28 publications

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“…Other methods such as radiolabeling [203] and scanning force microscopy [204] also exist. Compared to those techniques, a fiber optic sensor has the advantage of miniature size thus allowing the use of a small sample volume.…”

Section: Introductionmentioning

confidence: 99%

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

et al. 2005

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(ABSTRACT)Fiber-optic Fabry-Perot interferometric (FFPI) sensors have been widely used due to their high sensitivity, ease of fabrication, miniature size, and capability for multiplexing. However, direct measurement of self-assembled thin films, receptor immobilization process or biological reaction is limited in the FFPI technique due to the difficulty of forming Fabry-Perot cavities by the thin film itself. Novel methods are needed to provide an accurate and reliable measurement for monitoring the thin-film growth in the nanometer range and under various conditions. In this work, two types of fiber-optic multicavity Fabry-Perot interferometric (MFPI) sensors with built-in temperature compensation were designed and fabricated for thin-film measurement, with applications in chemical and biological sensing. Both the tubing-based MFPI sensor and microgap MFPI sensor provide simple, yet high performance solutions for thin-film sensing. The temperature dependence of the sensing cavity is compensated by extracting the temperature information from a second multiplexed cavity. This provides the opportunity to examine the thin-film characteristics under different environment temperatures.To demonstrate the potential of this structure for practical applications, immunosensors were fabricated and tested using these structures. Self-assembled polyelectrolytes served as a precursor film for immobilization of antibodies to ensure they retain their biological activity. This not only provides a convenient method for protein immobilization but also presents the possibility of increasing the binding capacity

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Section: Introductionmentioning

confidence: 99%

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

et al. 2005

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“…Many kinds of methods are used to study protein adsorption on surfaces, such as ellipsometry [11][12][13][14], variable angle reflectometry [15,16], fluorescence spectroscopy [17], and atomic force microscopy [18]. Among these, ellipsometry is a nondestructive, optical-surface-sensitive method for the investigation of protein adsorption mainly at metal surfaces [12].…”

Section: Introductionmentioning

confidence: 99%

Adsorption of human serum albumin onto gold: a combined electrochemical and ellipsometric study

Ying

Viana

Abrantes

et al. 2004

Journal of Colloid and Interface Science

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Human serum albumin adsorption onto gold surfaces was investigated by electrochemical and ellipsometric methods. Albumin adsorption onto gold was confirmed by the change of the open circuit potential of gold and by the ellipsometric parameter variation during albumin immobilization. In both experiments the parameters reached stable values within 10-15 min. The albumin adsorption layer thickness measured with the ellipsometer was about 1.5 nm. The adsorption of albumin under applied potential was also investigated and it was found that both positive and negative applied potential promote albumin adsorption. Changes in the optical parameters of bare gold and albumin adsorbed onto gold surface under applied potential were investigated with in situ ellipsometry. The similarity and reversibility of the optical changes showed that adsorbed albumin was stable on the gold surface under the applied potential range (−200-600 mV). The cyclic voltammograms of K 3 Fe(CN) 6 on the modified gold surface showed that albumin could partly block the oxidation and reduction reaction.  2004 Elsevier Inc. All rights reserved.

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“…www.intechopen.com Recently, SE has been extensively used for the characterization of organic materials and biomaterials [75,76]. With the development of ellipsometry, it is now possible to obtain monolayer spectroscopy, e.g.…”

Section: Application Of Ellipsometry In Stoichiometry 41 Surface Andmentioning

confidence: 99%