Adsorption of norovirus and ostreid herpesvirus type 1 to polymer membranes for the development of passive samplers

Vincent-Hubert, Françoise; Morga, Benjamín; Renault, Tristan; Guyader, Françoise S. Le

doi:10.1111/jam.13394

Cited by 32 publications

(28 citation statements)

References 42 publications

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“…The method developed here gives integrated sampling over time for the more frequently detected microorganisms, AllBac, NoV, and Vibrio spp., which is a characteristic of passive samplers in general (Taylor et al, 2019). These observations are consistent with our laboratory experiments in which we observed that the concentration of NoV on membranes increased with the duration of exposure (Vincent-Hubert et al, 2017). The integrative nature of microorganism sampling using membranes is also shown for an enveloped virus, OsHV-1, supposedly present at low concentrations in the marine environment.…”

Section: Discussionsupporting

confidence: 91%

“…Our data illustrate the capacity of viruses to attach to polymer surfaces, making it possible to investigate the presence of viruses in the plastisphere. We previously demonstrated that viruses (NoV and OsHV-1) are adsorbed onto polymer surfaces and we depicted their adsorption rate on membranes and their stability, which can explain our current observations (Vincent-Hubert et al, 2017). Attachment of NoV onto membranes is probably facilitated by its interaction with bacteria and particulate matter [review in Amarasiri and Sano (2019)].…”

Section: Discussionsupporting

confidence: 66%

“…Three different membranes, LDPE, Zetapor, and nylon, were used as passive samplers based on data obtained in a previous study (Vincent-Hubert et al, 2017). Zetapor filter (0.45 µm), an electropositive, charge-modified diatomaceous earth/cellulose filter, was purchased from 3M (Cergy-Pontoise, France), LDPE (thickness of 80 µm) from Manutan (France), and nylon nets (thickness of 100 µm) from Mougel (France).…”

Section: Preparation Of Passive Samplersmentioning

confidence: 99%

“…Membranes were rinsed in sterile water and nucleic acids were directly extracted from the membrane, the entire membrane was used for extraction. For the first field study, a NucliSENS extraction kit (bioMérieux, Lyon France) was used for NoV, Vibrio spp., and Bacteroidales markers, and a QiAamp tissue mini kit R (QIAgen, France) was used for OsHV-1 as published previously (Vincent-Hubert et al, 2017). For the second field study, a single extraction procedure was used for all microorganisms using a NucliSENS extraction kit.…”

Section: Nucleic Acid Extractionmentioning

confidence: 99%

“…Concerning microorganisms, very little use has been made of passive sampling; examples include the detection of poliovirus in wastewater or of norovirus in continental waters with gauze as the adsorption membrane (Fattal and Katzenelson, 1976;Tian et al, 2017). We have developed passive samplers constituted of membranes with capacity to adsorb viruses and bacteria (Vincent-Hubert et al, 2017). Following the adsorption phase, the detection of nucleic acids of different microorganisms is performed by PCR.…”

Section: Introductionmentioning

confidence: 99%

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Passive Samplers, a Powerful Tool to Detect Viruses and Bacteria in Marine Coastal Areas

Vincent-Hubert

Wacrenier

Morga³

et al. 2021

Front. Microbiol.

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The detection of viruses and bacteria which can pose a threat either to shellfish health or shellfish consumers remains difficult. The current detection methods rely on point sampling of water, a method that gives a snapshot of the microorganisms present at the time of sampling. In order to obtain better representativeness of the presence of these microorganisms over time, we have developed passive sampling using the adsorption capacities of polymer membranes. Our objectives here were to assess the feasibility of this methodology for field detection. Different types of membrane were deployed in coastal waters over 2 years and the microorganisms tested using qPCR were: human norovirus (NoV) genogroups (G)I and II, sapovirus, Vibrio spp. and the species Vibrio alginolyticus, V. cholerae, V. vulnificus, and V. parahaemolyticus, OsHV-1 virus, and bacterial markers of fecal contamination. NoV GII, Vibrio spp., and the AllBac general Bacteroidales marker were quantified on the three types of membrane. NoV GII and OsHV-1 viruses followed a seasonal distribution. All membranes were favorable for NoV GII detection, while Zetapor was more adapted for OsHV-1 detection. Nylon was more adapted for detection of Vibrio spp. and the AllBac marker. The quantities of NoV GII, AllBac, and Vibrio spp. recovered on membranes increased with the duration of exposure. This first application of passive sampling in seawater is particularly promising in terms of an early warning system for the prevention of contamination in oyster farming areas and to improve our knowledge on the timing and frequency of disease occurence.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 66%

Section: Preparation Of Passive Samplersmentioning

confidence: 99%

Section: Nucleic Acid Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Passive Samplers, a Powerful Tool to Detect Viruses and Bacteria in Marine Coastal Areas

Vincent-Hubert

Wacrenier

Morga³

et al. 2021

Front. Microbiol.

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Viruses and Non‐Woven Polymers: Surface Properties and Future Perspectives in Sampling for Wastewater‐Based Epidemiology

Ahmedullah,

Whiley,

Fallowfield

et al. 2024

Adv Materials Inter

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Environmental disease monitoring initiatives such as wastewater‐based epidemiology can offer a unique perspective on the health status of a population. Such efforts are being increasingly utilized to guide public health initiatives and to aid in controlling the spread of infectious diseases. Key to these approaches is the sampling and identification of viruses, bacteria, and other pathogens. Advanced material technologies can be explored for the development of materials suitable for sampling, leading to the retention and detection of viruses. Here, how the surface interactions between viruses and adsorbent materials can inform the future development of effective, novel materials to aid in sampling viruses for wastewater‐based epidemiology are considered. This review provides a summary of the surface properties of viruses along with their physiochemical interactions with adsorbent materials at the solid‐water interface. Also discussed are the properties of non‐woven polymer membranes, a newer material technology being employed for the retention of viruses, with a focus on virus‐capture applications in aqueous environments.

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Microbial Dynamics and Quality Monitoring in Biopharmaceutical Production

Pashang,

Gilbride,

Wenk

2024

ChemBioEng Reviews

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Prokaryotic cells are pivotal in meeting the global demand for biopharmaceuticals. However, challenges such as the absence of advanced technology for real‐time monitoring, standardized testing methodologies, and quality risk assessment of microbial activity have led to increased production costs, delays, and shortages of biopharmaceutical products. A thorough understanding of how biomolecule production interacts with microbial population structure and function is vital for improving continuous manufacturing and process automation. In this review, we discuss the current microbiological techniques that meet good manufacturing practice requirements in industrial settings, explore the advantages of monitoring and measuring biomass growth efficiency and turnover rates beyond regulatory criteria for product release, and provide a critical assessment of the current state of knowledge on bioassays and engineering tools for biomolecule yield measurement and monitoring. Furthermore, we identify areas for future development, potential applications, and the need for interdisciplinary innovation to drive future research, including advancing bioassays for biopharmaceutical wastewater risk.

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Adsorption of norovirus and ostreid herpesvirus type 1 to polymer membranes for the development of passive samplers

Abstract: Membranes tested will be used as passive samplers to improve the detection of virus in oyster production areas. Also, passive samplers could be a valuable tool for microbiome analysis with new generation sequencing.

Cited by 32 publications

References 42 publications

Passive Samplers, a Powerful Tool to Detect Viruses and Bacteria in Marine Coastal Areas

Passive Samplers, a Powerful Tool to Detect Viruses and Bacteria in Marine Coastal Areas

Viruses and Non‐Woven Polymers: Surface Properties and Future Perspectives in Sampling for Wastewater‐Based Epidemiology

Microbial Dynamics and Quality Monitoring in Biopharmaceutical Production

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